Rapid magnetic Bead based Sample Preparation for Automated & High Throughput N-glycan Analysis of Biotherapeutics
We will present an optimized plate based technique for glycan analysis including carbohydrate release, sugar capture, fluorophore labeling and purification in less than 4 hours. This is followed by rapid CE-LIF analysis. This novel protocol was compared to conventional glycan sample preparation strategies using glycoprotein standards (IgG, fetuin and RNase B) and features rapid processing time, high release and labeling efficiency, excellent reproducibility with the potential for easy automation.
What will you learn?
Learn how to use commercially available bead based chemistry kit for rapid glycan analysis in less than 4 hours including capture, labeling and purification.