On-Demand - CESI–MS Detection and Quantitation of Neuropeptides
Analysis of large intact peptides remains challenging by LC/MS, despite recent advances in column technologies. These target peptides can be overly retained, causing peak broadening or problems with carryover and absorption to surfaces. An example is the neuropeptide, pituitary adenylate cyclase-activating peptide (PACAP). The basic nature of PACAP causes it to bind to reversed-phase stationary phases.
Integrating capillary electrophoresis separation with electrospray ionization within the same device (CESI) offers the potential to selectively detect neuropeptides at the required sensitivity. In this presentation we will discuss how CESI-MS has been used to detect targeted neuropeptides at relevant levels in biological matrices. We will also show the potential of the technique for broader peptide screening analysis.
Who Should Attend:
Scientists in academia, government, and the pharmaceutical industry involved in proteomics research and peptide analysis
R&D and analytical development directors, laboratory managers, and scientists at biopharmaceutical companies and contract research labs
Academics working in peptidomics and clinical proteomics research
Key Learning Objectives:
Learn about the challenges of analyzing highly basic peptides
Understand how CESI-MS was used to detect target neuropeptides at biologically relevant levels
Discover how CESI-MS is being expanded to include other relevant peptides
Presenter: Dr. Stephen Lock
Market Development Manager, SCIEX, EMEA
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