The SCIEX SelexION Device is an innovative achievement in differential ion mobility spectrometry. The SelexION Ion Mobility Spectrometry Tool has the reproducibility, robustness, and ease-of-use to deliver highly-selective, quantitative and qualitative analyses; the SelexION is compatible with UHPLC time scales over multiple MRM transitions simultaneously – one of the world's most sensitive triple quadrupole instruments and QTRAP Platforms.
For scientists who are challenged by assays with isobaric interferences and difficult-to-separate, co-eluting contaminants, this fast, reproducible, and easy-to-use approach will enhance the selectivity of your LC/MS/MS separations.
SelexION Technology takes one of the world's most sensitive triple quadropole instrument and the QTRAP System and adds a new dimension of selectivity to enhance quantitative and qualitative performance.
|Scan Speed||The device can acquire data for a single MRM transition in 25 msec, including the inter-scan pause time of 20 msec. Four-fold faster than cylindrical ion mobility. Amenable to multiple analyte analysis and UHPLC time scales.|
|Tuning Stability||The optimum compensation voltage (COV) does not shift by more than 10% of the DMS peak width (full width at half height, typically 2.5V) at a fixed separation voltage (SV) over 24 hours on an equilibrated system operating under standard DMS resolution gas setting. Suitable for quantitative/regulated lab applications.|
|Integrated Chemical Modifier||Can increases the ion mobility separation power by up to 10-fold.|
|Mounting and Dismounting||User mountable and dismountable without the need for tools or breaking vacuum. Compact design takes less than two minutes to install or remove at room temperature.|
|Scheduled MRM™ Algorithm Support||Compatible with the Scheduled MRM™Algorithm that provides automatic, dynamic adjustment of MRM transitions during acquisition, such that only compounds that elute within a specified time window are monitored, allowing the system to dynamically schedule MRM scans during the experiment.|
|Reproducibility & Robustness||Proven to meet regulated bioanalysis guidelines.|
|A Clear Difference||Injection of clenbuterol at 5 pg/µL in urine. (a) High chemical noise makes detection impossible. (b) Differential ion mobility spectrometry with SelexION™ Technology increases selectivity and eliminates the high background, producing a 10-fold improvement in signal-to-noise.|
|Background-Checked||Stable-isotope-labeled synthetic peptides generated for quantitation of a therapeutic IgG in digested human plasma showed much cleaner MRM signals at the same on-column amount when an ion mobility separation was performed. This yielded a five-fold improvement in the LLOQ that was achieved on-column.|
|Above the Noise||Significantly reduce and eliminate isobaric intereferences and co-eluting contaminants. Grapefruit extract fortified with conazole degradant products at 0.01 mg/kg. Samples were kindly supplied by R.Schoening, Bayer CropScience, Monheim, Germany and J.Jasak, Technical University, Institute of Food Chemistry, Dresden, Germany.|
|Multiplied Selectivity||Improving quantitation by reducing structurally-similar interferences. A 30 µL injection of eicosanoids standard mix containing prostaglandin F2α (PGF2α). (a) Interference with structurally-similar species leads to difficulties in identification and quantitation. (b) SelexION™ Technology eliminates isobaric interferences and chemical background.|