Knowledge Base Article

Canned Calculation for ICH Guideline M10 – 3.2.2 Specificity Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in ICH guideline M10 – 3.2.2. The guidance requires that it is demonstrated that no significant response attributable to interfering components compared to the analyte or IS response in the blank and double blank samples, respectively, is observed. These calculations were created to provide a ready-made solution for determining which samples will need to be re-injected in pesticide reports.

Replacing the electrode in the SCIEX ion source that is compatible with the Eksigent microLC 200 LC system

A YouTube video demonstrates how to replace the electrode in the SCIEX ion source that is compatible with an Eksigent microLC 200 system connected to a SCIEX mass spectrometer and operated by the Analyst® software.

Is sensitivity lowered for MRM acquisition methods with polarity switching for a SCIEX 5500 system and other instruments?

If user adds polarity switching to a MRM acquisition method that includes both negative and positive experiments, it is possible in theory to have a different signal intensity (usually lower). But having the appropriate settling time in the MRM method will prevent this in most cases.

Troubleshooting Common LC Issues on SCIEX Triple Quad™ and QTRAP® Systems

Pressure issues, shifting retention times, and no peaks are common issues when running LC-MS methods. The guides linked tp this article provide step-by-step instructions to troubleshoot these common issues.

Valve Model Will Not Stop Equilibrating In SCIEX OS Software 2.1

When equilibrating any MS method on a SCIEX 7500 system, the "Valve Model" will never finish equilibrating even after the specified time when using SCIEX OS software 2.1.

What Happens During Q0 Dissociation when "Simple" or "Enhanced" Options Are Used in SCIEX OS Software?

Declustering is achieved with the SCIEX Triple Quad 7500 system using a voltage differential between the QJet® ion guide and the IQ0 lens or a voltage differential between IQ0 and Q0 (the quadrupole ion guide before Q1). This article explains how Q0 dissociation is achieved using simple or enhanced modes in the SCIEX OS software and how the D Jet™ ion guide is involved in enhanced-mode dissociation.

Location of the Current Instrument Data File for SCIEX Triple Quad™ or QTRAP® LC-MS/MS 7500 Systems

The current instrument data file (current.dat file) for Triple Quad™ or QTRAP® LC-MS/MS 7500 Systems is backed up to a SCIEX OS Software folder located at C:\ProgramData\SCIEX\MassSpec\InstrumentData\YYYYMMDDHHMMSS.

Can the Probes and Electrodes for the OptiFlow® Ion Source Be Used with the OptiFlow Pro Ion Source?

The probes and electrodes for the OptiFlow® Ion Source can be used with the OptiFlow Pro Ion Source.

Can the SCIEX QTRAP® or TripleQuad™ 7500 LC-MS/MS System Operate without E Lens™ Technology Active?

Yes, the SCIEX QTRAP® or TripleQuad™ 7500 LC-MS/MS Systems can operate without the E Lens™ Technology active. Each QTRAP and TripleQuad 7500 System comes with a blanking plug that can be inserted should there be a need to operate without the E Lens Technology.

Are the Ion Sources Interchangeable Between the QTRAP® and TripleQuad™ 6500+ and 7500 Systems?

The ion sources for the SCIEX QTRAP® and TripleQuad™ 6500+ and 7500 LC-MS/MS Systems are not interchangeable. The OptiFlow® Pro Ion Source cannot be used on the QTRAP or TripleQuad 6500+ Systems. Likewise, the IonDrive™ Turbo V, Turbo V™, and OptiFlow Ion Sources cannot be used with the QTRAP and TripleQuad 7500 Systems. 

Is Digital PicoView® Source Windows 10 compatible?

Digital PicoView® Source is a nano ion source from New Objectives that is Windows 10 compatible and can be used with many SCIEX mass spectrometers.

Setting Up MRM Transitions if Q1 Mass Is Identical to that of a Second Compound

To set up a MRM transitions where the Q1 mass for one compound is identical to a second compound in Analyst® software, change the Q1 mass of the second compound by 0.1 -0.05 Da. After making this adjustment, the software will accept the duplicate mass for Q1.

Why Doesn't the Recalculated Concentration in Excel Fit the Calculated Concentration in Analyst?

If I check the calculated results from Analyst for calculated concentration in Excel, the results do not match exactly. Why does this happen and how can I avoid this?

Explore Mode - Extract Ions - Overlay Multiple Panes Automatically

Where to find the settings for automatically overlay panes when extracting multiple Ions.

Lost sensitivity on your Triple Quad or QTRAP You may need to optimize your CEM detector parameter.

This article explains how to resolve the issue of low sensitivity on a Triple Quadrupole or QTRAP instrument due to an incorrect CEM value by manually optimizing the CEM detector parameter in the Analyst software

What Is the Function of the Collision Gas (CAD) on QTRAP® System Instruments?

The various functions of the CAD gas are described for the QTRAP® System instruments and the different scan types.

Turbo V™ Ion Source Heater Replacement

How to replace the ion source heaters in the Turbo V Ion Source