Determination of full, partial and empty capsid ratios for adeno-associated virus (AAV) analysis using capillary electrophoresis based technology

Adeno-associated virus (AAV) is one of the most commonly used delivery vehicles in gene therapy development. However, impurities produced during the AAV manufacturing process, such as empty and partial AAV capsids, could affect the efficacy and safety of AAV products. Although there are methodologies used to determine the ratio of these empty or partially filled capsids, they also pose their own set of challenges.

This webinar demonstrates a robust method for AAV full and empty capsid analysis that uses minute amounts of sample to deliver high resolving power for the determination of empty/full capsid ratios. The results from this methodology show potential partial capsid peaks for determination of their ratios and correlate well with orthogonal approaches.

You will:

  • Learn how to distinguish between full, partial, and empty AAV capsids for multiple serotypes with an easily optimized method
  • Discover the high resolving power of separating full and empty AAVs with very small pI differences (<=0.1 pH unit)
  • Realize rapid analysis times with less than 1 hour per sample

Who should attend:

  • Lab scientists or managers working with AAV


Tingting Li

Senior Biopharma Application Development Scientist, Biologics, SCIEX

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