Course Catalog

This course will introduce you to the Agilent Lab Advisor Software and give you an overview of the software and its functionality.

After taking this course, you should be able to:
- Add and connect a system
- Configure your system
- Update the firmware
- View logs, messages and results for your system
- Perform Service and Diagnostics procedures
- Control your system using Lab Advisor software
- View and update EMF counters
- Create reports

Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 3bb79371-5fb6-4514-b20e-affc04909247

This course covers an overview of the AutoPeak integration algorithm available in the Analytics workspace of SCIEX OS software.

After taking this course, you should be able to:
- understand the theory of the AutoPeak integration algorithm
- create a processing method using AutoPeak
- describe basic and advanced AutoPeak integration parameters
- explain how saturation correction is applied
- create results and review data using AutoPeak

Duration: 15 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 497b329c-7d1d-4005-99bd-4d8fccaf2132

This course will introduce you to the MPX^TM 2.0 High Throughput System and how automated carryover monitoring works.

After taking this course, you should be able to:
- Describe how multiplexing works
- Explain the automated carryover monitoring process
- Create acquisition and quantitation methods
- Create and submit a batch
- Review the batch in the MPX software

Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: caa2be0a-4fb3-47b1-95cc-2867f6d59111

This course demonstrates how to change the roughing pump oil for your SCIEX mass spectrometer, and is applicable for the following systems:
- Triple quadrupole and QTRAP® systems (RUO and MD)
- X500 QTOF series systems
- TripleTOF® systems

It will cover the following topics:
- Overview of why you should change the roughing pump oil and when to change it
- Tools and materials needed
- Steps involved in the oil change process

Duration: 10 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 4b91fac0-b3be-4b5e-b6a6-54c2063062d1

This eLearning course will cover several examples of how to create flagging rules and custom columns in SCIEX OS.

After completing this course, you should be able to:

• Create custom columns
• Create flagging rules using default and custom columns
• Perform data review using flagging rules and custom columns
• Import and export flagging rules into a method.
  

Chapters: 1
Duration: 20 mins
Learner Level: Intermediate to Advanced
Certificate Type: Completion
PACE Accreditation: None
Course ID: 210a7b13-a8d9-47c0-9bc6-321a8d608b13

This course is comprised of 4 chapters that cover the data acquisition and processing workflow on the Lipidyzer ^TM Platform. It is intended that you view each of these chapters in the following sequence shown. You will achieve a certificate once you have successfully completed all four chapters of this course.

Chapter 1: Performance Tests teaches you how to run the performance tests for the Lipidyzer Platform in order to ensure that settings for each lipid class are correct and to verify that the instrument is performing to specification.
Chapter 2: Batch Generation teaches you how to register an internal kit and then create a Lipidyzer Workflow.
Chapter 3: Sample Extraction tells you how to create an internal standard superpool mix and complete a successful Bligh & Dyer Lipid extraction.  
Chapter 4: Results shows you how to view and report your results from your samples using the projects and batches functions in the Lipidomics software. 

Chapters: Four (4)
Duration: 10-20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 8a5d9a1a-982e-49a1-bb70-bd3e188a56f2

This eLearning course will demonstrate how to remove the OptiFlow™ interface from your TripleTOF® system and switch between nanoflow and microflow or high flow.

After completing this course, you should be able to:
- Remove the nano ion source and install a high or microflow ion source
- Remove the OptiFlow interface and install the high flow curtain plate
- Install the microflow electrode in the probe for the Turbo V™ ion source

Chapters: 1
Duration: 4 mins
Learner Level: Basic
Certificate Type: None
PACE Accreditation: None

After taking this training course, you will be able to perform basic operations in PeakView® Software and use some of the more advanced functions such as FormulaFinder and Fragment Pane for the Met ID workflow.

This course is intended to cover the theory of Differential Mobility Separation (DMS) Technology and how to use the SelexION® device with your mass spectrometer.

After taking this course, you should be able to:
- Understand the theory of DMS and DMS method parameters
- Install the SelexION device and equilibrate the system before use
- Optimize DMS method parameters for your analytes
- Perform basic maintenance tasks

Refer to the Resources section on the left to view additional documents:
- Workflows for Method Development and Routine use
- Cleaning Procedures
- Installation and Removal Procedures

Duration: 25 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 01495374-15b5-4609-b824-d8dca8cf2534

This eLearning course will give an overview of the Echo® MS System. It will cover the following topics:

• Components and theory of the Echo MS System
• How to configure the Echo MS System using SCIEX OS
• Creating MS, AE and processing methods and batches
• Using Direct Device Control
• Maintenance tasks

Chapters: 1
Duration: 15 mins
Certificate Type: Completion
PACE Accreditation: None
Course ID: ff42eecc-68c8-4c60-8476-2900e5304fe1

This eLearning series covers the OptiFlow Quant Solution for microLC. It applies to selected triple quadrupole, QTRAP® and TripleTOF® systems coupled with an OptiFlow® Turbo V ion source and the M5 MicroLC system. The series consists of a two part course:
System Overview
System Setup and Use

After completing this course, you should be able to:
- Describe the different components in the OptiFlow Quant Solution
- Setup and use the OptiFlow Turbo V ion source
- Setup the system for the M5 MicroLC system
- Describe the trap-elute workflow
- Define mobile phases, purge and flush the M5 MicroLC system
- Create autosampler methods using the Analyst Device Driver
- Create acquisition methods for direct injection and trap-elute experiments
- Understand best practices and maintenance of the M5 MicroLC system

Chapters: 2
Duration: 10-30 mins (for each chapter)
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 688bf733-b1e8-47fb-9133-01bb4454cce9

During this 30 min presentation, you will learn about the fundamental challenge of lipidomics analysis, namely isobaric interference, what SelexION® Technology is and how it works, the development of DMS as a tool for lipid analysis by mass spectrometry and finally see how SelexION® Technology has been used to solve difficult analytical problems in lipidomics.

The overall objective of this eLearning module is to describe in detail the different aspects of the SelexION® Technology device, including the hardware components and parameter settings. During this 30 min presentation, you will learn about the procedures to place the DMS cell onto the mass spectrometer and equilibrate the instrument. Additionally, the different parameter settings that control the function of the SelexION® Technology device will be introduced and discussed in terms of how they affect experimental results.

The overall objective of this eLearning module is to describe how to use the SelexION® Technology device during qualitative analysis and to improve the qualitative characterization of lipids. During this 40 min presentation, you will learn how to develop qualitative analytical assays using the SelexION® Technology device on QTRAP® or Triple Quadrupole Systems or Triple TOF® Instruments. The sections in this module include COV optimization strategies on SCIEX instrument platforms, example data from qualitative lipid analysis and online resources.

The overall objective of this eLearning module is to describe how to use the SelexION® device during quantitative analysis of lipids. The use of SelexION® Technology (differential ion mobility spectrometry, or DMS) in the study of lipidomics greatly improves the quantitative analysis of lipids by lowering the chromatographic baseline (i.e., noise) and minimizing isobaric interference. In this 20 min presentation, the steps needed to develop quantitative assays using DMS will be described on SCIEX Triple™ Quadrupole Instruments/QTRAP® System.

The overall objective of this eLearning module is to describe routine maintenance of the SelexION® Technology device during the analysis of lipids. Regular cleaning of the DMS cell is suggested to maintain its robustness and performance. In this 20 min presentation, the procedures to clean the hardware are presented, and a troubleshooting guide is provided.

This module will inform you how to perform basic maintenance for the Lipidyzer Platform, and includes some FAQs.

After taking this module you should be able to….
   - Install all hardware parts of the SelexION® device
  - Clean and reassemble the DMS cell
  - Check that the direct infusion spray is stable

Chapters: One (1)
Duration: 10 minutes
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: c910c531-d10f-47e0-8dd2-9f9bb3176d16

This eLearning course focuses on data processing using the non-targeted screening workflow in the Analytics workspace of SCIEX OS software. This is applicable to all SCIEX instruments (excluding MD instruments).  

After completing this course, you should be able to:
- Describe the non-targeted workflow
- Understand how library searching and formula finding can be used in data review
- Create a processing method
- Create results
- Perform data review
- Export data and create a report

Chapters: 1
Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 17cf4220-b02c-4730-9f9c-e4adde192f67

Upon completion of this training module, participants will have met the following objectives: Learn the SCIEX Protein/Peptide Identification Workflow Understand the concepts to build an Information Dependent Acquisition (IDA) method using Analyst® Software Evaluate IDA data using PeakView® Software And finally, understand the process of using ProteinPilot™ Software to search and review data

This course focuses on qualitative data processing using the Quantitation and Targeted Identification workflow in SCIEX OS software. This course applies to all SCIEX instruments (excluding MD instruments). 

After completing this course, you should be able to:
- Describe the quantitation and targeted identification workflow
- Understand the different types of library searches
- Create a processing method
- Create results
- Perform qualitative data review using the results table and peak review pane
- Export and report results

Chapters: 1
Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 6341f93c-6b76-482f-9239-1b569de9b9cc

This eLearning series focuses on quantitative data processing using the Analytics workspace in SCIEX OS software for all SCIEX instruments (excluding MD instruments). This entire eLearning series consists of 3 modules : Algorithms and flagging, Create method and results, and Review and report results. 

After completing this eLearning series of modules, you should be able to:
- Understand the different types of integration algorithms and their parameters
- Explain the pre-defined flagging rules for concentration, accuracy, integration and ion ratios
- Create a processing method
- Create results
- Perform data review
- Export and report results

Chapters: 3
Duration: 10-30 mins (for each module)
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None

This series of short maintenance videos focuses on the SCIEX M5 MicroLC System, and cover how to perform simple instrument maintenance tasks. 
- Putting PAL Virtual Terminal into Extended User Mode
- Replace the Valve Stator and Rotor Seal
- Replace the Sample Loop 
- Prime the Autosampler Wash Pumps
- Replace The Syringe Needle
- Replace the Syringe Barrel and Plunger
- Measure the Flow Rate
- Calibrate the Flow Meters 
- Re-initialize Pressure Transducers

Chapters: 9
Duration: 1-6 mins (for each chapter)
Learner Level: Basic
Certificate Type: None
PACE Accreditation: None
Course ID: bb0863fe-9ecd-4c35-859c-2089844e2be9

This series of short maintenance videos focuses on the OptiFlow® Turbo V Source, and covers how to perform simple instrument maintenance tasks. 
- Remove and Reinstall the Probe and Electrode
- Remove the Ion Source

Duration: 2-5 mins per video
Learner Level: Basic
Certificate Type: None
PACE Accreditation: None
Course ID: 02130b7e-c8cd-493e-8d23-b4de070dd5ba

This series of short maintenance videos focuses on the Jasper^TM HPLC System, and cover how to perform simple instrument maintenance tasks. 
- Clean Up the Leaks in the Column Oven
- Clean the Pump Leak Tray
- Remove Autosampler Clear Acrylic Panel (Panel F)
- Clean the Rinsing Port & Cover and Replace Rinsing Port Cap
- Rinse Needle and Sample loop
- Remove Condensation
- Replace the Fuses
- Clean Check Valves
- Replace the Needle Seal
- Replace Sample Loop

Duration: 1-4 mins per video
Learner Level: Basic
Certificate Type: None
PACE Accreditation: None
Course ID: f477ed6d-355c-4397-aa96-5977da21faa5

This course covers how to acquire and process SWATH ^® data using MetabolitePilot ^TM Software 2.0 with TOF instruments controlled either by the Analyst ^® TF or SCIEX OS software.

After taking this course, you are now able to:
- Describe SWATH acquisition and its workflow
- Create SWATH acquisition methods
- Build a batch and acquire SWATH data
- Process, review and interpret SWATH data using MetabolitePilot for small molecule, peptide and ADC workflows

Duration: 30 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 4f3e325c-3655-4538-bd10-62e0de6ccb24

This course covers how to use MS Tune in SCIEX OS for SCIEX Triple Quad and QTRAP MS/MS Systems.  In this course, we will review the following topics:
- Configuring devices in SCIEX OS
- Tuning solutions
- The Tuning Instrument data file
- How to verify and tune your SCIEX system performance using SCIEX OS

Chapters: 1
Duration: 30 mins 
Learner Level: Beginner to Intermediate
Certificate Type: Completion
PACE Accreditation: None

This course covers how to create LC and MS methods for SCIEX Triple Quad and QTRAP Systems using SCIEX OS. It includes the following topics:
- Creation of an MS method
- Advanced MS method parameters
- Advanced features for an MS method such as ionization scheduling, multiple experiments, experiment scheduling and default settings
- Creation of an MS method using the Scheduled MRM algorithm
- Creation of an IDA MS method
- Conversion of an Analyst method to a SCIEX OS MS method
- Creation of an LC method

Chapters: 1
Duration: 20 mins 
Learner Level: Basic to INtermeditae
Certificate Type: Completion
PACE Accreditation: None

This eLearning course will cover the security and audit trail functionality in SCIEX OS. 

After completing this course, you should be able to:

• Understand how SCIEX OS works together with Windows to control and audit system access
• Configure users and their access to SCIEX OS functionality
• Set up audit map templates for auditing of project and workstation events
• Review audited events using the Audit Trail workspace
• Use the Event Log workspace to view unaudited system events 

Chapters: 1
Duration: 20 mins 
Learner Level: Beginner to Intermediate
Certificate Type: Completion
PACE Accreditation: None

This eLearning course will show you how to assess the performance of your TripleTOF® system for microLC using the SWATH® Acquisition Performance Kit. For data processing, you will need to use PeakView® Software, SWATH Acquisition MicroApp, SWATH Replicate Analysis template and ProteinPilot^TM Software. 

After completing this course, you should be able to:
- Perform a direct infusion test using PepCalMix
- Perform a system performance test using K562 Extract and PepCalMix
- Perform autocalibration using PepCalMix
- Check the performance of SWATH acquisition
- Assess the protein identification performance

Chapters: 1
Duration: 30 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 8205f5f4-9f97-4903-8976-900888993710

This eLearning course will show you how to assess the performance of your TripleTOF® system for nanoLC using the SWATH® Acquisition Performance Kit. For data processing, you will need to use PeakView® Software, SWATH Acquisition MicroApp, SWATH Replicate Analysis template and ProteinPilot ^TM Software. 

After completing this course, you should be able to:
- Perform a direct infusion test using PepCalMix
- Perform a system performance test using K562 Extract and PepCalMix
- Perform autocalibration using PepCalMix
- Check the performance of SWATH acquisition
- Assess the protein identification performance

Chapters: 1
Duration: 30 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 99057a24-4242-41fb-94f3-6588fa629e49

This course is designed to enable you to troubleshoot system performance issues for the TripleTOF® 6600 and 6600+ System with proteomics applications. 

After completing this course, you should be able to:
Describe common issues that occur for proteomics applications with the TripleTOF 6600 and 6600+ System
Understand how to use the Tuning Solution and SWATH® Acquisition Performance Kit to diagnose issues
Identify Q0 or IQ0 front end contamination of your system
Recognize issues that could be caused by the LC components

Chapters: 1
Duration: 30 mins 
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: e2abd48c-8827-45ab-964f-df934b10b162

After taking this training course, you should be able to: Understand how to manually determine possible peptide sequences from a proteomics IDA datafile using the ‘Manual Sequence’ function, and link an experimental spectrum to a theoretical peptide sequence; Take peptide data and process with the “LC/MS Peptide reconstruct” function to deconvolve spectral peaks and produce a peptide map; Utilize the ‘Digest Protein’ function to obtain all theoretical peptide sequences from a protein of interest, and how to link this back to peptide mapping data; Use the ‘Manual Reconstruct’ function for both peptide and protein data to identify the MW and potential charge states of a target; Obtain a deconvolved, zero charge mass graph for an intact protein target using the ‘Protein Reconstruct’ function