mTRAQ® Reagents

For research use only. Not for use in diagnostic procedures.

The mTRAQ Reagents are amine-specific, stable-isotope-labeled reagents, available in duplex or triplex format, designed for high-confidence protein and peptide biomarker verification using multiple reaction monitoring (MRM) analysis on QTRAP Systems and the Triple Quad LC/MS/MS Systems.

The use of this triplex chemistry will increase sample throughput by 100%.

  • Duplex or triplex mass-difference tags (non-isobaric amine tags) available
  • Established chemistry with >95% labeling efficiency
  • High-selectivity, MRM-based, relative quantitation of proteins/peptides/PTMs
  • Global Internal Standard (GIS) workflow is supported for robust, reproducible, quantitative MRM assays in biomarker verification
  • Fractionation-compatible reagent
  • Easy transition from protein discovery based on labeling with iTRAQ Reagents to verification
  • Significantly more economical approach compared to use of stable-isotope-labeled synthetic peptides for biomarker verification when moitoring larger numbers of peptides
  • Biomarker verification supported by a complete suite of software, including ProteinPilot, MRMPilot, and MultiQuant Software

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Status: Available Item status information

 

Overview

Innovative peptide-tagging chemistry: The mTRAQ® Reagents are non-isobaric, peptide-labeling reagents that enable you to label all primary amines, regardless of peptide class. The mTRAQ® Reagents are an extension of the iTRAQ® Reagent technology and are chemically identical, aside from isotope content (Figure 1). Peptides labeled with different mTRAQ® Reagents have identical retention times and ionization characteristics but different masses (0, 4, or 8 amu mass difference for triplex-labeled, arginine-containing peptides, and 0, 8, or 16 for lysine-containing peptides).  Multiple reaction monitoring (MRM) quantitation is performed using the non-isobaric, sequence-specific ions, instead of reporter ions, for higher specificity in complex mixtures.

High-confidence biomarker verification using robust methodology: Obtain robust performance with Global Internal Standard (GIS) methodology. The GIS is prepared by labeling pooled samples with mTRAQ® Reagent Δ8. This GIS is then spiked into individual samples labeled with mTRAQ® Reagent Δ0 or mTRAQ® Reagent Δ4 prior to MRM analysis (Figure 2). This internal standard process offers significant benefits such as:

  • Enables normalization and comparison across
    • Many samples
    • Entire duration of the project
    • Multiple instruments/multiple laboratories
  • Corrects for experimental inconsistencies
    • Injection inconsistencies
    • Changes in MS performance
    • Changes in LC performance
  • Most economical approach
    • Quantitation of a large number of proteins and peptides is enabled by providing a more cost-effective way to create internal standards
    • mTRAQ® Reagents Triplex will double sample throughput

Complete solution for targeted quantitative proteomics: One key advantage of being able to analyze hundreds of compounds a week is that you can quickly build a substantial database of optimization conditions. The optimized conditions for a given compound is stored by DiscoveryQuant™ Software in a networked database, which can then be used at any time, in any location, with other mass spectrometers  – a significant advantage for pharmaceutical companies in the age of globally-connected data.

Kits

 

Part Number

Kit Name/Description

4352157 Trypsin, TPCK treated, 10-Pack
A modified (TPCK treated) serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Contains calcium chloride which may improve consistency in digestion. Modified trypsin is treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. Each package contains 10 vials containing 25 μg in each vial, sufficient for 12 duplex when using 100 ug of sample.
4370285 Trypsin, TPCK treated, 8-Pack
A modified (TPCK treated) serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Modified trypsin is treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. Each package contains 8 vials containing 25 μg in each vial.
4374771 mTRAQ Reagent 10 Assay Kit (10 assays of each reagent Δ0, Δ4, and Δ8)
Contains mTRAQ Reagents Δ0, Δ4, and Δ8, sufficient for 20 mTRAQ assays. One unit of reagent supports labeling up to 100ug of protein. The kit has 10 tubes each of Δ0, Δ4, and Δ8 reagents, each containing 1unit of reagent; also includes Protocol and Quick Reference Card. Optional Multiplex Buffer kit (PN 4381664) sold separately.
4381664 iTRAQ Reagent- Multiplex Buffer Kit
Contains SDS – Denaturant, TCEP Reducing Reagent, MMTS Cysteine Blocking Reagent, TEAB Buffer and Isopropanol, enough for 2 - 8plex Multiplex Kits, 3 - 4plex Kits, and 4 - mTRAQ 10 Assay Kits.
4427698 mTRAQ Reagent Δ4 50 Unit Pack
Contains mTRAQ Reagents Δ4 for 50 mTRAQ assays. One unit of reagent supports labeling up to 100ug of protein. Pack has 1 tube each of Δ4 containing 50 units of reagent. Optional Multiplex Buffer kit (PN 4381664) sold separately.
4427700 mTRAQ Reagent Δ8 50 Unit Pack
Contains mTRAQ Reagents Δ8 for 50 mTRAQ assays. One unit of reagent supports labeling up to 100ug of protein. Pack has 1 tube each of Δ8 containing 50 units of reagent. Optional Multiplex Buffer kit (PN 4381664) sold separately.
4440015 mTRAQ Reagent Δ0 50 Unit Pack
Contains mTRAQ Reagents Δ0 for 50 mTRAQ assays. One unit of reagent supports labeling up to 100ug of protein. Pack has 1 tube each of Δ0 containing 50 units of reagent. Optional Multiplex Buffer kit (PN 4381664) sold separately.