cIEF and CZE Charge Heterogeneity Analysis of Biologics
Advanced capillary isoelectric focusing (cIEF) and hi-speed capillary zone electrophoresis (CZE) for charge heterogeneity analysis
The PA 800 Plus Pharmaceutical Analysis System (PA 800 Plus) determines protein charge isoform heterogeneity using high resolution capillary isoelectric focusing and zone electrophoresis. These assays allow you to confidently assess protein stability with ultra-high resolution (CIEF) or simplify your sample preparation (CZE).
Protein stability can be tightly linked to the effect of environmental conditions on charge heterogeneity. The PA 800 Plus delivers the highest resolution charge heterogeneity capillary isoelectric focusing (cIEF) workflow solution.
Solutions Guide: Automated and Quantitative Analysis of Biologics using the PA 800 Plus
Technical Note: High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10
Technical Note: PA 800 plus cIEF Application Guide
Webinar: MAb charge heterogeneity analysis by CZE
The SCIEX cIEF workflow on the PA 800 Plus capillary electrophoresis system has been proven robust and portable. Universal or platform methods can also be created – significantly decreasing method development efforts, simplifying workflows with a single method for molecules across a wide pI range.
To provide you with greater sensitivity of analysis, ultra-high resolution cIEF is capable of achieving separation between isoforms as closely related as 0.03 pI units.
Alternatively you can obtain high resolution charge heterogeneity information more rapidly with simplified sample preparation, using Capillary Zone Electrophoresis (CZE).
High Throughput Charge Heterogeneity Made Easy
When determining charge heterogeneity on the PA 800 Plus, capillary zone electrophoresis (CZE) - a simple buffer CE technique - results in faster separation compared to liquid chromatography and other capillary electrophoresis methods. CZE offers you many other advantages, including reduced sample preparation complexity and minimal required optimization. As a result, CZE is being adopted by a growing number of organizations.
CZE separations can be performed with high reproducibility, ideal for multi-user, multi-instrument organizations
The Right Reagents For Your cIEF Analysis
Our cIEF Peptide Marker Kit (part number AS8481) features synthetic peptides. The combination of the advanced cIEF methods, synthetic pI markers and the PA 800 Plus system results in cIEF separations with the highest level of precision for pI determination and isoform quantitation of your sample. Try the Advanced cIEF Starter Kit (part number A80976), it contains most everything you need.