AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.

cIEF and CZE Charge Heterogeneity Analysis of Biologics

Identity/Charge Heterogeneity – Remarkably Fast, Accurate and Easy to Use

cIEF and CZE Charge Heterogeneity Analysis of Biologics

When working with a mAb, you need to know the different charge variants to determine identity and stability. You also need the utmost confidence in your results, as the success of your biologic can depend on the accuracy of your results.

Whether you have several methods to develop, samples to QC with limited resources or labs around the world that all need to use the same methods, you need a solution that you can trust. You need to develop charge heterogeneity methods with high resolution and precision that are fast and easy to use, as well as easily transferable across the globe.

The SCIEX PA 800 Plus allows you to confidently assess charge heterogeneity with ultra-high resolution (CIEF) or simplify your sample preparation (CZE). The PA 800 Plus simplifies your workflows and streamlines your methods so you can improve lab productivity and performance. The system also provides you with the raw data required for regulatory acceptance.

Get Proven, Fast Accurate Results with CZE

Labs that need to analyze mAbs for identity and stability can rapidly obtain high resolution charge heterogeneity information with simple sample preparation, using CZE. You can also develop methods with high resolution and precision, that are fast and easy to use, as well as easily transferable across the globe.

Ultra-high Resolution with cIEF

For maximum sensitivity, ultra-high-resolution capillary isoelectric focusing (cIEF) is capable of achieving separation between isoforms as closely related as 0.03 pI units. The SCIEX cIEF workflow on the PA 800 Plus System has proven robustness and portability. Universal or platform methods can also be created – significantly decreasing method development efforts, simplifying workflows with a single method for molecules across a wide pI range.

RUO-MKT-18-6288-A
For research use only. Not for use in diagnostic procedures.

Resources

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Technical Note
Identification of System Parameters Critical for High-Performance cIEF

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Technical Note
High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

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Technical Note
A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range

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Technical Note
Achieving Robust cIEF Analysis of Monoclonal Antibodies While Increasing Capillary Run-Life and Maintaining High Resolution

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Webinar
Intercompany Study to Evaluate the Robustness of CZE Technology for the Heterogeneity Analysis of mAbs

Watch Webinar

Webinar
MAb charge heterogeneity analysis - A case Study from Merck Sharp & Dohme

Watch Webinar

Webinar
MAb charge heterogeneity analysis - A Test Method Fit for QC Testing

Watch Webinar

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Quick and Easy Charge Heterogeneity Analysis by CZE

Capillary zone electrophoresis (CZE) results in faster separation compared to liquid chromatography, reduced sample preparation complexity, and minimal required optimization – all with high reproducibility.

Maximize Resolution with cIEF

For maximum resolution, capillary isoelectric focusing (cIEF) is capable of achieving separation between isoforms as closely related as 0.03 pI units.

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Charge Heterogeneity Analysis

Watch Video

Which is Right for Your Lab?

Watch the Webinar: Charge Heterogeneity Analysis of Biologics by High Resolution cIEF and High Throughput CZE Methods

Video Length 57:03

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Experiencing Difficult mAbs?

Methods for Particular pH Ranges Available

Reduce Method Optimization

Broad pH coverage (3-10) in a single run means you run fewer batches

Trust Your Results

Be confident in your results with reproducible, highly accurate pI determination

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Easy Sample Prep

Save time with fast, simple sample preparation.

Faster Separation

Screen charge variants in about 10 minutes.

Simplified Methods

Use the same method over a wide range of pI.

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PA 800 Plus

PA 800 Plus

Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.

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Advanced cIEF Starter Kit

Advanced cIEF Starter Kit

The Advanced cIEF Starter Kit helps you get the highest level of precision for pI determination and isoform quantitation of your sample.

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pI Marker Kit

pI Marker Kit

pI Marker Kit features synthetic peptides for high resolution and quantitative reproducibility in calculating protein pI.

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PA 800 Plus

PA 800 Plus

Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.

Read More
EZ-CE Cartridge

EZ-CE Cartridge

With an inventory of just the EZ-CE cartridge you can run CE-SDS, Fast Glycan, and Rapid Charge Variant analyses.

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Expert Support

Expert Support

Access comprehensive services and support from recognized industry leaders in CZE.

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AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.