This course is intended to provide users with a technology overview. The class covers basic workstation hardware including mass spectrometer components and vacuum systems. Software for instrument tuning, calibration, data acquisition, and analysis is reviewed in lectures and hands-on laboratory exercises. Training in high-throughput automation is by batch operation exercises. Sample digests are used for rapid training in protein identification (MS and MS/MS mode) with available database search tools including the Mascot Server and GPS Explorer™ Software.