Several things can be done to reduce the contamination of LC/MS systems that require minimal time but increase uptime and robustness. Here, we present a list (in no particular ranking) of the top ten things to consider when trying to lower instrument contamination.
Select and open a compound in LibraryView™ Software. Then navigate to the MS/MS Spectra tab to view any MS/MS spectra for the compound. Select the ‘i’ button in spectrum panel, and a peak list with high precision m/z values will be displayed.
The current instrument data file (current.dat file) for Triple Quad™ or QTRAP® LC-MS/MS 7500 Systems is backed up to a SCIEX OS Software folder located at C:\ProgramData\SCIEX\MassSpec\InstrumentData\YYYYMMDDHHMMSS.
The BPV Flex™ Software 2.1 peptide score indicates the probability that the peaks in a given MS/MS spectrum will match those of the theoretical peptide fragments by random chance. Therefore, the score calculation is derived from observed MS/MS fragments and the corresponding intensities, as well as the number of theoretical MS/MS fragments.
The source on the X500R QTOF System can be used in atmospheric-pressure chemical ionization (APCI) mode, but a different probe with a twin electrode must be installed. In addition, a different APCI tuning solution must be used and is available for purchase from SCIEX.