Improving Peptide and Peptide-based Biopharmaceutical Analysis
Despite recent developments in liquid chromatography column and hardware technology analysis of larger intact peptides (like insulin and its derivatives for instance) by liquid chromatography mass spectrometry (LCMS) remains challenging. These larger peptides often exhibit properties which adversely affect their chromatography. In some instances they are polar eluting in the solvent front or are overly retained causing peak broadening or carryover problems.
In this webinar, we present a CESI-MS workflow to overcome challenges of stationary phase based separations. We will detect clinically relevant peptides in biological matrices at their physiological relevant levels. Subsequently we will study storage degradation products of intact insulin, both through charge state heterogeneity and cleavage product detection.
Key Learning Points
Methods to quickly obtain high-quality impurity profiling of intact peptide from minute amounts of sample
How CESI-MS is being used in peptide analysis
Complementarity of CESI-MS to analytical flow LC-MS
July 16, 2015 11:00 AM ET
Steve Lock Business Development Manager SCIEX Separations