Development of High Sensitivity MicroLC-MS/MS Method for Estradiol in Human Serum Without Derivatization for the Clinical Research Laboratory
There are considerable demands to accurately measure estradiol in blood at very low concentrations (< 5 pg/mL). Most current LC-MS/MS methods for estradiol require large sample volume and involve complex sample preparation with dansyl chloride derivatization. The application of microLC-MS/MS has the potential to achieve better sensitivity and to use less sample volume. In this webinar, we will present a high sensitivity MicroLC-MS/MS method for estradiol in human serum without derivatization.
Key Learning Points:
Discuss the laboratory needs and technical challenges of developing high sensitivity LC-MS/MS methods for estradiol in blood samples
How to improve sensitivity and reduce ion suppression for estradiol on MicroLC-MS/MS system
Discuss the advantages and disadvantages of using microLC system versus normal flow LC system
For research use only. Not for use in diagnostic procedures.
June 24, 2015 2:00 - 3:00 PM ET
Xin Yi, Ph.D Clinical Chemistry Fellow, Department of Pathology The University of Chicago Medical Center