Characterization Of Host-Cell Proteins Using Mass Spectrometry
Characterization of Host-cell Proteins Using Mass Spectrometry Enables Effective Purification Optimization
Biopharmaceuticals are produced in common mammalian cell lines and can be co-purified with a subset of the host-cell proteins (HCPs) in the cell culture supernatant. These co-purified HCPs are considered process-related impurities and can cause potential safety risks by introducing anti-HCP response in the patients.
In this webinar Dr. Li Zang of Biogen will take a deeper look at the potential impacts reported of the residual HCPs, including, lowering the biopharmaceutical protein stability and affecting the efficacy of the biopharmaceutical protein by exacerbating the symptoms.
In addition, Dr. Stephen Tate from SCIEX will discuss the benefits of LC-MS using SWATH® acquisition as a robust and sensitive method for low-level HCP analysis. We will demonstrate how SWATH® acquisition provides unbiased data collection on all detectable species in your sample, and gives you the ability to quantify each component with high-quality data.
Speakers: Dr. Li Zang is a Senior Scientist overseeing the protein characterization and mass spectrometry group in the Analytical Development department of Biogen (Cambridge, MA). She obtained her Ph.D. from Northeastern University, and after joining Biogen in 2005, developed her expertise in biopharmaceutical development especially detailed protein structure characterization using separation and mass spectrometry.
Dr. Chongfeng Xu has Ph.D. in Analytical Chemistry from Fudan University. Later he joined NYU Medical Center as postdoctoral researcher working on mass spectrometric analysis of proteins. He has been in Biogen since 2009. His expertise includes development of analytical methods, and characterization of protein therapeutics using separation methods and mass spectrometry. He has published 30+ peer reviewed papers in scientific journals.
Dr. Stephen Tate is a Senior Research Scientist with SCIEX and has spent the last 11 years working on research projects included assisting with the design of the TripleTOF® system platform as well as being a pioneer of data-independent SWATH® Acquisition.
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