Quantitative Analysis of Dicamba and Related Acid Herbicides
Acidic herbicides are used to kill broadleaf weeds before and after they sprout. These compounds have a variety of uses from controlling weeds in grain crops to ensuring roadways and fence lines are aesthetically maintained. While very effective at weed control, these controversial herbicides can drift into unintended areas like neighboring soil and animal life, as well as finding its way to the water supplies.
With the EPA ruling to continue to allow the application of Dicamba to important agricultural crops, interest in this and related herbicide compounds and their degradation products has increased in the United States. Dicamba, 2,4-D, and other herbicides comprise a large portion of the widely applied chemical herbicide compounds. Quantitative determination of these and other related acid herbicides and metabolites to low levels in relevant environmental matrices represents a crucial analytical need in the environmental and agricultural testing spaces. The ability to effectively and reliably perform quantitative analysis in complex extracts of soil and plant tissues by LC-MS/MS without the need for chemical derivatization is demonstrated.
Work is presented demonstrating limits of detection for a suite of acid herbicides and select degradation products utilizing direct analysis without chemical derivitization. The SCIEX QTRAP® 6500+ system is employed for its excellent sensitivity and robustness. Use of isotopically labelled internal standards facilitate achievement of high quality quantitation data in complex soil and plant extracts. Quantitative method performance and recovery values were investigated and reported.
Key Learning Points:
Sensitivity and performance of the SCIEX QTRAP 6500+ system for these analytically challenging contaminants
Phenomenex reverse phase column chemistry for separation and retention of underivitaized acid herbicides
Detection and quantitation of herbicide degradation products
“Real- world” sample matrices analyzed for occurrence of target herbicides and degradation products, as well as for reporting method recovery
Speaker: Katherine Hyland, PhD,
Global Technical Marketing Mgr – Food and Environmental, SCIEX
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