AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.
Antibody drug conjugates (ADCs) combine the unique targeting capabilities of monoclonal antibodies with cytotoxic drug loads, and represent one of the fastest growing segments of the biotherapeutic pipeline. Due to their inherent heterogeneity and high molecular weight, ADCs represent a huge challenge in drug discovery and development.
Analytical scientists have the complex and crucial task of accurately determining the amount of cytotoxic drug that is present on the ADC molecule. The need to understand the changes in the payload distribution over time and ensure the drug load is consistent and comparable from lot-to-lot, make it a very involved process and accuracy is paramount.
For routine intact or subunit ADC analysis, SCIEX LC-MS solutions simplify and accelerate your workflows and provides an automated and accurate view of drug load and drug-to-antibody ratio (DAR). When it comes to ADC analysis in complex matrices, our solutions deliver advanced sensitivity and separation to make DAR determination more accurate and straightforward.
SCIEX LC-MS solutions push the boundaries in intact ADC characterization. Powerful and automated data processing gives you the utmost confidence in your analysis of drug load, while ensuring the characterization process doesn’t create pipeline bottlenecks. Now you can reduce sample preparation and complicated separations, and eliminate time consuming manual calculations.
For those doing standardized analyses, the X500B QTOF System is simple, easy-to-use and robust, making it accessible for many users to quickly and accurately analyze ADC samples. More complex ADC characterization demands the additional sensitivity of the TripleTOF 6600 or orthogonal selectivity strategies, such as SelexION DMS.
For research use only. Not for use in diagnostic procedures.
High quality intact mass and subunit data for large ADC molecules is possible on SCIEX QTOF systems. See both major and minor drug forms to ensure you can calculate an accurate drug-to-antibody ratio for the biotherapeutic.
Simplified protein deconvolution and lightning fast drug-to-antibody ratio (DAR) calculations in the BioPharmaView Software means you’ll spend less time performing manual calculations. You can quickly process samples in high volume using the batch processing mode, and see drug load across samples with intuitive visualization tools and notifications to quickly determine comparability.
If your ADC is in a complex matrix, combining the highly sensitive TripleTOF® 6600 with SelexION® Differential Mobility Technology reduces data complexity, to give you a clearer picture of your ADC by separating the biotherapeutic from other background components. The SelexION device can be tuned to allow the passage of the ADC therapeutic while filtering away other smaller coeluting proteins and background matrix components.
When doing ADC analysis in complex matrices, the overall sensitivity of the system and your ability to perform up front separation of the ADC from other sample components become very important. The high performance, advanced TripleTOF 6600 System delivers high sensitivity, coupled with the SelexION Differential Mobility Technology to reduce data complexity.
Giving you the ultimate confidence for drug metabolism and catabolism studies with high sensitivity and wide linear dynamic range of >5-orders
Automated ADC data processing delivers fast and accurate drug-to-antibody ratio calculations in the analysis of drug load
If you manage routine ADC characterization and DAR determination, the X500B QTOF System paired with BioPharmaView™ Software provides faster and simpler tools for setup and analysis. You can easily see drug load across multiple samples with automated DAR calculations.
Delivers high accuracy, reliability and repeatability across thousands of injections, with maximum uptime
Accurate mass analysis with the compact and streamlined QTOF System and the renowned Turbo V™ ionization source
Intuitive point-and-click acquisition software makes it easier than ever to set-up and run your methods
Technical Note: Intact Analysis of Antibody Drug Conjugates
Technical Note: Glycyosylated and Deglycosylated Subunit Analysis of Antibody Drug Conjugates
Method Flyer: Intact Antibody Drug Conjugate Analysis Method
Solutions Guide: Intact and Subunit Mass Analysis
Technical Note: Analysis of ADCs by TripleTOF and SelexION DMS
Poster: Optimizing Chromatography for High Resolution Antibody Drug Conjugate DAR Characterization
Blog: Fast and Accurate DAR Analysis Without the Complexity
Solutions Guide: Intact Mass Analysis
Technical Note: Automating DAR Calculations for Greater Efficiency in Analyzing ADCs
Webinar: Optimizing Chromatography for High Resolution Antibody Drug Conjugate DAR Characterization
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