The SCIEX clinical diagnostic portfolio is For In Vitro Diagnostic Use. Rx Only. Product(s) not available in all countries. For information on availability, please contact your local sales representative or refer to https://sciex.com/diagnostics. All other products are For Research Use Only. Not for use in Diagnostic Procedures.
Trademarks and/or registered trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners in the United States and/or certain other countries.
© 2020 DH Tech. Dev. Pte. Ltd.

Using Microflow LC–MS for Top-Down Sequencing and Bottom-Up
Peptide Mapping

On-Demand

This webinar will describe the use of microflow LC-MS for top-down protein sequencing and bottom-up peptide mapping. This case study focuses on a key protein, DJ-1, involved in the development of Parkinson's disease. Proteins interacting with DJ-1 were characterized and mutations in DJ-1 were evaluated for their neuroprotective effect. Many binding partners were identified and quantified by bottom-up sequencing (AP-SWATH®). The majority were associated with cellular response to oxidative stress. This presentation highlights the advantages of the microflow LC-MS approach compared with nano flow or high flow LC-MS.

Key Learning Objectives

  • Understand how microflow LC-MS improves robustness compared with nanoflow LC-MS
  • Discover the throughput and detection limits of microflow LC-MS compared with traditional flow LC-MS
  • Learn how this approach may be used to characterise protein mutations and interactions

Who Should Attend

  • Scientists in academia, government and the pharmaceutical industry involved in proteomics research
  • Scientists using nanoflow LC/MS
  • R&D, analytical development and Quality Control laboratory managers and staff at biopharmaceutical companies
On-Demand Bruno Manadas, PhD
Center for Neuroscience and Cell Biology,
University of Coimbra, Portugal

RUO-MKT-11-5126-A

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The SCIEX clinical diagnostic portfolio is For In Vitro Diagnostic Use. Rx Only. Product(s) not available in all countries. For information on availability, please contact your local sales representative or refer to https://sciex.com/diagnostics. All other products are For Research Use Only. Not for use in Diagnostic Procedures.
Trademarks and/or registered trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners in the United States and/or certain other countries.
© 2020 DH Tech. Dev. Pte. Ltd.