A - In data-independent acquisition strategies like SWATH™ Acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition, high resolution extraction ion chromatograms for specific fragment ions of the peptides of interest and these are integrated for quantitation. The information for extraction is stored in an spectral ion library and this contains all the proteins and peptides that you are interested in for the specific proteome you are studying.
What does this enable?
- Data processing post-acquisition: generate fragment ion XICs at high resolution for quantitation with confirmation of identity
- Quantitation and confirmation of everything in the sample
- Digital record of all detectable peptides in your sample
- Single method for acquiring all your data