Aav capsid purity

Analyze the genome integrity of adeno-associated viruses (AAV) using a genome sizing methodology

A recombinant adeno-associated virus (rAAV) is a small (25 nm) virus that is composed of a capsid and a single-stranded DNA genome containing the transgene along with other elements. During the process of packaging the transgene into the rAAV capsid, errors can occur, leading to the packaging of a truncated genome as well as fragments from the host cell genome and plasmid vector. Size determination of an rAAV genome offers insight into assessing the transgene integrity of the rAAV.

Traditional methods, such as denaturing agarose-gel electrophoresis and southern blot, are time consuming and provide limited precision and resolution on size determination. In this webinar, we present a genome sizing methodology for assessing AAV transgene integrity that is fast and robust. This method is also capable of separating intact and partial genomes as well as small size impurities with high resolution and repeatability.

You will:

  • Discover a genome sizing methodology for transgene integrity analysis of AAV gene therapies
  • Learn to separate intact and partial AAV genomes from small size impurities within 15 minutes
  • Discover a rapid screening workflow that allows for 30-minute analysis
  • Explore applicable methods for various AAV serotypes with easy sample prep

Who should attend:

  • Lab scientists or managers working in gene therapy



Watch on demand