Many labs are now using data independent acquisition (DIA) to perform large-scale quantitative LC–MS-MS proteomic experiments with solid reproducibility on thousands of proteins in complex matrices. As this technique increasingly proves to be a solid tool for biomarker research, larger sample sets are being analyzed, driving the need for further investigation of workflow improvements for throughput and robustness. SWATH® acquisition coupled with microflow chromatography provides an additional workflow option to researchers with higher throughput and increased robustness needs. When sufficient sample is available to move to the higher flow rate regime, very high reproducibility is achievable with faster run times, while still achieving reasonable depth of coverage.
In this presentation we will show:
Depth of coverage assessment of microflow LC–MS relative to current nanoflow strategies
Optimization of SWATH® acquisition settings for microflow chromatography
Key workflow recommendations for performing large-scale, high-throughput SWATH® acquisition studies
We hope you are able to join us for this exciting demonstration!