The SCIEX clinical diagnostic portfolio is For In Vitro Diagnostic Use. Rx Only. Product(s) not available in all countries. For information on availability, please contact your local sales representative or refer to https://sciex.com/diagnostics. All other products are For Research Use Only. Not for use in Diagnostic Procedures.
Trademarks and/or registered trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners in the United States and/or certain other countries.
© 2020 DH Tech. Dev. Pte. Ltd.
Amplifex Keto Reagent

Amplifex Keto Reagent

Amplifex Keto Reagent

Amplifex™ Keto Reagent

For research use only. Not for use in diagnostic procedures.

Signal enhancement maximized through intelligent chemistry

Amplifex Keto Reagent is designed specifically to enhance signal strength up to 500-fold (depending on the analyte). 

The Amplifex Keto Reagent is a universal reagent that reacts with a molecule's keto or aldehyde functional group, such as the ketone in ketosteroids (e.g., testosterone). 

With the Amplifex Keto Reagent,  your sample size can be dramatically reduced, while still achieving LLOD and LLOQ levels that were difficult, if not impossible, by mass spectrometry analysis even on the most-sensitive platforms.

  • Intelligent design boosts ionization efficiency and improves fragmentation
  • Substantial reduction in sample volume and analysis time is enabled.
  • Reactions are simple (one-step derivatization) and fast (30-60 minutes).
  • Universal derivatization technique reacts with all molecules containing the keto or aldehyde functional groups

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Overview

Designed specifically to enhance sensitivity of trace analytes and enable workflows that push the limits of sample volume

Amplifex Keto is a customized derivatizing reagent designed to react specifically with a molecule's keto and aldehyde functional groups  to improve ionization efficiency.   (i.e. Amplifex™ Keto Reagent treatment results in a sensitivity enhancement of ~40-fold in testosterone and universally reacts with all ketosteroids across an array of sources (plasma, dried blood spot, dialyzed samples, urine, and oral fluids).

 

Example Explanation
Amplifex Keto Reagent: Signal enhancement for testosterone

Amplifex Keto Reagents

 Amplifex Keto Reagent is designed to boost signal intensity for all ketosteroids. Here is an example of a 40-fold increase in sensitivity for testosterone.
Amplifex Keto Reagent: Signal enhancement for molecules with keto or aldehyde functional groups.

Amplifex Keto Reagents

 Amplifex Keto Reagent is intelligently designed to derivatize any molecule's keto or aldehyde functional groups. See examples of additional compounds.
Amplifex Keto Reagent derivatizes across an array of matrices

Amplifex Keto Reagents

 Amplifex Keto Reagent is not limited to one sample matrix. The reagent will derivatize molecules in dried blood spots, plasma, dialyzed samples, urine, and oral fluids.
Amplifex Keto Reagent derivatizes across an array of matrices

Amplifex Keto Reagents

 Amplifex Keto Reagent is not limited to one sample matrix. The reagent will derivatize molecules with dried blood spots, plasma, dialyzed samples, urine, and oral fluids.
Amplifex Keto Reagent workflow

Amplifex Keto Reagents

 The Amplifex Keto Reagent derivatization and sample preparation workflows are quick (30 minutes), simple (one-step), reproducible, robust, and high-yielding regardless of sample matrix.

RUO-MKT-18-1996-A

Kits
Part NumberKit Name/Description
4465962 Amplifex Keto Reagent Kit
Contains a protocol, 2 tubes of Amplifex Keto reagent, and 2 tubes of diluent. Each tube contains enough reagent to complete 25 reactions
4466055 Amplifex Keto Prep Starter Kit
Contains hardware components required for Amplifex Keto sample prep. Includes 24-pack C-200 cartridges, 24 unit rack, and protocol.
4466054 Amplifex Keto Cartridge Kit
Contains cartridges for Amplifex Keto sample prep. Includes 24-pack C-200 cartridges and sample prep protocol.
4466179 Amplifex Keto Plate Kit
Contains components required for increased sample throughput. Includes one 96-well plate with C-200 media, one collection plate, and protocol.
5028174 Amplifex Prep Replacement Solvent Guide
12-pack of replacement solvent guides.
Resources
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Title Date
Protocol for Amplifex Prep - C200 96 Well kit 11/01/2012
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The SCIEX clinical diagnostic portfolio is For In Vitro Diagnostic Use. Rx Only. Product(s) not available in all countries. For information on availability, please contact your local sales representative or refer to https://sciex.com/diagnostics. All other products are For Research Use Only. Not for use in Diagnostic Procedures.
Trademarks and/or registered trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners in the United States and/or certain other countries.
© 2020 DH Tech. Dev. Pte. Ltd.