The Power of Precision
ZenoTOF 7600 system
SCIEX 7500 system
SCIEX Triple Quad 6500+ system
SCIEX 5500+ system
QTRAP 6500+ system
QTRAP 4500 system
View all mass
Echo MS system
2.0 High Throughput Multiplexing system
View all integrated
BioPhase 8800 system
8000 Plus system
800 Plus system
Advance your research with front-end
instruments designed to help you realize the full power of your mass
spectrometer. SCIEX has the broadest portfolio of ESI-MS front-ends
can facilitate various flow rates, sample requirements and
sensitivities. No one else offers the entire range
flow, microflow, nanoflow LC-MS and even ultra-low flow
ExionLC 2.0 series
View all front-end HPLC MS
Differential mobility spectrometry (DMS) and
ion mobility spectrometry are analytical techniques used to separate
ions based on their gas phase mobility. Multiple types of ion
devices exist, such as drift tubes, traveling wave, and high-field
asymmetric waveform devices. Learn how you can separate yourself
Differential Ion Mobility.
The latest ion sources from SCIEX enable
enhanced sensitivity and robustness with greater desolvation range
across all MS platforms, from Triple Quad to QTRAP and QTOF.
Turbo V ion
OptiFlow Turbo V ion
View all ion sources
vMethod applications are pre-configured and verified LC-MS/MS
that reduce the need for method development – significantly cutting
time, effort and money to deploy a new assay. Every vMethod provides
method conditions, recommended sample prep, LC and MS conditions,
details for applicable MS/MS library databases for key applications.
and personal care products (PPCPs)
and protein bioanalysis
Explore vMethod applications
Free software trials
feature request portal
Analyst TF software
Biologics Explorer software
Molecule Profiler software
High resolution and QTRAP
libraries can dramatically enhance the quality of your analysis,
you much improved confidence in your data. With a comprehensive
at your fingertips, you can easily create methods and process
and non-targeted screening data on your complex samples, faster and
easier than ever before.
All in one
HR-MS/MS library with NIST 2017
Explore the library
Boost the performance of your mass
spectrometer and improve sensitivity, productivity, and data
iChemistry Solutions are the world's only reagents and
that are custom designed with your success in mind.
RNA 9000 Purity & Integrity kit
kit for amino acid analysis of hydrolysates
kit for amino acid analysis of physiological fluids
protein digestion solution
Protein CE-SDS Purity Analysis kit
protein assay - human induction kit
CZE rapid charge variant analysis kit
BioPhase Fast Glycan Labeling and Analysis kit
standards kit for pesticide analysis
auEChERS extraction and dispersive kit
Ampliflex keto reagent
Cleavable ICAT reagent
View all consumables
QTOF – Quadrupole
Time of Flight
QTRAP® – Triple Quad Linear
SWATH® – Data Independent
SelexION® – Differential
MicroLC – Microflow
View All Technology
From substantiating new discoveries through
to end-to-end validated workflows for high-throughput biomarker
in the clinic, thousands of scientists worldwide depend upon mass
innovation from SCIEX to advance their work in the fields of
research, omics and diagnostics.
Clinical research (coming soon!)
Clinical diagnostics (coming soon!)
View All Clinical
Pesticides & herbicides
Soil and biota
View All Environmental
How do you protect your reputation and meet today’s global food
standards? Whether you are a commercial lab or a food manufacturer,
quality of your food testing data is vital to your business. SCIEX
solutions help you meet maximum residue limits (MRLs) with
data that you can genuinely count upon. With a portfolio of
applications, your lab can quickly and easily react to diverse
Authenticity & Profiling Analysis
& Food Contact Substance Analysis
Food and Beverage Testing
How do you ensure the integrity of your
results in an industry that is never constant? By accurately
even the smallest compound angles you can deliver evidence that
SCIEX forensic analysis solutions deliver fast, highly accurate
data across a multitude of compounds and biomarkers, from the known
the new and novel.
Forensic toxicology (coming soon!)
Homeland security (coming soon!)
Cannabis and hemp potency testing
Doping control (coming soon!)
View All Forensics
Metabolic flux analysis
and SNP Analysis
View All Life
Transform the capacity and capability of your biologics pipeline
complete end-to-end solutions that make your lab more productive,
more successful. With a longstanding track record in pharma
development and manufacturing, our unparalleled application
with best-in-class hardware, software and support all integrate to
revolutionize your lab.
Native Mode Analysis
Nucleic Acid and Plasmid Analysis
View All Pharma
Clinical diagnostics (coming soon!)
ClearCore MD software
Mass Spec Operators
View All Diagnostics
The SCIEX Now Learning Hub offers the most diverse and flexible learning options available, with best-inclass content that helps you to get the most out of your instrument and take your lab to the next level. Available personalized learning paths based on the latest memory science ensure better knowledge retention, and automated onboarding and enrollment means you’ll get up and running faster.
SCIEX Now Learning Hub offers the most diverse and
flexible learning options available, with best-in-class content that
helps you to get the most out of your instrument and take your lab
the next level. Available personalized learning paths based on the
latest memory science ensure better knowledge retention, and
onboarding and enrollment means you’ll get up and running faster.
SCIEX Learning Manager provides you with the infrastructure to assign, monitor and report on your staff's
competency through a single digital platform. Effectively manage the training process for new hires, ensure
continuous staff development and access information with a single log-in to your SCIEX account.
You can browse, filter, or search our
extensive list of training offerings. Choose from over 100
eLearnings or search for an instructor-led course near you. Once you
select the course you want to take, you will be directed to
Learning Hub for enrollment (login required).
SCIEX Now Online is the Everything, Anytime
destination for all your SCIEX support needs. You can keep track of
activities that matter most to you and manage your lab in the most
efficient way possible. Extensive self-help resources like our deep
Knowledge Base, enable you to solve many problems on your own. SCIEX
is available 24/7 and your new SCIEX instruments are automatically
to your profile when you purchase.
SCIEX Now Learning Hub
Manage My Instruments
SCIEX Now New Feature Request
Software New Feature Request
Online ordering solutions
Don't have an
account? Create One
Visit your SCIEX
No one understands your needs and can support
your systems better than we can. Our mission is to help you be
successful, whether it's to repair your instrument, assist with your
workflows, or help you maximize productivity in your lab. Whatever
challenge, global SCIEX Service and Support personnel are subject
experts who are focused on mass spec and capillary electrophoresis,
you'll be able to achieve your scientific goals quickly and
Plus Suite for Your New LC-MS
Instrument Service & Support
View All Support Tools
SCIEX Now Learning Hub is much more than online
courses. For the most comprehensive option, you can select Success
Programs: personalized, blended online and in-person courses. If
needs are met by a visit from one of our training experts, you can
choose multiple Onsite Training days to get your lab running. Visit
SCIEX training center in North America, Europe or Asia for intensive
classroom and laboratory training. And your online courses are
any time, from anywhere, right here.
Login to SCIEX Now Learning Hub
Success Programs at Your
Scientist Training at Your Site
Visit all Training
If you have CE, LC or mass spec questions,
then SCIEX has the answers. SCIEX support is the single destination
your system questions. We aim to fully assist you with virtually
application of our instruments, helping you to get the most out of
lab resources and assets.
View All Request Support
As a life science researcher, you need the
tools and support to help you create the scientific foundation in
pursuit of expanding the knowledge-base, whether it is understanding
fundamental biology, finding new biomarkers, discovering ways to
our quality of life, or other areas of research. We are committed to
same goals and put the very same dedication into our work to help
address your most significant research challenges.
View All Partnership
Regulatory compliance is as paramount for us
as it is for you. That’s why we have made it easy for you to freely
reference all relevant technical and product regulatory documents.
give you confidence that, with SCIEX, you will fully comply with
legislation, adhere to your laboratory protocol and meet industry
Declaration of Conformance
Safety Data Sheets
Certificates of Analysis
View All Regulatory Documents
SCIEX supplies extensive documentation to help you prepare for, use, and maintain your SCIEX hardware and software products, and we update this documentation regularly. On the Customer Documents page, you can search for and download the latest documents for your product.
Join the SCIEX community today to
interact with your peers, share and exchange ideas, develop
your knowledge, stay up-to-date with the latest products,
post insights and questions, comment on others and receive
support. This community is designed to help you, our
customers, move science forward and get the answers you
need. We’re committed to engaging with and listening to you,
to create the best customer experience possible and to
contribute to the success of your work.
Environmental / Industrial
Food and Beverage
Life Science Research
Now Feature Requests
View All Community
Our favorite papers
Meet our executives
In the news
You've got questions. We've got experts who
can help. Contact us to find out more, talk to a specialist, explore
solutions or get expert support.
Request a quote
SCIEX success network
Request hosted catalog
Global public relations
Bosnia and Herzegovina
United Arab Emirates
United Republic of Tanzania
AB Sciex is doing business as SCIEX. © 2010-2018 AB Sciex. The trademarks mentioned herein are the property
of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman
Coulter® is being used under license. Product(s) may not be available in all countries. For
information on availability, please contact your local representative. For
research use only. Not for use in diagnostic procedures.
The iTRAQ reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, both relative and absolute, while retaining important PTM information. There are two types of iTRAQ reagent kits, 4plex and 8plex. The iTRAQ reagents - 8plex kits are also available in more economical bulk packs.
Community Discussions »
Innovation in peptide-tagging chemistry
iTRAQ reagents are an isobaric, peptide-tagging technology that enable you to label all primary amines, regardless of peptide class. Because important information, such as post-translational modifications (PTMs), is retained, this tagging system allows you to extract more detailed information from your samples.
Figure 1: iTRAQ reagent - 8plex protein quantitation.
Higher-throughput protein biomarker discovery
Obtain protein identification and quantitation simultaneously for up to eight samples for higher throughput biomarker discovery. The straightforward iTRAQ reagent workflow has less rigorous chromatography demands than non-labeling approaches and provides high-confidence results. And contrary to label-free profiling approaches, iTRAQ reagents are completely compatible with fractionation strategies that allow you to "dig deeper" and evaluate lower-abundance proteins
Experimental flexibility with confident identifications
The ability to analyze up to eight different samples simultaneously gives you even more experimental-design flexibility. Multiplexing enables you to compare numerous sample states and provides the flexibility to run duplicates and triplicates. MS/MS fragmentation of peptides labeled with iTRAQ reagents - 8plex results in cleaner spectra with increased signal, giving higher confidence in peptide identifications. And the tagging and analysis of multiple peptides per protein further enhances protein identification.
Figure 2: iTRAQ reagents - 8plex protein identification.
Solutions for quantitative biology
Whether you are performing relative or absolute quantitation, iTRAQ reagents - 8plex, MS instrumentation, and application software provide an integrated solution with the ease, flexibility, and confidence in protein quantitation and identification you need for quantitative biology. These reagents are ideal for many applications including pull-downs, membrane protein studies, discovery and validation analysis for biomarker elucidation, and absolute quantitation of target proteins of interest. The reagents are compatible with most mass spectrometers but are optimized for SCIEX instruments.
Is iTRAQ available in bulk?
Yes. Bulk reagent is available in 25 and 50 unit kits of iTRAQ reagents only. You will need approximately one Buffer kit per 10 units.
Why is there a large difference in incubation times between 4-plex and 8-plex iTRAQ?
iTRAQ 8-plex is a different molecule than 4-plex, the reaction time is longer (120 minutes versus 60 minutes)
Are 114 -117 from 4-plex the same as 114 – 117 of 8-plex?
No, 4-plex and 8-plex are different reagents and should not be combined in an isobaric experiment
Why do I see a different charge state distribution of the target peptides after using iTRAQ?
Because of the different final pH, you may see a difference between the 2+ and 3+ charge states. A shift to more 2+ ions can usually be accomplished by adjusting the pH to 4.0 with 5mM ammonium acetate
Can I re-use the iTRAQ reagent after the vial has been opened?
No. Each vial of iTRAQ reagent is filled and capped under inert gas. The reagent is easily degraded by moisture leading to poor subsequent labeling efficiency. In the case of bulk reagent kits, there is a specific protocol to follow for aliquoting and storage.
My iTRAQ solution seems to be a different colour, is it OK?
Probably. The colour of the iTRAQ reagent can vary slightly depending on many factors ranging from trace metals to moisture. The reagent performance is typically unaffected.
How much protein can I label?
Each vial of 8-plex reagent contains 2 µmol of iTRAQ reagent. This is usually sufficient to label 20-100 µg of protein digest. For plasma and serum samples which contain many non-peptide primary amine compounds, the protocol may need to be adjusted. Important elements include buffering to pH 7.5 to 8.5, buffer concentration between 90 – 100 mM, iTRAQ reagent concentration at 40 mM (±5%), and total organic concentration greater than 65%
How long can I store sample after labelling?
we recommend you label only when you are going to analyze the samples immediately, please do not store your labelled samples for longer than two weeks.
The destination for all your support needs