Lipid Biomarker Analysis by Differential Mobility and Mass Spectrometry
The importance lipid mediators play in host immune inflammatory initiation and resolution has translated to efforts to identify and quantify lipid molecular species as clinical biomarkers of human disease. A significant challenge to lipid analysis by mass spectrometry is the high number of isobars and isomers that interfere with quantitative analysis. Additionally, matrix interference can be a significant problem in biological specimens such as urine and plasma, which also affects quantitative rigor. Recently, Differential Mobility Spectrometry (DMS) coupled to mass spectrometry has been shown to be very effective at resolving complex mixtures of lipid isomers without the need for extensive chromatography. Furthermore, DMS is also very effective at reducing matrix interference. In this presentation, the use of DMS as a tool orthogonal to chromatography to achieve better qualitative identification and quantitative measurement of several lipid biomarkers is presented, including eicosanoids and retinoic acid isomers.
Key Learning Points
Differential Mobility Spectrometry (DMS) and the power of this technique to the study of lipid biomarkers
The balance between selectivity and sensitivity in biomarker analysis
Examples where DMS has dramatically improved lipid biomarker analysis both in terms of specificity and reduction in time of analysis
February 26, 2015 11:00 a.m. PT 2:00 p.m. ET
Paul Baker, Ph.D. Global Lead Scientist - Lipidomics SCIEX