Analyzing Intact Proteins and Protein Complexes with CESI-MS
Despite recent progress in top-down mass spectrometry (MS) most studies are performed by infusion of highly purified proteins and protein complexes. This is mainly due to the lack of high efficiency front-end separation tools that would allow for direct analysis of these molecules. Capillary electrophoresis (CE) is an established and powerful technique for intact protein analysis, particularly in the characterization of biologics both native and denaturing The integration of CE and ESI into one dynamic process, within the same device (deemed CESI) provides the means to perform highly efficient protein separation and ionization in the ultra-low nanoflow regime (~25 nL/min) simply using an open capillary tube. Thus, CESI-MS is ideally suited for top-down proteomics, native MS, and characterization levels 1 and 2 of mAbs. In this presentation we will present examples of notable intact protein and protein complex results that have been recently generated using CESI-MS.
What will you learn?
Methods to quickly obtain high-quality intact mAb spectra from minute amounts of sample
Native analysis of proteins, complexes, and protein-drug interactions using CESI-MS
How CESI-MS is being used in top-down proteomics research