SWATH® Acquisition: On the Forefront of HIV-1 Research
A research team at the University of Nebraska Medical Center (UNMC) is using SWATH® to advance a host-oriented antiviral strategy that targets the biomolecules required for viral replication.
Using SWATH® for quantitative proteomics together with bioinformatic analyses to identify host proteins, the team quantified the expression of 3,608 proteins in uninfected and HIV-1-infected monocyte-derived microphages.
Of these, they found that 420 were significantly altered upon HIV-1 infection, and the findings highlighted a novel set of proteins and processes that are involved in the host response to HIV-1 infection.
Dr. Ragu Ramanathan, Associate Research Fellow at Pfi zer Research and Worldwide Development, found in his work that quantitative results with SWATH® were comparable to those obtained using triple quad-based MRM methods. He noted that SWATH® provided better MS/MS spectra with less background interference for higher mass range metabolites. In addition, Dr. Ramanathan discovered that sample complexities were minimized by using multiple Q1 windows.
Comparing Quantitation Techniques
"Using SWATH® is a bonus for us because it preserves a lot of information on the sample - not only metabolite information, but biomarker information as well," he said. "Whenever needed, you can just go back and data mine the information."