Innovation in peptide-tagging chemistry
iTRAQ® Reagents are an isobaric, peptide-tagging technology that enable you to label all primary amines, regardless of peptide class. Because important information, such as post-translational modifications (PTMs), is retained, this tagging system allows you to extract more detailed information from your samples.
Figure 1: iTRAQ® Reagent - 8plex protein quantitation.
Obtain protein identification and quantitation simultaneously for up to eight samples for higher throughput biomarker discovery. The straightforward iTRAQ® Reagent workflow has less rigorous chromatography demands than non-labeling approaches and provides high-confidence results. And contrary to label-free profiling approaches, iTRAQ® Reagents are completely compatible with fractionation strategies that allow you to "dig deeper" and evaluate lower-abundance proteins
Experimental flexibility with confident identifications
The ability to analyze up to eight different samples simultaneously gives you even more experimental-design flexibility. Multiplexing enables you to compare numerous sample states and provides the flexibility to run duplicates and triplicates. MS/MS fragmentation of peptides labeled with iTRAQ® Reagents - 8plex results in cleaner spectra with increased signal, giving higher confidence in peptide identifications. And the tagging and analysis of multiple peptides per protein further enhances protein identification.
Figure 2: iTRAQ® Reagents - 8plex protein identification.
Solutions for quantitative biology
Whether you are performing relative or absolute quantitation, iTRAQ® Reagents - 8plex, MS instrumentation, and application software provide an integrated solution with the ease, flexibility, and confidence in protein quantitation and identification you need for quantitative biology. These reagents are ideal for many applications including pull-downs, membrane protein studies, discovery and validation analysis for biomarker elucidation, and absolute quantitation of target proteins of interest. The reagents are compatible with most mass spectrometers but are optimized for SCIEX instruments.