Proteomics Approach for Analysis of Crustaceans in Food Samples
Species determination and allergen detection via MRM and MRM3 techniques
HPLC-MS based proteomics have been significantly gaining in importance for food authentication and allergen analyses over the last few years. In these fields, that are today still dominated by PCR- and ELISA-based methods in most laboratories, the QTrap technology and its recent advancements allow for the development of fast and rugged multi methods that reach so far unrivalled levels of sensitivity and selectivity.
The presentation will cover the development of such a multi method for the analysis of crustacean allergen contaminations in food samples. We will introduce you to the identification of suitable marker peptides for species differentiation, followed by the development and optimization of an MRM-based multi method for these peptides. We will finish with the introduction of the new-to-market MRM3-technology and demonstrate its potential for building ultra-sensitive methods for the detection of food allergen trace contaminations.
Key learning points
Marker peptide identification and verification
MRM- and MRM3-based method development for proteomics approaches in food analysis
December 15, 2015 11:00 A.M - 12:00 P.M. CET
Robin Korte Institute of Food Chemistry University of Münster
Dr. Ashley Sage Senior Manager Food and Environmental Business EMEA, SCIEX