Efficiency Gains with Sequence Variant Analysis by Mass Spectrometry
Mass spectrometry has become an important tool to characterize protein therapeutics. In order to develop a protein therapeutic it is critical to fully understand both the protein sample itself and the process leading to its generation. The clone selection process involves analyzing the produced protein product to confirm the amino acid sequence is correct and determine which leads to pursue.
The development of modern mass spectrometry instrumentation, such as the TripleTOF® 5600+, that provides both high resolution and accurate mass information over a wide dynamic range is able to generate data to assist in the characterization of biologics and post-translational modifications. Generating high-quality data is only part of the story as the need for processing tools to assist in turning the data into useful information is just as important. The database search program ProteinPilot™ can provide necessary efficiencies in data analysis when looking for amino acid sequence variants and post-translational modifications. In this webinar we will explore some examples where ProteinPilot™ was used to identify protein sequence variants present in cell line protein products even in the absence of a protein standard. ProteinPilot™ can also be used to identify amino acid sequence differences in antibodies that are a result of somatic mutations during antibody affinity maturation.
Key Learning Points
The importance of chromatographic reproducibility for comprehensive proteome quantitation
How using the Eksigent nanoLC 400 system helps achieve better results faster
The advantages of using chip-based nanoLC columns in longer term studies
January 22, 2014 11:00 AM EST / 8:00 AM PST
Jason Hogan Scientist II Bristol-Myers Squibb
St. John Skilton, Ph.D. Senior Global Marketing, Manager, Biologics SCIEX