Protein Methods Development
With capillary zone electrophoresis (CZE) proteins are separated by their electrophoretic mobility, a function of the charge and mass of a protein at a given pH. When using electrolytes with pH > 4.0, the surface of bare-fused silica becomes negatively charged, inducing both electroosmotic flow (EOF) and the adsorption of proteins that possess a net positive charge. The Protein Methods Development kit, contains coated capillaries, buffers, standards and EOF markers to allow you to optimize a separation method for the analysis of a broad spectrum of proteins. The use of this Neutral capillary surface serves to significantly reduce EOF and to prevent adsorption of proteins to the capillary surface. These Neutral capillaries have a two layer coating, with the first layer a bonded phase serving to deactivate the silanol groups and a second hydrophilic layer that protects against hydrophobic interactions improving the overall efficiency and resolution of proteins separated by CZE. This chemistry is designed to be used with the PA 800 Plus Pharmaceutical Analysis System UV detector only - the higher energy generated by the PDA detector may serve to degrade the capillary surface.