Add to Calendar >2018-8-26 20:30:002018-8-28 4:30:00America/New_YorkWorkshop: CYP450 Induction2-day workshop for understanding LC-MS/MS workflow for direct measurement of CYP protein levels and the data processing with MultiQuant™ software.Center of Excellence, SCIEX Gurgaon Office, 121 Udyog Vihar, Phase -4, Gurgaon, India
The Cytochrome P450 (CYP) proteins are a family of membrane bound proteins that function as a major metabolizing enzyme in the human body. Quantification of CYP induction is critical in determining the disposition, safety and efficacy of drugs. It is advantageous to measure actual protein levels when performing such studies, which gives advantage over traditional Western blot analysis as these techniques have drawbacks due to isoform specificity and speed of analysis.
CYP Induction workshop will cover:
LC-MS/MS workflow for direct measurement of CYP protein levels
Data processing with MultiQuant™ software
Processed Area ratio result computation to compare the increase in expression of each CYP450 isoform