LC/MS/MS is ideally suited to identify panels of steroids, vitamin D and its metabolites, peptides or proteins, and other compounds in complex matrices such as blood, urine, saliva and whole cell lysates.
Unlike conventional immunoassay methods, LC/MS/MS enables highly selective and specific analysis of multiple compounds in a single run, leading to more confidence in results and opening up new discoveries.
Examples of research applications includes:
One key advantage of LC/MS/MS analysis is its ability to analyze a wide variety of compounds, even across different compound classes. For example, you can easily combine analysis of testosterone, estrogens, and mono-OH-vitamin D into a single method. You can also add other steroids, such as OH-progesterone, cortisol, and DHEA into an LC/MS/MS method, and quantitate a panel of steroids in a single assay.
Chromatogram of steroid panel analysis