EUPA 2018

On Demand Webinar

High Throughput Optimization of the PA 800 Plus CE-SDS Method for Your Challenging Therapeutics

By working with more therapeutic candidates of a wider variety of biopharmaceutical modalities you increase your chances of developing the next blockbuster drug. You also increase your chances of running into candidates that present unique analytical challenges. In this webinar Christian Milbrandt from J&J/Janssen presents a non-platform method development approach using a 96-well-plate and capillary electrophoresis–sodium dodecyl sulfate (CE-SDS) to provide high-throughput analysis of challenging biopharmaceutical drug candidates.

Approaches to non-platform method development include:

  • Initial evaluation of molecular weight (MW)
  • Secondary evaluation of molecule specific parameters for potential issues such as clipping, deamidation, free thiols or thioethers, just to name a few, including analysis of:
    • Light chain type (lambda vs. kappa)
    • Inter-chain disulfide bond(s)
    • Amino acid structure

Key Learning Objectives:

  • Development of non-platform methods for analysis of complex modalities
  • 96-well-plate format for high throughput CE-SDS
  • Flexible software and optimization tips for CE-SDS


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