CESI-MS is used by BioPharma Leaders for the analysis of intact biologics such as monoclonal antibodies (mAbs) and Erythropoietin (EPO), peptide mapping, disulfide mapping, identification of post-translational modifications, and antibody drug conjugates (ADCs).
Intact Biologics – CESI-MS is able to quickly and accurately characterize intact proteins for product quality, purity, and safety assessments.
Peptide Mapping – CESI-MS has exceptional separation efficiency for peptide -- (especially the very short or very long, or highly charged peptides) and glycopeptide mapping of mAbs, differentiating between Asp and IsoAsp deamidation and other PTMs – with the ability to provide 100% coverage from a single enzyme digestion in a single analysis.
Identification of Post-Translational Modifications – CESI-MS readily separates and increases sensitivity of deamidated, glycosylated, citrullinated, phosphorylated, and other PTMs.
Antibody Drug Conjugates – CESI-MS allows for the separation of various ADCs with confident analysis of drug antibody ratio.
Multi-level characterization of mAbs and other biologics
Dive deeper into your intact biotherapeutic analysis
Click to Enlarge Charge Heterogeneity Separation of a Stressed IgG, showing the deconvoluted mass spectrum (top)
Analyzing three ADC characteristics by CESI-MS
Intact ADC separation and DAR analysis
Click to Enlarge Intact Antibody Drug Conjugate (ADC) analysis showing separated protein peaks at Drug Antibody Ratios (DAR) of 1, 2, 3, and 4.