For Research Use Only. Not for use in Diagnostic Procedures. Trademarks and/or registered trademarks mentioned herein are the property of AB Sciex Pte. Ltd., or their respective owners, in the United States and/or certain other countries. For information on availability, please contact your local representative.
AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. ICAT™ is a trademark of the University of Washington and is exclusively licensed to AB Sciex Pte. Ltd.
© 2019 DH Tech. Dev. Pte. Ltd.

Using Microflow LC–MS for Top-Down Sequencing and Bottom-Up
Peptide Mapping

On-Demand

This webinar will describe the use of microflow LC-MS for top-down protein sequencing and bottom-up peptide mapping. This case study focuses on a key protein, DJ-1, involved in the development of Parkinson's disease. Proteins interacting with DJ-1 were characterized and mutations in DJ-1 were evaluated for their neuroprotective effect. Many binding partners were identified and quantified by bottom-up sequencing (AP-SWATH®). The majority were associated with cellular response to oxidative stress. This presentation highlights the advantages of the microflow LC-MS approach compared with nano flow or high flow LC-MS.

Key Learning Objectives

  • Understand how microflow LC-MS improves robustness compared with nanoflow LC-MS
  • Discover the throughput and detection limits of microflow LC-MS compared with traditional flow LC-MS
  • Learn how this approach may be used to characterise protein mutations and interactions

Who Should Attend

  • Scientists in academia, government and the pharmaceutical industry involved in proteomics research
  • Scientists using nanoflow LC/MS
  • R&D, analytical development and Quality Control laboratory managers and staff at biopharmaceutical companies
On-Demand Bruno Manadas, PhD
Center for Neuroscience and Cell Biology,
University of Coimbra, Portugal

RUO-MKT-11-5126-A

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For Research Use Only. Not for use in Diagnostic Procedures. Trademarks and/or registered trademarks mentioned herein are the property of AB Sciex Pte. Ltd., or their respective owners, in the United States and/or certain other countries. For information on availability, please contact your local representative.
AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. ICAT™ is a trademark of the University of Washington and is exclusively licensed to AB Sciex Pte. Ltd.
© 2019 DH Tech. Dev. Pte. Ltd.