Multiclass Determination of Antibiotic Residues in Honey by LC-MS/MS
Honey has been always considered as a natural and healthy food product. However, in apiculture, antibiotics are used to fight bacterial infections. As result, honey samples can be contaminated with more than one class of antibiotics. Therefore, multi-class methods for analysis of antimicrobial residues are of paramount importance for the official control laboratories.
An efficient, simple and convenient method will be presented for the simultaneous analysis of veterinary drug residues from different classes (tetracyclines, quinolones, macrolides, lincosamides, sulfonamides, trimethoprim, dapsone, chloramphenicol and aminoglycosides).
The method was developed for the purpose of national monitoring program that includes imported and domestic honey samples. After acidic hydrolysis, honey samples were extracted by generic solid-phase extraction (SPE) and the extracts were subsequently evaporated and concentrated. The UHPLC-MS/MS analysis was done in a MRM mode with fast positive/negative polarity switching in a single chromatographic run. This allowed fast separation and high sample throughput. The analysis of aminoglycosides was done with HILIC separation and thus carried out in a second chromatographic run.
The method was validated according to European Commission Decision 2002/657/EC. The method performance characteristics indicate that it is suitable for routine use and regulatory purposes.
You will learn:
Have a better understanding of the analysis of antibiotics by LC-MS/MS in a routine laboratory
Learn about challenges in dealing with a difficult matrix like honey
See firsthand of the performance of the QTRAP 6500
Presenter: Radostina K. Manova
Ph.D - Research Scientist, The Netherlands Food and Consumer Product Safety Authority
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