Recently, you may have read some of my posts discussing SWATH® Acquisition technology, and I wish to continue that trend, as it applies to a forensic poster presented at the American Academy of Forensic Sciences Annual Scientific Meeting in New Orleans, Louisiana, and has a profound impact on the forensic community.
Upon attending this meeting, attendees had the chance to understand two powerful data acquisition modes as presented in a poster talk, “SWATH vs. Information Dependent Acquisition (IDA) with Application to Broad Based Screening.” The purpose of this discussion involved applying these technologies to laboratory screening for drugs of abuse by liquid chromatography time-of-flight mass spectrometry (LC-TOF) and seeing which strategy came out on top. Both acquisition modes, as we know, generate accurate mass data that can be searched against accurate mass extracted ion chromatogram (XIC) lists and accurate mass spectral library databases. However one of them results in a more accurate analysis which you find out more about when you read this study.
Discover which technique finds all detectable data and turned out zero false positives with just a single false negative collected from 139 blood samples at an electronic dance festival in Miami for which 40 were chosen at random. From the acquired data, this study shows one acquisition method is preferable over another based on more reliable positive screening results.