Agarose Gel Electrophoresis (AGE) has been the primary method used to assess the homogeneity of plasmid DNA, however, this approach has some major disadvantages. The AGE method is manual, only semi-quantitative and the assignment of bands to plasmid structures is difficult, as the electrophoretic mobility of plasmids of different shapes changes with the electrophoresis operating conditions A more powerful routine technology for the quantification of plasmid forms is Capillary Gel Electrophoresis (CGE). This automated approach offers high resolution, high sensitivity and high reproducibility to this analysis.
Separation of pUC19 (2.7 kbp) plasmid structures, using the P/ACETM capillary electrophoresis system (Schmidt et al, P/ACE™ Setter Newsletter, Vol. 4 Issue 2, October 2000).
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