AB Sciex is doing business as SCIEX. © 2010-2018 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.

 

Using Microflow LC–MS for Top-Down Sequencing and Bottom-Up
Peptide Mapping

On-Demand

This webinar will describe the use of microflow LC-MS for top-down protein sequencing and bottom-up peptide mapping. This case study focuses on a key protein, DJ-1, involved in the development of Parkinson's disease. Proteins interacting with DJ-1 were characterized and mutations in DJ-1 were evaluated for their neuroprotective effect. Many binding partners were identified and quantified by bottom-up sequencing (AP-SWATH®). The majority were associated with cellular response to oxidative stress. This presentation highlights the advantages of the microflow LC-MS approach compared with nano flow or high flow LC-MS.

Key Learning Objectives

  • Understand how microflow LC-MS improves robustness compared with nanoflow LC-MS
  • Discover the throughput and detection limits of microflow LC-MS compared with traditional flow LC-MS
  • Learn how this approach may be used to characterise protein mutations and interactions

Who Should Attend

  • Scientists in academia, government and the pharmaceutical industry involved in proteomics research
  • Scientists using nanoflow LC/MS
  • R&D, analytical development and Quality Control laboratory managers and staff at biopharmaceutical companies
On-Demand Bruno Manadas, PhD
Center for Neuroscience and Cell Biology,
University of Coimbra, Portugal

RUO-MKT-11-5126-A

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AB Sciex is doing business as SCIEX. © 2010-2018 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.