- Biologics Analysis by CESI-MS
- CESI Proteomics
- CESI Metabolomics
- Drugs and their metabolites in biofluids
Advantages of CESI-MS Ultra-low flow separation-ESI
- High-resolution separation without a stationary phase - no small peptides lost in the column flow-through and no large peptides retained
- ESI flow at
- Non-tapered, open-tube capillary, with no dead volumes - maximizing peak efficiency and sensitivity
- No time lost on minimizing void volumes and dealing with clogging
- No sample carry-over
- Capable of multiple injections from < 5 uL sample volumes
- Can be operated as a standalone CE
- Orthogonal and/or superior coverage to other technologies
CESI integrates high efficiency capillary electrophoresis (CE) with electrospray ionization (ESI).This technique allows for ultra-low-flow < 25 nL/min, into the mass spectrometer resulting an an overall increase in ionization efficiency.
Easy-to-use OptiMS cartridge
- Plug-and-spray design
- Interfacing with MS is fast and easy– adapters available for (list models)
- Open-tube capillary design doesn't require lengthy column equilibration
- Decreased injection-to-injection time
CESI mobile design
- Equipped with a height adjustable mobile bench
- Switching between LC-MS and CESI-MS has been made swift and simple
|High performance separation and |
ESI module upstream from MS
Improved ionization efficiencies result in reduced ion suppression and increased overall sensitivity.
Designed for mass spectrometry applications that analyze charged and polar molecules, the CESI 8000 is the next evolution in technology.With CESI-MS the advantages of CE are delivered without dilution or disturbance to the mass spectrometer.Stable ultra-low flow ESI serves to reduce ion suppression and increases sensitivity.
Unleash the power of CE separation for the best in mass spectrometry detection.
The OptiMS cartridge assembly consists of a separation capillary terminating in the porous sprayer, a conductive liquid capillary, circulating liquid cooling, and sprayer housing.Conductive fluid is delivered automatically from a system vial that completes the CE circuit.This capillary is housed in a protective cartridge that allows easy transfer and routine use in a rugged and robust manner.With the CESI 8000 system, the cartridge simply clicks into place creating an easy-to-use plug-and-spray set up for your mass spectrometry.
The CESI 8000 was developed in collaboration with mass spectrometry researchers covering a number of applications.They sought to expand their range of detection and increase sensitivity.In order to achieve this goal, a low flow pre-MS separation technology was integrated with novel ESI coupling technology.
Swift and simple "plug-and-spray" design increases operational efficiency
- Automated sample handling from microvials and nanoVials
- Simple interfacing with automatic triggering of MS data collection
- Easy switching between liquid chromatography (LC) and CESI-MS
The sprayer, located within a protective housing on the CESI 8000 Plus, combines an intrinsically low-flow CE separation with electrospray ionization (ESI) within a single, simple device that has no liquid junction or dead volume.
- Capillary's distal end is porous to allow ion flow
- Electrical contact for the CE is achieved through an ESI needle filled with conductive fluid
- ESI's electrical contact is achieved through the protruding capillary tip
- Low flow at the tip terminus instantly generates a fine spray when ESI voltage is applied
- All electrochemistry associated with electrolysis of water is hereby decoupled from the spray
- Capillary inlet and exit are of the same dimension, reducing the clogging often associated with nanoscale techniques
- ESI is generated at lower voltages, which reduces the risks of oxidation artifacts
1Kelly RT, Page JS, Zhao R, Qian W, Mottaz HM, Tang K, Smith RD, Anal.Chem.2008, 80, 143-149
2Schmidt A, Karas M, Dulks T, J. Am.Soc.Mass Spectrom.2003, 14, 492-500
3Reprinted with permission from "High Capacity Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry:Coupling a Porous Sheathless Interface with Transient-Isotachophoresis" by Jean-Marc Busnel et al. (2010) Analytical Chemistry 82 (22), 9476-9483.