Aav capsid purity

AAV capsid purity: the control strategy & the evaluation of CE-SDS with LIF detection

Plasmid DNA is an important part of the biopharmaceutical industry. It is used as a gene-delivery vehicle for DNA vaccination and as a key intermediate for processes like viral particle production for gene therapy and ex-vivo protein synthesis. In order to manufacture injectable plasmid, federal regulations require purity testing on these products. Unfortunately, traditional agarose gel has limitations on quantitation.

In this webinar, we present a method for plasmid analysis by capillary electrophoresis with Laser Induced Fluorescence detection with the use of a common dye. CE-LIF provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA isoforms.

You will:

  • Review current strategies and gaps for determining capsid protein purity of AAV gene therapy candidates
  • Learn novel labeling approaches to enable CE-LIF detection of AAV capsid proteins
  • Discvoer a QC-friendly method for routine monitoring of capsid purity compared with other CE-based analysis

Who should attend:

  • Lab scientists or managers developing gene therapies

Speaker

RUO-MKT-18-11555-A

Watch on demand