Join our lunch seminar on 15th of July at the Hotel Sofitel Budapest Chain Bridge, Budapest, Hungary.
This conference is a forum of exchange for the dissemination of the latest scientific information on bioactive lipids in health and disease as it relates to cancer, inflammation, and inflammation associated diseases such as cardiovascular, diabetes, psoriasis, and Alzheimer's, nephrology, arthritis and dermatology.
Informational talk: The Convergence of Differential Mobility Spectrometry and Mass Spectrometry: Next Generation Global and Targeted Lipid Analysis
The importance lipid mediators play in host immune inflammatory responses has translated to efforts to identify and quantify lipid molecular species as clinical biomarkers of human disease. Lipid biomarker discovery starts with global lipid profiling strategies using electrospray ionization mass spectrometry (ESI-MS/MS). The complex data arrays generated during sample analysis can then be processed by lipid identification software such as LipidViewTM and principal component analysis to generate candidate lipid biomarkers, which can then be validated by targeted quantitative analysis. Despite this seemingly straight-forward means to lipid biomarker discovery, the actual process is quite challenging due to the high number of lipid isobars and isomers that interfere with qualitative and quantitative analysis. Recently, Differential Mobility Spectrometry (DMS; SelexIONTM Technology) coupled to mass spectrometry has been shown to be very effective at resolving complex mixtures of lipid isomers without the need for extensive chromatography. This technology has been applied to broad-based quantitative lipidomics in the new lipidomics platform, the LipidyzerTM which detects and measures over 1200 lipids with absolute quantitation and represents the next generation of lipidomics technology.
SelexIONTM Technololgy is equally powerful at improving targeted lipid analysis. Eicosanoid analysis typically requires extensive method development to overcome background matrix interferences and isomer resolution, wherein carefully designed chromatographic separation strategies must be used in conjunction with sensitive and specific mass spectrometric analyses. DMS provides an additional degree of freedom in terms of isomer resolution, wherein lipids are resolved based on differences in their dipole moments. This combination of DMS with LC and ESI-MS/MS provides a powerful means to reduce background noise, potentially improve assay sensitivity and improve the specificity of the assay. In this presentation, the application of SelexIONTM Technology to global and targeted lipidomics will be discussed, and data will be shown demonstrating how it can be used to improve eicosanoid and lipid mediator analyses.
July 15, 2015
12:15 PM - 13:15 PM
Sofitel Budapest Chain Bridge Ballroom A & B, first floor Széchenyi István tér 2 1051 Budapest Hungary
Paul Baker PhD, Global Applications Lead Scientist Lipidomics, SCIEX