Comprehensive Characterization of mAb in a Single Analysis
In this Webinar, we will present a comprehensive characterization of a representative monoclonal antibody trypsin-digest in a single analysis. Both small and large peptides (3-65 amino acids) are separated, identified, and quantified, contributing to 100% sequence coverage. Special emphasis is given to comprehensive, quantitative analysis of glycosylation microheterogeneity. Quantitative analysis of degradative hotspots such as asparagine deamidation, methionine oxidation, glutamic acid cyclization, and C-terminal lysine heterogeneity is achieved from the same run.
Key Learning Points
How to obtain comprehensive heterogeneity, stability, and purity information from a single-enzyme digest in a single run
How ultra-low flow rates reduce ion-suppression bias, increase sensitivity, and expand dynamic range
About orthogonal technology to status quo
September 30, 2014 11:00am - 12:00pm ET 8:00 - 9:00am PT