Technical Note: Rapid Metabolite Identification Workflow using MetabolitePilot Software
Hepregen MetID Infographic
Understanding the metabolism of your compound(s) by identifying all metabolites formed in pre-clinical development studies is mandated by all regulatory agencies and is essential for interpreting your pharmacology, pharmacokinetic and toxicology data. In addition, while it is possible to screen and eliminate labile compounds through traditional metabolic stability assays, it is also crucial to identify the metabolic soft-spots on the compound that are responsible for accelerated metabolism. Access to such metabolic soft-spot data is invaluable in accelerating the discovery process as it provides medicinal chemists the information they need to re-engineer more viable compounds.
A Comprehensive, One-stop Solution for Metabolite Identification
Get accurate quantitative information on your parent compound and simultaneously collect rich qualitative information about its metabolism with the TripleTOF 6600® or TripleTOF® 5600+ Systems with SWATH® Acquisition and MetabolitePilot™ Software. SCIEX and Hepregen have collaborated to deliver a comprehensive metabolite identification solution for drug metabolism scientists with the Hepregen HepatoPac® kits. From cell culture to interpretation, this solution provides an extensive view into in vitro data to enable researchers to generate, identify and analyze metabolites in one simple process.
This SCIEX complete solution will:
Provide quicker, accurate information on metabolites across multiple species
Accelerate your discovery process by providing metabolic soft-spot data that is invaluable to informing medicinal chemists in their research studies
Achieve better optimization of animal studies with for faster results to help reduce expenses
Give you the power to inform and drive your drug development programs
Efficient data mining, cross species comparisons and retrospective analysis without any additional requirement for re-injection
Multiple powerful data processing algorithms to perform both non-targeted and targeted processing in paralle
An integrated and efficient easy to use workspace that displays all relevant information making the work of identifying and confirming metabolites
Figure 1. Intuitive MetabolitePilot™ workspace for metabolite identification workflows on accurate mass TripleTOF® systems. Access the ‘Batch Workspace’ where multiple project results can be processed together, the ‘Results Workspace’ which includes an integrated fragment interpretation tool, or the ‘Correlation Workspace’ where you can get a simple, clear view of kinetic trends and relative concentrations of multiple samples.