Using triple quad mass spec systems to detect MRM transitions of compounds is a common approach to detecting chemical compounds in food. But it is actually very easy to upgrade your MRM acquisition method on a QTRAP mass spec system to not only collect your MRM transitions, but to also collect full MS/MS fragmentation data on every peak detected - enabling you to compare your acquired data to compound libraries for more confident identification of compounds that are present in your samples.
The benefits of this workflow are vast
Improve your data acquisition - make sure you're detecting every peak present in your samples.
Improve your sensitivity - enhanced MS/MS scans gives you better signal to noise for better compound detection.
Improve your selectivity - full MS/MS scanning is like detecting 4 or more MRM transitions (way better than just 2 MRM transitions) for more confidence in reporting the right results.
Figure 1. Schematic of full MS/MS, or enhanced product ion scan, collected on a QTRAP MS/MS system.
Improve your data processing - don't spend hours at the compouter processing samples or performing re-analysis to verify questionable results. The 5 click MasterView workflow and the added confidence of comparing your results to compound libraries will decrease your data processing time, increase your throughput, and decrease your sample turnaround time.
Tips and guides to collecting better data
Upgrade your MRM method to utilize the Scheduled MRM Pro algorithm (for better data quality) and to collect enhanced product ion scans (EPI scans, or full MS/MS spectra) for any MRM detected in your samples.
See the video - we processed a full batch of food samples in just a few minutes!
Tips and guides to adding compounds or creating new compound libraries
Food labs typically monitor hundreds of different chemical compounds, from pesticides to veterinary drug residues to mycotoxins and more. Creating compound libraries for all of these compounds of interest can be a huge task. But never fear, we've done much of the work for you.
Learn more about the compound libraries available from SCIEX - all containing full MS/MS fragmentation patterns from REAL DATA collected on SCIEX mass spec systems (not calculated or theoretical fragments). But, if you want to add compounds or customize compound libraries for your specific applications, we have tools to help you do that as well.