Course Catalog

This training session describes the maintenance procedures for the API 2000™ and QTRAP® Systems TurboIonSpray® source.

This training session describes the optimization procedures for the API 2000™ and QTRAP® Systems TurboIonSpray® source. The topics include but are not limited to: probe alignment, manual and automated source optimization.

Duration: approximately 13 minutes

This training session describes the maintenance procedures for the API 3000™ System TurboIonSpray® source.

Duration: approximately 11 minutes

This training session describes the optimization procedures for the API 3000™ System TurboIonSpray® source. The topics include but are not limited to: probe alignment, manual and automated source optimization.

Duration: approximately 12 minutes

本在线学习课程主要介绍数据库搜索的基础功能。内容包含但不限于：数据库的储存位置、如何关联数据库、如何在数据库中添加和编辑化合物、如何展示数据库内容，以及如何在数据库中搜索未知物。

时长：约8 min

该自学培训课程介绍了在Analyst和Analyst MD软件的Explore模块下处理数据文件的基本功能。主题包括但不限于:打开数据文件、缩放谱图、设置背景区域、生成质谱图、生成提取离子色谱图、数据平滑、谱图标记、处理插件和片段解析工具。

时长:  约 15 min

This training courses focuses on method creation and data acquisition using Analyst and Analyst MD software. It covers the following topics:
- Creation of basic and advanced acquisition methods such as scheduled-ionization, Scheduled MRM (sMRM), multiple periods and IDA experiments
- Creation of an acquisition batch
- Submission of the batch to the queue
- Starting data acquisition

Duration: 15 mins
Learner Level: Intermediate
Certificate Type: Completion
PACE Accreditation: None

This self-paced training session focuses on the basics of using Analyst® and Analyst MD software for compound and source/gas optimization for triple quadrupole and QTRAP® instrument systems. Manual compound and source/gas optimization will be shown. Additionally, automated compound optimization by Infusion and automated source/gas optimization by Flow Injection Analysis will be covered using the Compound Optimization function.

Duration: approximately 25 minutes

This course introduces basic functions for processing data files in the Explore Mode of Analyst and Analyst MD software. Topics include but are not limited to: opening data files, zooming graphs, setting background regions, generating spectra, generating extracted ion chromatograms, data smoothing, graph labeling, processing scripts, and the fragment interpretation tool.

Duration: approximately 15 minutes
Learner Level: Intermediate
Certificate Type: Completion
PACE Accreditation: None

This self-paced training session covers the basics of the Library Search function. Topics include but not limited to: where library databases are stored, how to connect to a library, how to add and edit compounds to a library, how to show the contents of a library, and how to search unknowns against a library.

Duration: approximately 8 minutes

This self paced presentation will take Analyst® Software 1.5 users through the quantitative work flow of MRM data generated on SCIEX Triple Quad and QTRAP® System instruments. Users will learn how to choose the integration algorithms, use the Quantitation Wizard to integrate LC-MS/MS data to perform linear regression and generate reports through the Analyst® Software Reporter tool.

Duration: approximately 20 minutes

This self paced training session focuses on the basics of using Analyst® and Analyst MD software for Resolution and Calibration for triple quadrupole and QTRAP® instrument systems. Topics included are:
Resolution Optimization
Reviewing Resolution Peak Spectra
Mass Calibration from Spectrum
Mass Calibration Report and the Mass Calibration Result.

Duration: approximately 35 minutes

Сессия для самостоятельного изучения фокусируется на основах использования программного обеспечения Analyst® и Analyst MD для оптимизации соединений и параметров источника ионизации/газов для тройных квадруполей и систем QTRAP®. Включает в себя демонстрацию ручной оптимизации соединений и параметров источника ионизии/газов. Также во время сессии будет рассмотрена автоматическая оптимизация соединений с помощью метода инфузии и метода проточно-инжекционного анализа (ПИА).

Глава: 1
Продолжительность: 25 мин 

本トレーニングコースはAnalyst®およびAnalyst MDソフトウェアで制御する四重極およびQTRAP®装置における基本的な化合物とイオンソース/ガスの最適化に焦点を当てた内容で、受講者のペースで進めていただけます。インフュージョンを用いて手動で行うこれらのパラメータの最適化について説明されており、加えてFIAモードで行う化合物パラメータ自動最適化のプロセスもカバーされています。

時間：およそ25分

This self-paced training session introduces basic operation of Analyst® and Analyst MD software for the new user. Topics include but not limited to: user interface, project folders and their structure, system status icons, five modes of the software, how to find help files, hardware profiles, audit trail, and basic computer maintenance. Learners interested in obtaining a PACE certificate and PACE accreditation for taking this course (equal to 0.5 PACE credit) must successfully complete the learning module and a brief online evaluation survey.

Duration: approximately 24 minutes

このコースは、セルフペース型のトレーニングプログラムです。トリプル四重極およびQTRAP^®装置の分解能と質量精度の調整を行うための基本的なAnalyst^®およびAnalyst MDソフトウェア操作に焦点を当てご説明しています。
含まれるトピックは次のとおりです。：
必要な試薬
手動による分解能と質量精度の確認
手動による分解能補正と質量精度補正
Instrument Optimization機能を用いた自動キャリブレーション
自動キャリブレーションの結果とそのレポートについて

所要時間：約35分

このコースは、セルフペース型のトレーニングプログラムです。操作にまだ慣れておられないユーザー様向けに、Analyst®およびAnalyst MDソフトウェアの基本的な操作方法をご紹介しています。トピックには以下の内容を含みますが、これらだけに限りません。：ソフトウェアインターフェースの概要、Projectフォルダとその構造、System Status（システムステータス）アイコン、ソフトウェアの5つのモード、Helpの位置、Hardware Profile（ハードウェアプロファイル）の設定、セキュリティー設定やAudit Trail（監査証跡）機能、基本的なコンピュータメンテナンス。このコースを受講してPACEの証明書・修了証とPACE認定（0.5 PACEクレジットに相当）を取得したい方は、モジュールの学習を終え、習得度を確認するクイズに合格してください。

このトレーニングコースでは、AnalystとAnalyst MDソフトウェアを利用したAcquisition Methodの作成とデータ取得に焦点を当てご紹介します。以下のトピックを含みます。：
- 基本的なAcquisition Methodの作成方法、Scheduled -ionization機能、Scheduled MRM(sMRM)、複数のPeriodやIDA Experimentを含む応用的なAcquisition Methodの作成方法
- Acquisition Batchの作成方法
- Batch のQueue（キュー）へのSubmit方法
- データ取得の開始方法

所要時間: 15分
学習レベル:中級
コース修了認定証： 有り
P.A.C.E. 認定対象: 対象外

このコースでは、Analyst および Analyst MD ソフトウェアの Explore モードでデータファイルを処理するための基本的な機能をご紹介します。このコースでは、データファイルの開き方、グラフを拡大する方法、バックグラウンドの減算、スペクトルの表示方法、抽出イオンクロマトグラムの表示方法、データのスムージング方法、グラフのラベリング、便利なスクリプト、Fragment Interpretation Toolなどの基本的な機能をご紹介します。

チャプター数：1
所要時間：約15分
学習レベル:中級
コース修了認定証： 有り
P.A.C.E. 認定対象: 対象外

本自控速培训的主要内容为使用Analyst®软件为三重四极杆和线性离子阱系统进行验证和校准。包括根据指标验证和校准单位分辨率、质量数偏移、峰值强度，也介绍了如何检查校正结果。

学习时长：约35分钟。

이 자가 주도형 교육 세션에서는 새 사용자를 위한 Analyst® 소프트웨어 기본 작동 방법을 설명합니다. 주제에는 사용자 인터페이스, 프로젝트 폴더 및 구조, 시스템 상태 아이콘, 소프트웨어의 5가지 모드, 도움말 파일을 찾는 방법, 하드웨어 프로필, 감사 추적 및 기본 컴퓨터 유지관리 등이 포함되어 있으나 이에 국한되지 않습니다. 이 강좌 이수에 대한 PACE 인증서와 PACE 승인 획득(PACE 크레딧 한 개(1)와 동일)에 관심 있는 학습자는 학습 모듈과 간단한 온라인 평가 조사를 성공적으로 완료해야 합니다.

기간: 약 24분

이 교육 강좌에서는 Analyst 및 Analyst MD 소프트웨어를 사용한 분석법 만들기와 데이터 수집을 집중적으로 설명합니다. 다음 주제로 구성되어 있습니다.
- 예약 이온화, Scheduled MRM (sMRM), 여러 기간 및 IDA 실험과 같은 기본 및 고급 수집 방법 만들기
- 수집 배치 만들기
- 배치를 대기열에 제출
- 데이터 수집 시작

기간: 15분
학습자 수준: 기초
인증 유형: 완료
PACE 승인: 없음

本自助学习课程是针对新用户的Analyst软件的基础培训。课程内容包括以下内容的详细介绍：

• Analyst用户界面概览 - 项目文件夹及其结构、系统状态图标
• Analyst软件模块功能介绍 - 硬件配置、调谐和校准、建立采集方法和批次、Explore、定量
• 计算机维护的注意事项

获得这门课程的PACE证书和PACE认证之前（等于1个PACE学分），学员需要学完本课程和完成一个简短的在线调查。

时长：约24 min

本自助学习课程主要讲解了在四极杆和线性离子阱仪器上使用Analyst软件进行source/gas优化的过程。手动化合物和source/gas优化都将介绍到。另外，课程还概况了使用针泵和流动注射分析的自动优化功能。

时长：约25min

本自助学习课程主要讲解如何新建基础的采集方法，包括新建采集批次、向序列中提交批次和运行采集等步骤。

学习时长：15分钟左右

This self-paced, online training session will provide Triple Quadrupole and QTRAP® System users with some practical suggestions to improve the sensitivity of their experiments.

Course Level: Basic

Duration: approximately 20 minutes

Course ID: c35f1f35-fb02-4b4d-aeaa-e223fbb11c94

This eLearning course will cover the basic operation and maintenance for the CESI 8000 Plus system. 

After completing this course, you should be able to:
- Understand the different modes of operation for CESI-MS
- Understand the appropriate parameters and instrument settings for connecting the CESI 8000 Plus system to an MS System
- Be familiar with different capillary types
- Perform basic maintenance on your system

Chapters: 1
Duration: 30 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 2bebc817-14bb-4121-bce8-740dd4cc2de9

This course will show you how to process data for a structured Multiple Attribute Methodology (MAM) workflow using BioPharmaView ^TM Software 3.0.

This course covers the following topics:
- Overview of BioPharmaView Software 3.0
- What is MAM?
- Benefits of using a MAM approach
- Step by Step Data Processing for MAM Workflow using BioPharmaView Software:

Duration: 15 mins
Learner Level: Experienced
Certificate Type: Completion
PACE Accreditation: None
Course ID: 5aa80371-8999-4bc0-9820-b24354f577cb

In this course we will walk you through the intact protein molecular weight workflow, peptide mapping and quantitation, MSMS of all workflow for host cell protein detection, and the utilization of CESI as an additional separation strategy, all with AB SCIEX tripleTOF system.

This video will demonstrate how PTMs can be characterized and monitored using a dedicated multi attribute methodology solution in SCIEX OS. 

Chapters: 1
Duration: 15 mins
Learner Level: Basic
Certificate Type: None
PACE Accreditation: None
Course ID: 09a4d48d-776e-4652-a207-e68192a942c1

This course is an overview of charge heterogeneity based analysis using cIEF and CZE. It is a two part course, and covers the theory of the two assays, sample analysis using the methods, kits and PA 800 Plus system, and finally how to troubleshoot common issues. You must complete Part A before continuing onto Part B.

After taking this course, you should be able to:
- Understand the importance of charge heterogeneity characterization
- Describe the relationship between pH and protein charge
- Perform sample analysis using High Performance Capillary Isoelectric Focusing (cIEF)
- Perform sample analysis using Capillary Zone Electrophoresis
- Troubleshoot common issues

Chapters: 2
Duration: 30-40 mins for each chapter
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 31d4bcb4-53dd-4c36-993c-2af9a7385ef9

This eLearning course will cover several examples of how to create flagging rules and custom columns in SCIEX OS.

After completing this course, you should be able to:

• Create custom columns
• Create flagging rules using default and custom columns
• Perform data review using flagging rules and custom columns
• Import and export flagging rules into a method.
  

Chapters: 1
Duration: 20 mins
Learner Level: Intermediate to Advanced
Certificate Type: Completion
PACE Accreditation: None

This training module presents the new features and work flow of DiscoveryQuant™ Software 2.1 Analyze. The use of optimized parameters from the DiscoveryQuant™ Software 2.1 Database is described to build Multiple Reaction Monitoring (MRM) acquisition methods. The procedures for DiscoveryQuant™ Software 2.1 Analyze sample batch template creation and sample analysis submission are presented. Data integration and final report generation are also covered.

Duration: approximately 10 minutes

This training module presents the new features and work flow of DiscoveryQuant™ Software 2.1 Optimize. Initial hardware and starter method setup are reviewed. Automatic compound optimization through Flow Injection Analysis (FIA) is described including the use of the new QuickTune and FineTune options. Compound review using the DiscoveryQuant™ Software 2.1 Database is also covered.

Duration: approximately 19 minutes

本在线学习系列旨在介绍如何使用Echo MS®系统进行基本的定量工作流程。课程由四个单元组成:实验室安全须知、实验准备、数据采集和数据分析。

实验室安全须知和实验准备包括适当的实验室安全程序、如何准备系统、流动相和样本。

数据采集包括创建MS和AE方法以及如何采集数据。

数据分析主要演示如何创建处理方法、处理和查看数据、生成报告和执行自动触发的数据处理。

如果需要，在页面上的“资源”标签中可以查看用作数据处理的示例数据。

单元数：4
学习时长：每单元5-20min
课程级别：初级
证书类型：完成
PACE 认证：NO

本在线课程是对Echo^® MS系统的概览介绍。课程将讲授以下内容：

• Echo MS 系统的部件和原理
• 如何通过 SCIEX OS 配置Echo MS 系统
• 建立方法
• 使用直接设备控制( Direct Device Control )
• 执行维护工作

单元数：1
学习时长：15min
证书类别：Completion
PACE 认证：None

This eLearning course will walk you through the maintenance and troubleshooting of Eksigent expert™ microLC 200 System. You have the option to advance to any specific topic of interest.

The Eksigent NanoLC™ Course is designed to provide the new user a primer on how to use the NanoLC in their experimental workflows and how to address common pitfalls that can arise when using the instrument, especially during the familiarization stages of its use. It is assumed the user has a working knowledge of mass spectrometry and high flow LC. The course is intended to help the new user set up the NanoLC instrument, design methods, run samples using the LC and troubleshoot common problems.

Duration: approximately 12 minutes

This training module covers how to clean the front end components for SCIEX triple quadrupole and QTRAP mass spectrometer systems. 
The following topics are covered during the course:
 - Overview of the front end components
 - Cleaning tips 
 - System cleaning procedures for curtain and orifice plates, skimmer and ion guides.

Click on the "Resources" button on the left of the page for additional resources on "How to mimic a Divert Valve" and "Source/Gas Parameters and Settings".

Duration: 20 minutes
Learner Level: Intermediate
Certificate Type: Completion
PACE Accreditation: No

This course will show you how to clean the front end components of your TripleTOF® System. It will demonstrate the following procedures:
Routine cleaning of the curtain and orifice plate without venting the system
Full cleaning of the QJet® ion guide, curtain and orifice plate by venting the system

Chapters:1
Duration: 15 mins 
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 2f2bfb09-6ac3-401b-84c0-faffefbd1930

This course covers the theory of the Scheduled MRM^TM algorithm, and how to create methods using the algorithm. It also covers enhancements to the algorithm in Analyst® software version 1.7.

After taking this course, you should be able to:
- Understand how the Scheduled MRM algorithm works
- Create a method using basic and advanced parameters
- Describe some rules to follow when creating a method
- Optimize your method using the sMRM Calculator script

Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: e9a6840d-1741-4921-b450-b7268083c63b

Buổi tự đào tạo này sẽ cung cấp những kiến thức cơ bản về khối phổ cho người mới dùng bao gồm việc sử dụng phổ khối, tạo ion, bộ phận phân tích khối và đầu dò. Những người học quan tâm đến việc có được chứng chỉ PACE và chứng nhận PACE để tham gia khóa học này (tương đương với 0,5 chứng chỉ PACE) phải hoàn thành mô-đun học tập và khảo sát đánh giá trực tuyến tóm tắt.

Chương: 1
Thời lượng: 33 minutes
Trình độ học viên: Basic
Loại chứng chỉ: Hoàn thành
PACE Công nhận: Đúng

Khóa học giới thiệu chủ đề dựa trên LC-MS để xác nhận sàng lọc hợp chất sử dụng công nghệ hệ thống QTRAP của hang AB SCIEX. Sau khi hoàn thành khóa học, người học sẽ có thể tạo ra một phương pháp chạy mẫu, tạo một lô chạy mẫu, xử lý dữ liệu và tạo một báo cáo.

Khóa học bao gồm các chủ đề sau:
- Giới thiệu về quy trình sàng lọc hợp chất
- Tổng quan về hệ thống LC-MS / MS
- Tổng quan về  quy trình làm việc  của thu nhận thông tin phụ thuộc (Information Dependent Acquisition / IDA) 
- Cách xây dựng phương pháp MRM-IDA theo cách thủ công và sử dụng Trình hướng dẫn IDA
- Cách gửi một lô, xử lý dữ liệu và tạo báo cáo sàng lọc tổng hợp

Chương: 1
Thời lượng: 30 minutes
Trình độ học viên: Basic
Loại chứng chỉ: Hoàn thành
PACE Công nhận: Không

本培训主要讲解使用高效液相色谱(HPLC)系统进行质谱分析应用中的流动相准备和液相泵设置相关的基础知识。简要概述了高效液相色谱系统的组成，包括清洗LC泵的常规要点。特别强调了试剂和测量设备的正确使用。在本培训的最后附有一个简短的测验。

持续时间: 约10分钟

The course introduces the high-resolution MRM workflow using the SCIEX X500R QTOF system with SCIEX OS. This is an advanced workflow course, and it is recommended that you complete the LC-MS/MS Operation Series for the X500R QTOF system eLearning course before taking this course. Upon completion of the course, learners will be able to build an optimized MS method, submit a batch and process data.

The course covers the following topics:
- Guided MRM HR compound optimization
- TOF MSMS method editor
- Build a batch and run scheduled MRM HR
- Process data using the quantitation, and quantitation and targeted identification workflows

Chapters: 1
Duration: 25 minutes
Learner Level: Advanced
Certificate Type: Completion
PACE Accreditation: No

Khóa học này được thiết kế để giới thiệu cho người dùng một số bảo trì và khắc phục sự cố phổ biến thông thường có thể xảy ra trên các hệ thống khối phổ ba tứ cực và QTRAP. Nó bao gồm các chủ đề sau:
- Làm quen với phân tích dữ liệu hệ thống MS, nguồn ion hóa và đầu dò ESI và APCI
- Mười vấn đề phổ biến nhất đã được báo cáo cho nhóm Dịch vụ và Hỗ trợ Kỹ thuật của chúng tôi
- Chi tiết về một số sự cố và cách khắc phục sự cố phổ biến nhất liên quan đến tín hiệu, máy tính và lỗi liên quan đến kết nối và cách khắc phục
- Bảo trì vượt qua các nhiệm vụ bảo trì được đề xuất và cách thực hành tốt nhất.

Chương: 1
Thời lượng: 30 minutes
Trình độ học viên: Basic
Loại chứng chỉ: Hoàn thành
PACE Công nhận: Không

The purpose of this presentation is to introduce novice users of SCIEX QTRAP® instruments to the subject of linear ion trap method creation. It is intended to introduce topics which the user might need to be aware of during method development.

Duration: approximately 34 minutes

This course is an overview of quantitative protein purity analysis using SDS-Gel Capillary Electrophoresis. It covers the theory of the application, separation, and sample analysis using the CE-SDS MW Kit and IgG Purity and Heterogeneity Assay Kit, and finally interpretation of the results. 

After taking this course, you should be able to:
- Understand the theory of the application and the separation
- Perform sample analysis using the IgG Purity and Heterogeneity Assay Kit
- Interpret results obtained using the PA 800 Plus System

Duration: 30 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 8fe198db-3d97-4291-a669-203c784c4f5a

Drug discovery and development is an extremely complex and lengthy process. Part of this investigation involves testing new chemical entities to determine how quickly and extensively they are metabolized. Metabolites discovered through evaluation of a new chemical entity are characterized for activity and safety. This process is commonly referred to as metabolite identification or Met ID. AB SCIEX LC MS/MS solutions provide unique features that deliver the highest quality and most accurate results. Our Met ID software solutions improve efficiency from sample analysis all the way through to generating comprehensive reports.

The focus of this "Insight from the Experts" series is meeting the inherent challenges of accurate Peptide Quantitation in Bioanalysis. The increasing need for quantitative methods for the detection of proteins and peptides in complex biological samples can be attributed to the recent interest in the discovery and development of biotherapuetics . However, the quantitation of these larger molecules can be challenging. To meet these challenges AB Sciex offers innovative tools for Peptide Quantitation and complete workflow solutions that give you the confidence you need to meet even the most difficult method challenges.

The focus of this series is on Novel Approaches in Lipidomics Research presented by Dr Paul Baker. Dr Baker is a recognized expert in lipidomics who has helped shape the technologies offered by AB SCIEX.

The focus of this edition of Insights from the Experts is on novel approaches in metabolomics research. The term "metabolomics" also referred to as metabonomics or metabolic profiling covers a broad range of applications and studies in both plants and animals. Measuring changes and determining respective levels of concentrations of endogenous metabolites is most attractive, as it lends insight into the functional end-point of the physiology and interrogation of the metabolome at the phenotypic level. At AB SCIEX we offer a total solution for efficient metabolomic workflows, including state-of-the-art TripleTOF® and QTRAP® mass spectrometry. Our data processing software tools; MasterView™ and MarkerView™ software enable quantitative and qualitative data interogation.

LC/MS based proteomics has had a profound impact on the way we study biology. Whether you are studying signal transduction in plants or researching biomarkers for human diseases, mass spectrometry provides a powerful tool for discovery, characterization and quantitation of proteins. While protein ID is sufficient for locating the key players in biological pathways, achieving a deeper understanding requires researchers to investigate differential regulation of proteins. This need has resulted in a growing emphasis on quantitative workflows. From in depth global discovery to comprehensive targeted quantitation and validation, AB SCIEX provides a complete hardware and software solution to solve the most complex challenges faced by proteomic researchers today.

The focus of this series is on workflow solutions for biologics analysis presented by Dr Jiawei Chen. Dr Chen is an experienced Biologics application scientist supporting customers everyday to improve their mass spec workflows.

In this course, we introduce the SCIEX X500B QTOF System and BioPharmaView^TM software, and demonstrate the workflow for Intact Biologics analysis.

After taking this course, you should be able to:
- Understand the basics of Intact Biologics analysis
- Be familiar with the hardware and software features
- Create LC and MS methods to acquire data in SCIEX OS
- Submit a batch
- Process Intact Biologics data using BioPharmaView software

Chapters: 1
Duration: 20 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 4d4bac18-82e6-4ecb-9c9a-6c992f5b7468

Este curso está diseñado para ayudar a los usuarios que no están familiarizados con la Cromatografía Líquida a entender los fundamentos de la HPLC. El curso cubre los siguientes temas:
- Componentes básicos del sistema HPLC y sus funciones
- Teoría básica de LC para diferentes fases estacionarias y móviles
- Modos de separación comunes y sus aplicaciones
- Resolución en la cromatografía y cómo se puede alterar

Los alumnos interesados en obtener un certificado PACE y una acreditación PACE por realizar este curso (equivalente a un (1) crédito PACE) deben completar con éxito el módulo de aprendizaje y una breve encuesta de evaluación online.

Duración: aproximadamente 35 minutos
Nivel del alumno: Básico
Tipo de certificado: Finalización
Acreditación PACE: Sí

Esta sesión de capacitación proporcionará una introducción a la teoría de triple cuadrupolo, trampa de iones lineal, escaneos y operaciones en espectrómetros de masas SCIEX. Los participantes que estén interesadas en obtener un certificado PACE y una acreditación PACE (equivalente a un (1) crédito PACE) deberán completar con éxito el módulo de aprendizaje y una breve encuesta de evaluación en línea.

Duración: 40 minutos
Nivel de aprendizaje: básico
Tipo de certificado: Finalización
Acreditación PACE: Si

After taking this training course, the user should be able to:

Understand the SWATH™ workflow;

Generate ion libraries from discovery data;

Utilize the SWATH™ 2.0 MicroApp for comprehensive XIC generation;

Utilize the SWATH™ 2.0 MicroApp for data export for further statistical analysis.

Course ID: e7575c03-b046-4183-a530-2729c76ba399

This course will introduce you to the Analyst® TF Software and give you an overview of the software and its functionality.

After taking this course, you should be able to:
- Create a hardware profile
- Perform Instrument Optimization
- Understand MS/MS^ALL with SWATH^TM Acquisition, IDA and MRM ^HR scan types
- Create methods using the Method Wizard
- Create TOF MS and IDA methods
- Create and submit batches
- Explore your data

Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 35c14e9e-de0a-435b-b214-10c433b39d9a

Following the completion of this course, you will be able to explain how tags are used in Analyst Software Reporter 3.2, add and modify Text Tags to templates, add and modify Picture Tags to templates, explain how For Each Loops are used, and modify IF tags to flag results.

Duration: approximately 18 minutes

After taking this training course, the user should be able to: Identify the major structural components of antibodies as well as their endogenous function; Understand the basic interaction between antibodies and their epitopes on antigens; Identify antibody isotypes, IgG subclasses, and identify the difference between monoclonal and polyclonal antibodies; Understand the basis for mAb therapeutics and different types of fusion and chimeric proteins; Understand the different types of anti-drug antibody responses to therapeutic mAbs; Identify components of an antibody drug conjugate and various conjugation techniques and their effect on drug to antibody ratios; Explain potential differences in data acquired from ligand binding assays and LC/MS assays

This self-paced, online training will demonstrate the workflow of ChemoView™ 2.0 Software data processing of MS/MS data acquired from ABSCIEX API and QTRAP® System instruments. Users will learn how to define analyte types and sample types; define Tests and Analytes; define processing methods and review reports.

Duration: approximately 17 minutes

This course is an introduction to the ClearCore^TM MD Software, and is comprised of four modules: Overview and Optimization, Method Development, Data Acquisition and Quantitation. The ClearCore MD Software is one component of the Topaz System. This course is designed to teach a routine user and method developer how to use the ClearCore MD software and Topaz System for a general use quantitative LC-MS workflow. 

Upon completion of the series, you will be able to achieve the following tasks:
- Configure and activate devices
- Create an LC method for the Topaz LC
- Use Compound  Optimization to generate an MRM method for the Topaz Mass Spectrometer
- Optimize source and gas parameters
- Create a batch and submit samples for the Topaz System
- Quantitation using the Analytics workspace

Chapters: Four (4)
Duration: 20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 32d20ca1-694c-4b3d-bac2-e0bf7efdb0a1

This elearning course will cover:

1. How to use the Cliquid® Software to acquire and process data

2. How to create new tests or modify existing tests as an administrator

3. How to install purchased iMethods™ as an administrator

4. And how to process screening data and create a report

This course is designed to help users who are unfamiliar with Liquid Chromatography to understand the basics of HPLC. The course covers the following topics;
- Basic HPLC system components and their functions
- Basic LC theory for different stationary and mobile phases
- Common separation modes and their applications
- Resolution in chromatography and how it can be altered

Learners interested in obtaining a PACE certificate and PACE accreditation for taking this course (equal to one (1) PACE credit) must successfully complete the learning module and a brief online evaluation survey.

Duration: approximately 35 minutes
Learner Level: Basic
Certificate Type: Completion
PACE accreditation: Yes

The course introduces the subject of LC-MS based confirmation compound screening utilizing SCIEX QTRAP® Systems Technology. Upon completion of the course, learners will be able to create an acquisition method, submit a batch, process data, and create a report.

The course covers the following topics:
- Introduction to the Compound Screening process
- Overview of an LC-MS/MS system
- Overview of the Information Dependent Acquisition (IDA) workflow
- How to build an MRM-IDA acquisition method manually and using the IDA Wizard
- How to submit a batch, process data and creating a compound screening report

Chapters: 1
Duration: 25-30 minutes
Learner Level: Intermediate
Certificate Type: Completion
PACE Accreditation: No

The course introduces the subject of LC-MS based confirmation compound screening utilizing SCIEX QTRAP® Systems Technology. Upon completion of the course, learners will be able to create an acquisition method, submit a batch, process data, and create a report.

The course covers the following topics: 
- Introduction to the Compound Screening process
- Overview of an LC-MS/MS system
- Overview of the Information Dependent Acquisition (IDA) workflow 
- How to build an MRM-IDA acquisition method manually and using the IDA Wizard
- How to submit a batch, process data and creating a compound screening report

Chapters: 1 
Duration: 25-30 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: No

This course covers the basics of how to use the X500R and X500B QTOF Systems with the SCIEX OS software, and is comprised of four modules: Safety and Lab Requirements, Preparation, Optimization, and Data Analysis. Upon completion of the series, participants will be informed of appropriate laboratory safety protocol, as well as how to prepare the system, mobile phases, and samples. They will also be able to tune and calibrate the X-Series QTOF system, build methods and create/submit batches. The final section will cover the review of TOF MS and IDA data.

Chapters: Four (4)
Duration: 20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No

Course ID: 77a2fc7e-142e-43da-90da-96bb6b69aace

This eLearning

series is designed to teach a user how to use the SCIEX IVD triple quadrupole and QTRAP® systems for a basic quantitative workflow. It consists of four modules: Safety and Lab Requirements, Preparation, Optimization and Data Acquisition, and Data Processing.  
Safety and Lab Requirements, and Preparation cover appropriate laboratory safety procedures, how to prepare the system, mobile phases, and samples.  
Optimization and Data Acquisition covers compound and source optimization to create an acquisition method, and how to acquire data.  
Data Processing details how to create a processing method, process and review data, and generate a report using MultiQuant^TM MD software.

If required, you can find example data to use as practice for data processing in the Resources section on the left side.  

Chapters: Four (4)
Duration: 20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No

Course ID: 52743b78-5d0c-4e22-b0d0-9c77b4b2cc51

This eLearning series is designed to teach a user how to use the SCIEX triple quadrupole and QTRAP® systems (RUO systems only) for a basic quantitative workflow. It consists of four modules: Safety and Lab Requirements, Mobile Phase and Standards Preparation, Optimization and Data Acquisition, and Data Analysis. 
Safety and Lab Requirements, and Mobile Phase and Standards Preparation cover appropriate laboratory safety procedures, how to prepare the system, mobile phases, and samples. 
Optimization and Data Acquisition covers compound and source optimization to create an acquisition method, and how to acquire data. 
Data Analysis shows you how to create a processing method, process and review data, and generate a report using MultiQuant ^TM or SCIEX OS-MQ software.

If required, you can find example data to use as practice for data processing in the Resources section on the left side. 

Chapters: Four (4)
Duration: 20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No

This course covers the basics of how to use the SCIEX TripleTOF®Systems, and is comprised of four modules: Safety and Lab Requirements, Preparation, Optimization, and Data Analysis.

Safety and Lab Requirements, and Preparation cover appropriate laboratory safety procedures, how to prepare the system, mobile phases, and samples.  
Optimization and Data Acquisition covers how to tune and calibrate the SCIEX TripleTOF system, build methods and create/submit batches. 
Data Analysis details how to review TOF MS, Product Ion and IDA data using Analyst® TF, PeakView^TM and SCIEX OS software.

If required, you can find example data to use as practice for data processing in the Resources section on the left side. 

Chapters: Four (4)
Duration: 20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No

Course ID: eea72c13-fc3b-48a2-a4ea-b8ba16a89a75

This eLearning series is designed to teach a user how to use the Topaz^TM System and ClearCore^TM MD software for a basic quantitative workflow. It consists of four modules: Safety and Lab Requirements, Preparation, Optimization and Data Analysis.
Safety and Lab Requirements, and Preparation cover appropriate laboratory safety procedures, how to prepare the system, mobile phases, and samples.
Optimization covers compound and source optimization to create LC and MS methods, and how to acquire data.
Data Analysis details how to create a processing method, process and review data, and generate a report.

If required, you can find example data to use as practice for data processing in the Resources section on the left side. 

Chapters: Four (4)
Duration: 20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No 
Course ID:b9334a66-3851-45a8-aa0b-88bd3a837ac0

This self-paced training session covers the basics of mass spectrometry for new users including the uses of mass spectrometry, ion formation, mass analyzers and detectors. Learners interested in obtaining a PACE certificate and PACE accreditation for taking this course (equal to 0.5 PACE credit) must successfully complete the learning module and a brief online evaluation survey.

Chapters: 1
Duration: 33 minutes
Learner Level: Basic
Certificate Type: Completion
Pace Accreditation: Yes

This self-paced training session covers the basics of mass spectrometry for new users including the uses of mass spectrometry, ion formation, mass analyzers and detectors. Learners interested in obtaining a PACE certificate and PACE accreditation for taking this course (equal to 0.5 PACE credit) must successfully complete the learning module and a brief online evaluation survey.

Chapters: 1
Duration: 30 minutes
Learner Level: Basic
Certificate Type: Completion
Pace Accreditation: Yes

This is a general overview of the MetabolitePilot™ Software and its functionality, including use of the Compound Library, working with Biotransformations, setting up Processing Parameters, creating a Processing Batch in the Batch Workspace, reviewing the Results Workspace – including an overview of the Interpretation feature, and comparing data in the Correlation Workspace. Report generation and troubleshooting is also addressed.

Duration: approximately 28 minutes

This course is an introduction to microLC, and covers the following topics:

- Theory and benefits of microLC such as sampling efficiency, sensitivity improvements and solvent savings
- Modifying your method for microLC such as gradient conditions to use, appropriate injection volumes, how a trap and elute workflow can be beneficial, and best practices to achieve optimal robustness
- System requirements including the pump requirements, and appropriate tubing and electrospray emitter IDs

Duration: 10 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 73501550-6153-4295-b009-c04715c0e17e

This introductory eLearning course will give an overview of the functionality in SCIEX OS.

After completing this course, you should be:

• Comfortable navigating through SCIEX OS
• Understand the main function of each workspace in SCIEX OS
• Perform basic tasks in each workspace

Chapters: 1
Duration: 15 mins
Learner Level: Beginner
Certificate Type: Completion
PACE Accreditation: None
Course ID: 11391f0f-d0c8-413f-b508-ecd490f91d91

This course covers the theory and application of Time Of Flight mass spectrometry. Upon completion of the course, you will be able to:
- identify applications and benefits of Time of Flight (TOF) mass spectrometry
- explain the principles of TOF mass spectrometry at a high level
- understand the definition and application of accurate mass

Chapters: One (1)
Duration: 30 minutes
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No

This course will provide an introduction to triple quadrupole and linear ion trap theory, scans and operation on SCIEX mass spectrometers. Learners interested in obtaining a PACE certificate and PACE accreditation for taking this course (equal to one (1) PACE credit) must successfully complete the learning module and a brief online evaluation survey.

Chapters: 1
Duration: 40 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: Yes

This training session will provide an overview of the Jasper^TM HPLC system and how to use the system for sample analysis. 
After taking this course, you should be able to:
- Understand the components of the Jasper HPLC System
- Describe the LC flow path through the LC system
- Acquire data for samples
- Perform system tasks before and after acquisition

Chapters: One
Duration: 30 minutes
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 1ac184cc-3244-4b56-bc89-4949d0d85643

This module will inform you on the basic components and the workflow of the Lipidyzer^TM Platform. 

After taking this training course, you should be able to:
- Understand the design of the Lipidyzer Platform
- Have an understanding of the Lipidyzer Workflow
- Know the consumables needed to perform a direct infusion
- Have an understanding of the Lipidomics Workflow Software and its User Interface

Chapters: One (1)
Duration: 20 minutes
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 6a8d57b7-0e3e-451a-b404-c4665a56d9c8

This eLearning series is designed to teach you how to use the Echo MS® systems for a basic quantitative workflow. It consists of four modules: Safety and Lab Requirements, Mobile Phase and Standards Preparation, Data Acquisition, and Data Analysis. 
Safety and Lab Requirements, and Mobile Phase and Standards Preparation cover appropriate laboratory safety procedures, how to prepare the system, mobile phases, and samples. 
Data Acquisition covers creation of MS and AE methods and and how to acquire data. 
Data Analysis shows you how to create a processing method, process and review data, generate a report and perform auto-triggered data processing.

If required, you can find example data to use as practice for data processing in the Resources section on the left side. 

Chapters: Four (4)
Duration: 5-20 minutes for each online course
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: fd2a5568-6939-4216-9516-62e8987ee24c

This eLearning course will provide an overview of the Topaz^TM System and explain the theory of various scan types.
After taking this course, you should be able to:
- Understand the components of the Topaz System
- Describe the LC flow path through the LC system in all modes
- Explain how ions travel through the mass spectrometer
- Discuss the theory for MS and MS/MS scans

Chapters: One
Duration: 25 minutes
Course Level: Basic
Certificate Type: Completion
PACE Accreditation: No

Course ID: 2c29ab84-fa34-4ac0-8b66-c8809747765b

This course will introduce you to the different ionization techniques that can be performed using SCIEX ion sources, and important source and gas parameters for each ion source.  A guide on optimizing the source parameters for the OptiFlow® Pro Ion Source can be accessed in the Resources tab at the top of the course page. 

The course covers the following topics:
- ESI, APCI and APPI ionization
- Turbo V^TM, IonDrive^TM Turbo V, PhotoSpray®, DuoSpray^TM  OptiFlow^® Turbo V sources, and the OptiFlow® Pro Ion Source
- Important source parameters 

Duration: 20 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 06692297-47ce-4d9c-8bd6-0cd408ff6969

本培训课程将对Jasper HPLC系统做出概要介绍，并讲解如何使用该系统进行样品分析。

在学过本课程之后，你将能够：
- 认识Jasper HPLC系统的各单元

- 描述LC系统的流路
- 进行样品数据采集
- 并将能进行采集前的准备和采集后的收尾工作

章节：1
时长：30min
课程级别：基础
证书类型：Completion
PACE认证：否

本系列短视频是Jasper^TM HPLC 系统的维护演示，介绍了如何进行系列简单的仪器维护工作。

• 清洁柱温箱中的漏液
• 清洁泵漏液盘
• 拆卸自动进样器透明丙烯板
• 对进样口的清洁维护
• 冲洗进样针和定量环
• 去除冷凝水
• 更换保险丝
• 清洗单向阀
• 更换进样针密封垫
• 更换定量环

时长：每个视频1-4min
学员级别：基础
证书类型：无
PACE认证：无

在线学习系列课程为适用于SCIEX IVD 三重四极杆和QTRAP 的LC-MS/MS操作培训，包含四个单元：实验室安全须知、实验准备、方法优化、数据处理。在完成本系列课程之后，参与者将了解实验室安全规程，以及如何准备系统、流动相和样品，还将能够学会采集数据、浏览数据及使用MultiQuant MD软件生成报告。

单元数：4
时长：每单元20min
课程级别：基础
证书类型：Completion
PACE认证：否
Course ID: e57f9489-23bd-4c4f-8641-befa9153fc8c

本系列课程主要讲解SCIEX TripleTOF®系统的基础操作知识，共包含四个单元：实验室安全须知、实验准备、LC-MS/MS方法和数据处理。
实验室安全须知和实验准备单元包括适当的实验室安全程序，如何准备系统、流动相和样品。

LC-MS/MS方法单元包括如何调谐和校准SCIEX TripleTOF系统、构建方法和创建/提交样本批次。

数据处理单元详细介绍了如何使用Analyst®TF、PeakViewTM和SCIEX OS软件查看TOF MS数据、子离子数据和IDA数据。

课件屏幕左上角的资源文件夹中有可供数据处理操作参考的样本数据。

单元数：4
时长：20 min/单元
课程等级：基础
证书类别： Completion
PACE 认证：No

本培训系列主要内容为如何使用配备SCIEX OS 软件的X500R QTOF系统，课程包含四个单元：实验室安全须知、实验准备、LC-MS/MS分析方法和数据处理。完成课程后，学员将了解正确的安全防护、知道如何准备系统、流动相和样品。学员也将能够对X-系列 QTOF系统进行调谐和校准，并会建立方法和新建批次。最后的部分为如何查看TOF MS 和IDA数据。

单元：4
学习时长:20min/单元
课程级别：基础
PACE Accreditation: No

이 과정은 AB SCIEX QTRAP® 시스템 기술을 활용하여 LC-MS 기반 확인 화합물 스크리닝을 소개합니다. 이 과정을 이수하면 수집 방법을 만들고 배치를 제출하고 데이터를 처리하며 보고서를 만들 수 있습니다. 이 과정은 다음 주제로 구성되어 있습니다. - 화합물 스크리닝 프로세스 소개 - LC-MS/MS 시스템 개요 - IDA (Information Dependent Acquisition) 워크플로우 개요 - 수동으로 MRM-IDA 수집 방법 구축 및 IDA 마법사 사용 방법 - 배치 제출, 데이터 처리 및 화합물 스크리닝 보고서 생성 방법 장: 1 기간: 25~30 분 학습자 수준: 기초 인증 유형: 완료 PACE 승인: 없음

本在线学习系列课程为适用于SCIEX三重四极杆和QTRAP 的LC-MS/MS操作培训，包含四个单元：实验室安全须知、试验准备、优化与LC-MS/MS方法、数据处理。在完成本系列课程之后，参与者将了解实验室安全规程，以及如何准备系统、流动相和样品，还将能够优化方法、提交批次和使用MultiQuant软件生成结果列表与绘制标准曲线。

单元数：4
时长：每单元20min
课程级别：基础
证书类型：Completion
PACE认证：否

このコースでは、 SCIEX QTRAP^® システム テクノロジーを利用した LC-MS ベースの化合物同定スクリーニングについてご紹介します。このコースを修了すると、Acquisition Methodの作成、BatchのSubmit、データ解析、レポートの作成ができるようになります。

このコースでは、以下の内容をご紹介します。：
- LC-MSによる化合物同定スクリーニングのプロセスについて
- LC-MS/MS システムの概要
- IDA(Information Dependent Acquisition) ワークフローの概要 
- 手動およびIDA Method Wizard によるMRM-IDA Acquisition Methodの作成方法
- BatchのSubmit方法、データ解析について、化合物スクリーニングレポートの作成方法

チャプター数：1
所要時間：30分
学習レベル:中級
コース修了認定証： 有り
P.A.C.E. 認定対象: 対象外

本课程讲解了使用SCIEX QTRAP 系统进行的以LC-MS为基础的确证化合物筛查技术。学员能够通过课程学习到如何新建筛查采集方法、提交批次、进行数据处理和新建报告。

课程内容涵盖以下章节：
- 化合物筛查流程介绍
- LC-MS/MS 系统简介
- 信息依赖性采集（IDA）工作流程介绍
- 如何通过手动和IDA向导新建MRM-IDA 采集方法
-如何提交批次、处理数据和新建化合物筛查报告

模块分节：1
时长：25-30 min
学员水平：基础
证书类型： Completion
PACE 认证：No

This self-paced, online training session is designed to introduce current Linear Ion Trap instrument users to LightSight® 2.2 software. Functionality improvements including analog data analysis will be covered. Additionally, the training session will cover how to generate acquisition methods as well as process data. Lastly, the training session will go over how to generate and submit MS/MS methods and basic reporting functionality.

Duration: approximately 17 minutes

This training module is intended to train users how to use LipidView™ 1.2 software and develop processing methods to quickly and identify detected lipids and generate lipid profiles from collected data. Upon completion of Lipidview™ 1.2 software training, the user will be able to:

- Understand the overall design and functionality of the software and successfully interpret the results generated with the LipidViewTM software.

- Create and edit a processing method that is specific to the particular instrument and scan types used to acquire lipidomic data

- Review processed data using the multiple tools available in the software

- Generate customized reports detailing lipidomic results

- Export results for further processing by MarkerViewTM software

- Export methods to Analyst® software generated from discovery data

- Perform relative quantitation on multiple samples, using internal standards to normalize sample data

Duration: approximately 60 minutes

This self-paced training session provides an overview of MS3 quantitation also referred to as MRM cubed, used to solve difficult quantitation problems utilizing SCIEX QTRAP® systems. Users will learn the theory behind MRM3 as well as how to implement the workflow using proper Analyst® software parameters. This training session also provides a step-by-step guide to MRM3 method development.

Duration: approximately 17 minutes

In this course, we will be focusing on different types on column selectivity during method development.

After taking this course, the learner should be able to:
- understand the different factors affecting column selectivity
- know the differences between C18, C8, C4, phenyl and PFP type reverse phase columns
- understand polar analyte selectivity including normal phase, polar functional groups and HILIC selectivity.

Chapters: 1
Duration: approximately 12 minutes
Learner Level: Method Developer
Certificate Type: Completion
PACE Accreditation: No
Course ID: 2b4ea381-6eb6-4ac7-a268-c16ad0848948

This course introduces the subject of reverse phase mobile phases in method development. In this module, we will be focusing on the choice of solvents, mobile phase pH and buffers, isocratic vs. gradient systems and column temperature.

Chapters: 1
Duration: approximately 18 minutes
Learner Level: Method Developer
Certificate Type: Completion
PACE Accreditation: No
Course ID: 348dabcb-8b53-44b4-90e0-6364bb2b4610

This training module reviews the basics of mobile phase preparation and LC pump setup for mass spectrometry (MS) based analytical work using High Performance Liquid Chromatography (HPLC) systems. A brief overview of the components of an HPLC system is discussed and includes general tips on priming LC pumps. Special emphasis is given on proper use of MS grade reagents and measuring equipment. A short quiz tests the user’s knowledge after completion of the training module.

Duration: approximately 10 minutes

在此课程中以下内容将会逐一介绍：

• 软件关于Query的特点
• "Acceptance Query"和"MRM Ratio Query"的使用
• Query
• Query结果
• Query结果

单元数: 1
时长: 约15分钟
学习对象: 基础操作

The following topics are covered in this course:

•MultiQuant™ Software Query Overview
•Using Predefined Queries
•Create Simple Query
•Query Export

Chapters: 1
Duration: approximately 6 minutes
Learner Level: Basic
Certificate: None
PACE Accreditation: No
Course ID: 12242052-99f6-4c28-bf27-19e15a9f8918

In this course, the following topics are covered;

•MultiQuant™ Software and Regulated Environments

•Analyst Software Security Settings

•Audit Trail Manager

•Audit Trail Viewer and Review

•Audit Trail Searches

•Locking Results Table

•Audit Map Export

Duration: approximately 8 minutes

本课程是对LC-MS/MS 操作教程- 第四单元数据处理的中级扩展课程。

MultiQuant软件介绍中的工作流程与前面提到的LC-MS/MS课程中介绍的相同，并以此为基础，扩展每个使用者对SCIEX MultiQuant™软件界面的熟悉程度，并更了解该平台强大的分析能力。

完成本课程之后，学习者将能够:

- 在MultiQuant 软件中选择积分算法
- 使用MultiQuant Wizard 处理数据和生成结果表格
- 查看峰和更改积分参数
- 生成标准曲线、数据点图和分析统计表
- 通过Reporter工具生成报告

本在线自学课程中有一些交互部分，学习者可随时在课程中互动。

时长: 约 60 min

이 교육과정은 "LC-MS/MS 작동 시리즈 소개 – 4. 데이터 처리"의 기본 과정을 성공적으로 마친 교육생을 위한 중급 교육과정입니다.

MultiQuant 소프트웨어는 SCIEX의 MultiQuant ™ 소프트웨어 인터페이스에 대한 각 학습자의 지식을 확장하고 플랫폼의 견고한 분석 기능을 익히기 위한 기초로서, 앞에서 말한 LC-MS/MS 과정에서 소개 된 것과 동일한 워크 플로우를 따릅니다.

이 학습 모듈을 성공적으로 완료하면 학습자는 다음을 수행 할 수 있습니다.

- MultiQuant 소프트웨어 통합 알고리즘 선택
- MultiQuant Wizard를 사용하여 데이터를 처리하고 결과 테이블 생성
- 피크 검토 및 통합 매개 변수 변경
- 교정 곡선 생성, 플롯 메트릭스 작성 및 통계 분석
- Reporter Tool을 사용하여 보고서 작성하기

자기주도학습 모듈 중에는 학습자가 지시에 따라 코스 자료와 상호 작용할 준비를 해야하는 수많은 포인트가 있습니다.

---

소요 시간 : 약 60 분

이 강좌에서는 다음 주제를 설명합니다.

•MultiQuant™ 소프트웨어 쿼리 개요
•사전 정의된 쿼리 사용
•간단 쿼리 생성
•쿼리 내보내기

장: 1
시간: 약 10분
학습자 수준: 기초
인증: 없음
PACE 승인: 없음

The course introduces the Non-targeted Screening Workflow using the SCIEX X500R QTOF system with SCIEX OS. This is an advanced workflow course, and it is highly recommended that you complete the Introduction to LC-MS/MS on the X500R QTOF System eLearning series before taking this course. Upon completion of the course, learners will be able to create LC and MS methods for screening workflows,  acquire data, and process acquired data using Analytics. 

The course covers the following topics:
- Create LC and MS Methods
- Create a batch and acquire data
- Process data using the Non-targeted screening workflow in Analytics

Chapters: 1
Duration: 25 minutes
Learner Level: Advanced
Certificate Type: Completion
PACE Accreditation: No

Course ID: 81915288-a86d-46cd-a6d7-3fa9d50632d7

This course will show you how to use the OneOmics™ Suite in the SCIEX Cloud Platform for the ProteinPilot™Cloud Workflow for Proteomics. The topics covered are:
- Overview of the OneOmics Suite in the SCIEX Cloud Platform
- How to use CloudConnect for PeakView® software and upload your data files
- How to set up your experimental metadata with Experiment Manager to process your ProteinPilot data
- How to visualize the data using the Analytics and Browser applications

Chapters: 1
Duration: 20 minutes 
Course Certificate: Completion
PACE Accreditation: None

This course will show you how to perform SWATH quantification for Proteomics using the OneOmics™ Suite in the SCIEX Cloud Platform. The topics covered are:
- Overview of the OneOmics Suite in the SCIEX Cloud Platform
- How to use CloudConnect for PeakView® software and upload your data files
- How to set up your data processing jobs and define the experimental metadata using Experiment Manager
- Data processing using the Extractor and Assembler applications
- How to visualize the data using the Analytics and Browser applications

Chapters: 1
Duration: 20 minutes 
Course Certificate: Completion
PACE Accreditation: None

This course will show you how to visualize SWATH® results for Proteomics and Metabolomics using the OneOmics™ Suite in the SCIEX Cloud Platform. The topics covered are:
- Overview of the OneOmics Suite in the SCIEX Cloud Platform
- How to use the MarkerView™ and Review applications to visualize your results
- Show an example of how to process and visualize a full Multi-Omics data set using the tools in the OneOmics Suite

Chapters: 1
Duration: 20 minutes 
Course Certificate: Completion
PACE Accreditation: None

This course introduces the SCIEX X500B QTOF System and BioPharmaView^TM software, and demonstrates the workflow for Peptide Mapping analysis. 

After taking this course you should be able to:
- Understand the basics of Peptide Mapping analysis
- Create LC and MS methods in SCIEX OS
- Submit a batch in SCIEX OS
- Process peptide mapping data using BioPharmaView software

Chapters: 1 
Duration: 20 minutes
Learner Level: Basic 
Certificate Type: Completion
PACE Accreditation: No

This is the first training module in Peptide Quantitation eLearning Series.

Upon completion of this eLearning module you will be able to identify major components of peptide backbone structure and conventional amino acids, predict general properties and charge state from peptide sequence, understand mode of peptide fragmentation in a quadrupole, associated peptide MS/MS fragments with a peptide sequence, and understand use of heavy isotope labeled internal standard peptides.

Chapters: 1
Duration: 20 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: No

This is the second training module in Peptide Quantitation eLearning Series. Upon completion of this eLearning module, you will be able to understand two major groups of Peptide Quant experiments; identify major ‘rules’ for picking MRM transitions for peptides;understand infusion based peptide MRM optimization methods; utilize Skyline software to predict potential fragment ions for any peptide sequence; and utilize Skyline software for on-column peptide MRM screening and optimization.

Chapters: 1
Duration: 30 minutes
Learner Level: Basic
Certificate Type: None
PACE Accreditation: No

This is the third training module in Peptide Quant eLearning Series. Upon completion of this training, you will understand basic principles behind protein precipitation methods; Understand isotope labeled internal standard peptides and their use in each sample prep protocol;Identify the different types of solid phase extractions methods, and determine proper conditions for enrichment of a specific peptide analyte; Be able to calculate matrix effects and recovery of extraction procedures; Understand peptide immunoaffinity enrichment strategies.

Chapters: 1
Duration: 30 minutes
Learner Level: Basic
Certificate Type: None
PACE Accreditation: No

This is the 4th training module in Peptide Quant eLearning Series. After taking this training course, the user should be able to: Understand the use of isotope labeled internal standard proteins and peptides and their use in each sample prep protocol; Understand basic principles behind size exclusion filtration methods; Identify different types of immunoaffinity captures methods, including depletion and enrichment strategies; Be able to apply generic protein precipitation and resolubilization protocols; Understand basic protein protease digestion methods for the cleavage of a protein target into peptides; Be able to calculate digestion efficiency and recovery of extraction preparation procedures.

Chapters: 1
Duration: 30 minutes
Learner Level: Basic
Certificate Type: None
PACE Accreditation: No

This is the fifth training module in Peptide Quantitation eLearning Series. Upon completion of this eLearning module you will be able to: Identify challenges associated with peptide quantitation such as poor fragmentation, high background, and endogenous interferences; Understand the use of an MRM3 method to selectively remove background and interferences for peptide quant; Understand the use of the SelexION™ differential mobility device to separate peptide analytes from endogenous interferences, even with poor fragmenting peptides.

Chapters: 1
Duration: 20 minutes
Learner Level: Basic
Certificate Type: None
PACE Accreditation: No

This course introduces the subject of how to process QTRAP data using the MasterView™ software. Upon completion of the course, learners will be able to successfully create acquisition and processing methods, and review their processing results in MasterView™.

After taking this training course, the user should be able to:
- Create an Scheduled MRM™-IDA-MS/MS acquisition method in Analyst® software
- Import MS/MS libraries into MasterView™ software
- Create a processing method for QTRAP® MS/MS library searching in MasterView™ software
- Process Scheduled MRM™-IDA-MS/MS data in MasterView™ software
- Review and filter results
- Report results and export processing parameters for further processing in MultiQuant™ software

Chapters: 1
Duration: 25 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: No
Course ID: 2d4b24e4-313f-47d5-b3b3-cc04b21a6e79

During this eLearning course, you will be presented with current challenges in the analysis of lipids by mass spectrometry; Introduced to the MS/MS ALL workflow and given step-by-step instructions for its implementation;Introduced to data processing strategies to deal with the large data arrays generated during lipidomics analysis.

This course covers the Quantitation and targeted identification workflow with SWATH Acquisition using the SCIEX X500R QTOF system. This is an advanced course, and it is recommended that learners first complete the "Introduction to LC-MS/MS" course prior to beginning this module. Upon completion of the course, learners will be able to create an LC and MS SWATH acquisition method, acquire and process data.

The course covers the following topics:
- What is SWATH Acquisition?
- Creating a LC Method in SCIEX OS
- Using the SWATH Variable Window Calculator and creating an MS Method in SCIEX OS
- Acquiring SWATH data
- Processing TOF-MS and TOF-MS/MS data using Analytics

Chapters: 1
Duration: 25 minutes
Learner Level: Advanced
Certificate Type: Completion
PACE Accreditation: No

This eLearning series focuses on quantitative data processing using the Analytics workspace in SCIEX OS software for all SCIEX instruments (excluding MD instruments). This entire eLearning series consists of 3 modules : Algorithms and flagging, Create method and results, and Review and report results. 

After completing this eLearning series of modules, you should be able to:
- Understand the different types of integration algorithms and their parameters
- Explain the pre-defined flagging rules for concentration, accuracy, integration and ion ratios
- Create a processing method
- Create results
- Perform data review
- Export and report results

Chapters: 3
Duration: 10-30 mins (for each module)
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None

This series of quick maintenance videos covers the SCIEX triple quadrupole and QTRAP systems. You will become familiar with performing simple maintenance tasks for your system.

General System Maintenance Videos:
- Basic computer maintenance

Ion Source Maintenance Videos:
- Removing and installing the ion source
- Removing and installing the probe
- Removing ion source covers
- Replacing the metal turbo heater on the Turbo V ion source with external covers
- Replacing the turbo heater on the IonDrive Turbo V ion source with external covers
- Replacing the ceramic turbo heater on the Turbo V ion source without external covers
- ESI probe maintenance and troubleshooting
- APCI corona discharge needle replacement

Chapters: 9
Duration: 30 minutes
Course Level: Advanced
Certificate Type: None
PACE Accreditation: No

This series of short maintenance videos focuses on the SCIEX X500 QTOF System mass spectrometers, and will cover how to perform simple instrument maintenance. Upon completion of this course, uses will be able to:

-- Perform system startup and shutdown
-- Replace the twin electrodes
-- Perform front end cleaning of the mass spectrometer
-- Refill and replace the CDS bottles

Chapters: 5
Duration: 20 minutes
Course Level: Basic
Certificate Type: None
PACE Accreditation: No

Course ID#: 7de75134-4ec2-471b-8ad6-e98f8e0f2640

In this course, the Resolution equation and its components will be described and how each component affects resolution.

Duration: approximately 15 minutes

This is the Customer Familiarization course for SCIEX 4500 and 5500 series mass spectrometers. You will be familiarized with the software and hardware overview, how to tune and calibrate the instrument, run a sample batch, and perform simple maintenance.

This course is an introduction to the SCIEX Empower™ Driver that is used on SCIEX CE instrumentation. The Empower driver is a software interface that gives users direct control of their PA 800 Plus Pharmaceutical Analysis System in the Empower 3 Chromatography Data System or CDS.

This course will cover the following topics:

• Overview of the SCIEX Empower Driver
• Features and new updates of the Empower Driver related to detectors, creating methods and data processing
• Video demonstration of using the features of the Empower Driver

Chapters: 1
Duration: 15 minutes
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None

This course is an overview of the SCIEX Fast Glycan labelling and analysis technology. It covers how to perform sample preparation using the SCIEX Fast Glycan Labeling and Analysis kit, and obtain separation of the glycans by capillary electrophoresis (CE) on the PA 800 Plus System.

After taking this course, you should be able to:
- Understand the importance of glycan analysis
- Perform sample preparation using the SCIEX Fast Glycan Labeling and Analysis kit
- Use the PA 800 Plus System to perform data analysis
- Troubleshoot common issues that occur when using the Fast Glycan Labeling and Analysis application

Duration: 40 mins
Learner Level: Basic
Certificate Type: Completion
PACE Accreditation: None
Course ID: 10a2a1f9-5cc2-4a8a-8b40-28a7392f0bae

This course explains an overview of the source exhaust for your mass spectrometer, and how it works. It explains the typical symptoms of source exhaust failure using both visual and audible clues, and details basic troubleshooting and maintenance tasks to keep your source exhaust working.

Chapters: 1
Duration: 10 minutes
Learner Level: Basic
Certificate Type: None
PACE Accreditation: No