This webinar will describe the use of microflow LC-MS for top-down protein sequencing and bottom-up peptide mapping. This case study focuses on a key protein, DJ-1, involved in the development of Parkinson's disease. Proteins interacting with DJ-1 were characterized and mutations in DJ-1 were evaluated for their neuroprotective effect. Many binding partners were identified and quantified by bottom-up sequencing (AP-SWATH®). The majority were associated with cellular response to oxidative stress. This presentation highlights the advantages of the microflow LC-MS approach compared with nano flow or high flow LC-MS.
Key Learning Objectives
Understand how microflow LC-MS improves robustness compared with nanoflow LC-MS
Discover the throughput and detection limits of microflow LC-MS compared with traditional flow LC-MS
Learn how this approach may be used to characterise protein mutations and interactions
Who Should Attend
Scientists in academia, government and the pharmaceutical industry involved in proteomics research
Scientists using nanoflow LC/MS
R&D, analytical development and Quality Control laboratory managers and staff at biopharmaceutical companies
On-Demand
Bruno Manadas, PhD Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
RUO-MKT-11-5126-A
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