For research use only. Not for use in diagnostic procedures.
Built for Maximum Sensitivity and Throughput of Precious Samples
You have limited sample volume available and need high-throughput processing. The M3 MicroLC System has been developed for the sensitivity, ease of use, and throughput needs of biopharmaceutical development.
Maintain high throughput while increasing sensitivity (up to 10x improved LLOQ)
Use less sample, allowing for multiple analyses
Save up to 90% of solvents
Space saving: stackable on top of SCIEX Triple Quad™ and QTRAP® Systems
Controlled by Analyst® Software, designed to support 21 CFR Part 11 compliance
SCIEX LC-MS Systems: Moving You Closer to Developing Your Next Successful Drug Candidate
The M3 MicroLC is a feature rich microflow LC-MS solution meant to simplify both small and large molecule quantitation and characterization. In addition to using a small sample size, this extremely sensitive, multi-purpose instrument will help you save on costs, space, and fully integrates with your SCIEX mass spectrometer of choice.
The M3 MicroLC is the perfect balance of sensitivity and robustness—more sensitive than traditional analytical flow LC and more flexible and more robust than nanoflow LC — giving you the potential to optimize your biopharma workflows, today and into the future.
Stackable on your SCIEX 6500+ to maximize floor space
Choose the columns you need to accomplish your goals
Pair with SCIEX Mass Spectrometers, analyst software and utilize BioBA kits
Utilize trap-and-elute workflow for increased robustness
Your existing SCIEX source is M3 MicroLC ready
Your Next Discovery is within Reach
The adoption of LC-MS system is becoming more desirable in today’s laboratories due to its high selectivity, accuracy, and precision for analyzing bioanalysis candidates. The M3 MicroLC offers a complete solution to optimize your biologic workflows, today and into the future.
Intact Mass Analysis
Determination of intact molecular weight is critical for successfully developing and producing protein therapeutics
Pair your M3 MicroLC with specially designed columns and industry-leading mass spectrometers for maximum sensitivity for your most challenging applications. Alternatively, turn to our NanoLC 400 Series if you need even more sensitivity.
BioBA Biologics Bioanalysis for Large Molecule
The BioBA Solution from SCIEX delivers everything you need to move to large molecule bioanalysis.
Sensitivity comparison of an Infliximab signature peptide. 20 uL of a 5 ng/mL Infliximab standard digest. View enlarged image
The M3 MicroLC offers unparalleled sensitivity for biotherapeutic development. Micro flow rates increase the sensitivity of your small sample volume analyses by up to 10x, as compared to analytical flow LC-MS. This makes the M3 MicroLC system the only choice when high sensitivity LC-MS/MS is required.
Engineered for robustness, the M3 MicroLC ensures accurate and reproducible results for peptide quantitation.
Proprietary Microfluidic Flow Control™ provides retention time reproducibility
Minimal dead volume flow paths for precise analyses
Autosampler dynamic load and wash (DLW) allows extremely low carryover
Trap-elute workflow minimizes sample contaminants in the system
Multiplexing for High Throughput
Routinely analyze hundreds of samples – day after day, year after year
Maintain high throughput with the trap-elute workflow. Sample workflows for loading 20 uL of sample. View enlarged image.
Designed for high throughput, the M3 MicroLC enables the use of trap-elute workflows. Sample is loaded onto at a high flow rate onto a trap column and then eluted for analysis at a micro flow rate. The result is up to 5x shorter run time than what can be achieved with direct inject micro flow.
High throughput with increased sensitivity
Regenerate the trap column during analysis to minimize run time
Complete Microflow UHPLC Solution
Everything needed for bioanalysis
SCIEX offers a complete solution for your bioanalytical needs. Designed for ease of use with the SCIEX Turbo V™ source, we offer electrodes optimized for micro flow LC.
SCIEX offers MicroLC Columns in a wide range of particle sizes (2.7 µm - 5 µm), pore sizes (90Å - 300Å) and surface chemistries, including C18, C8, C4, CN, Phenyl, AQ, C18-superficially porous particles and Silane (SIL). Created specifically for fast peptide quantitation, we offer columns packed with HALO Peptide ES-C18.
Extracted Ion Chromatogram of 24 MRM transitions of tryptic peptides in a digested human plasma sample on a 0.3 x 150 mm HALO Peptide ES-C18 column. Flow rate was 10 µL/min; gradient 5- 35 % ACN in 10 min.