Course Catalog

You can browse, filter, or search our extensive list of training offerings. Choose from over 100 self-paced eLearnings or search for an instructor-led course near you. Once you select the course you want, you will be directed to SCIEX Now Learning Hub for enrollment (login required).

Agilent Lab Advisor software View Course

  • Duration: 20 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course will introduce you to the Agilent Lab Advisor Software and give you an overview of the software and its functionality.

After taking this course, you should be able to:
  • Add and connect a system
  • Configure your system
  • Update the firmware
  • View logs, messages and results for your system
  • Perform Service and Diagnostics procedures
  • Control your system using Lab Advisor software
  • View and update EMF counters
  • Create reports

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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Analyst Software - Acquire View Course

  • Duration: 15 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:
This training courses focuses on method creation and data acquisition using Analyst and Analyst MD software. It covers the following topics:
  • Creation of basic and advanced acquisition methods such as scheduled-ionization, Scheduled MRM (sMRM), multiple periods and IDA experiments
  • Creation of an acquisition batch
  • Submission of the batch to the queue
  • Starting data acquisition
Chapters: 1
Certificate Type: Completion
PACE Accreditation: No
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Analyst Software - Compound and Source Optimization View Course

  • Duration: 25 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This self-paced training session focuses on the basics of using Analyst and Analyst MD software for compound and source/gas optimization for triple quadrupole and QTRAP instrument systems. Manual compound and source/gas optimization will be shown. Additionally, automated compound optimization by Infusion and automated source/gas optimization by Flow Injection Analysis will be covered using the Compound Optimization function.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Analyst Software - Library Search View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This self-paced training session covers the basics of the Library Search function. Topics include but not limited to: where library databases are stored, how to connect to a library, how to add and edit compounds to a library, how to show the contents of a library, and how to search unknowns against a library.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Analyst Software - Quantitation View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This self paced presentation will take Analyst software users through the quantitative work flow of MRM data generated on SCIEX Triple Quad and QTRAP System instruments. Users will learn how to choose the integration algorithms, use the Quantitation Wizard to integrate LC-MS/MS data to perform linear regression and generate reports through the Analyst Software Reporter tool.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Analyst Software - Tune and Calibrate View Course

  • Duration: 40 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This self paced training session focuses on the basics of using Analyst and Analyst MD software for Resolution and Calibration for triple quadrupole and QTRAP instrument systems. Topics included are:

  • Resolution Optimization
  • Reviewing Resolution Peak Spectra
  • Mass Calibration from Spectrum
  • Mass Calibration Report and the Mass Calibration Result.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Analyst software - Explore mode View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:
This course explains how to use the Explore mode of Analyst and Analyst MD software to process data. The course covers the following topics: 
  • Explore mode overview
  • Adding text
  • Overlaying data and extracting spectra/XICs
  • Examples of processing functions
  • Fragment interpretation tool
  • Using scripts

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Analyst 软件数据库搜索功能(Analyst Software - Library Search) View Course

  • Duration: 30 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

本在线学习课程主要介绍数据库搜索的基础功能。内容包含但不限于:数据库的储存位置、如何关联数据库、如何在数据库中添加和编辑化合物、如何展示数据库内容,以及如何在数据库中搜索未知物。

时长:约8 min

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Analyst 软件数据浏览模块概览(Analyst Software - Explore) View Course

  • Duration: 15 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

该自学培训课程介绍了在AnalystAnalyst MD软件的Explore模块下处理数据文件的基本功能。主题包括但不限于:打开数据文件、缩放谱图、设置背景区域、生成质谱图、生成提取离子色谱图、数据平滑、谱图标记、处理插件和片段解析工具。

时长:  约 15 min

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Analyst 软件的调谐与校准 View Course

  • Duration: 35 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

本自控速培训的主要内容为使用Analyst软件为三重四极杆和线性离子阱系统进行验证和校准。包括根据指标验证和校准单位分辨率、质量数偏移、峰值强度,也介绍了如何检查校正结果。

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Analyst软件化合物和离子源优化操作培训 View Course

  • Duration: 25 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

本自助学习课程主要讲解了在四极杆和线性离子阱仪器上使用Analyst软件进行source/gas优化的过程。手动化合物和source/gas优化都将介绍到。另外,课程还概况了使用针泵和流动注射分析的自动优化功能。

时长:约25min

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Analyst软件的采集功能 View Course

  • Duration: 15 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

本自助学习课程主要讲解如何新建基础的采集方法,包括新建采集批次、向序列中提交批次和运行采集等步骤。

学习时长:15分钟左右

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Application tips for optimal sensitivity View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This self-paced, online training session will provide Triple Quadrupole and QTRAP System users with some practical suggestions to improve the sensitivity of their experiments.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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AutoPeak Essentials View Course

  • Duration: 15 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This course covers an overview of the AutoPeak integration algorithm available in the Analytics workspace of SCIEX OS software.

After taking this course, you should be able to:
  • understand the theory of the AutoPeak integration algorithm
  • create a processing method using AutoPeak
  • describe basic and advanced AutoPeak integration parameters
  • explain how saturation correction is applied
  • create results and review data using AutoPeak

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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Automated Carryover Monitoring for the MPX 2.0 High Throughput System View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This course will introduce you to the MPX 2.0 High Throughput System and how automated carryover monitoring works.

After taking this course, you should be able to:
  • Describe how multiplexing works
  • Explain the automated carryover monitoring process
  • Create acquisition and quantitation methods
  • Create and submit a batch
  • Review the batch in the MPX software

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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BioPharmaView Software 3.0 for MAM Workflow View Course

  • Duration: 15 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course will show you how to process data for a structured Multiple Attribute Methodology (MAM) workflow using BioPharmaView TM Software 3.0.

This course covers the following topics:
  • Overview of BioPharmaView Software 3.0
  • What is MAM?
  • Benefits of using a MAM approach 
  • Step by Step Data Processing for MAM Workflow using BioPharmaView Software

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Biologics Workflows Using SCIEX TripleTOF Mass Spectrometer View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

In this course, we will walk you through the intact protein molecular weight workflow, peptide mapping and quantitation, MS/MS ALL workflow for host cell protein detection, and the utilization of CESI as an additional separation strategy using the TripleTOF system.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Changing the roughing pump oil View Course

  • Duration: 10 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:
This course demonstrates how to change the roughing pump oil for your SCIEX mass spectrometer, and is applicable for the following systems:
  • Triple quadrupole and QTRAP systems (RUO and MD)
  • X500 QTOF series systems
  • TripleTOF systems
It will cover the following topics:
  • Overview of why you should change the roughing pump oil and when to change it
  • Tools and materials needed
  • Steps involved in the oil change process

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Data Acquisition and Processing using the Lipidyzer Platform View Course

  • Duration: 10-20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course is comprised of 4 chapters that cover the data acquisition and processing workflow on the Lipidyzer TM Platform. It is intended that you view each of these chapters in the following sequence shown. You will achieve a certificate once you have successfully completed all four chapters of this course.

Chapter 1: Performance Tests teaches you how to run the performance tests for the Lipidyzer Platform in order to ensure that settings for each lipid class are correct and to verify that the instrument is performing to specification.
Chapter 2: Batch Generation teaches you how to register an internal kit and then create a Lipidyzer Workflow.
Chapter 3: Sample Extraction tells you how to create an internal standard superpool mix and complete a successful Bligh & Dyer Lipid extraction.  
Chapter 4: Results shows you how to view and report your results from your samples using the projects and batches functions in the Lipidomics software. 

Chapters: Four (4)
Certificate Type: Completion
PACE Accreditation: No

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Echo MS 系统介绍 (Introduction to the Echo MS ) View Course

  • Duration: 15 mins
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

本在线课程是对Echo MS系统的概览介绍。课程将讲授以下内容:

  • Echo MS 系统的部件和原理
  • 如何通过 SCIEX OS 配置Echo MS 系统
  • 建立方法
  • 使用直接设备控制( Direct Device Control )
  • 执行维护工作

单元数:1
证书类别:Completion
PACE 认证:None

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Getting the Most Out of the Scheduled MRM Algorithm View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This course covers the theory of the Scheduled MRM algorithm in Analyst and Analyst MD software, and how to create methods using the algorithm. It also covers enhancements to the algorithm in Analyst® software version 1.7.

After taking this course, you should be able to:
  • Understand how the Scheduled MRM algorithm works
  • Create a method using basic and advanced parameters
  • Describe some rules to follow when creating a method
  • Optimize your method using the sMRM Calculator script

Chapters:1 
Certificate Type: Completion
PACE Accreditation: None

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High-Resolution MRM (MRMHR) Workflow Using SCIEX QTOF and ZenoTOF Systems View Course

  • Duration: 25 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The course introduces the high-resolution MRM workflow using SCIEX QTOF and ZenoTOF Systems with SCIEX OS. This is an advanced workflow course, and it is recommended that you complete the LC-MS/MS Operation Series for the X500R QTOF system eLearning course before taking this course. Upon completion of the course, learners will be able to build an optimized MS method, submit a batch and process data.

The course covers the following topics:
  • Guided MRM HR compound optimization
  • TOF MSMS method editor
  • Build a batch and run scheduled MRM HR
  • Process data using the quantitation, and quantitation and targeted identification workflows

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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IDA method creation using SCIEX QTRAP systems View Course

  • Duration: 40 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

The purpose of this presentation is to introduce novice users of SCIEX QTRAP instruments to the subject of linear ion trap method creation. It is intended to introduce topics which the user might need to be aware of during method development.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Installing the OptiFlow interface on TripleTOF systems View Course

  • Duration: 4 mins
  • Language: English
  • Type: Premium, How-to Videos
  • Course Level: Intermediate
  • Rating:

This eLearning course will demonstrate how to remove the OptiFlow interface from your TripleTOF system and switch between nanoflow and microflow or high flow.

After completing this course, you should be able to:
  • Remove the nano ion source and install a high or microflow ion source
  • Remove the OptiFlow interface and install the high flow curtain plate
  • Install the microflow electrode in the probe for the Turbo V ion source

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Intact Biologics Analysis Using SCIEX X500B QTOF System and BioPharmaView Software View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

In this course, we introduce the SCIEX X500B QTOF System and BioPharmaView software, and demonstrate the workflow for Intact Biologics analysis.

After taking this course, you should be able to:

  • Understand the basics of Intact Biologics analysis
  • Be familiar with the hardware and software features
  • Create LC and MS methods to acquire data in SCIEX OS
  • Submit a batch
  • Process Intact Biologics data using BioPharmaView software

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Introduction to LC-MS compound screening View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The course introduces the subject of LC-MS based confirmation compound screening utilizing SCIEX QTRAP systems technology. Upon completion of the course, learners will be able to create an acquisition method, submit a batch, process data, and create a report.

The course covers the following topics:
  • Introduction to the compound screening process
  • Overview of an LC-MS/MS system
  • Overview of the Information Dependent Acquisition (IDA) workflow
  • How to build an MRM-IDA acquisition method manually and using the IDA Wizard
  • How to submit a batch, process data and creating a compound screening report

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Introduction to PeakView Software and its Use in Met ID Data Processing View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

After taking this training course, you will be able to perform basic operations in PeakView Software and use some of the more advanced functions such as FormulaFinder and Fragment Pane for the Met ID workflow.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Introduction to SelexION Differential Mobility Separation Technology View Course

  • Duration: 25 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

This course is intended to cover the theory of Differential Mobility Separation (DMS) Technology and how to use the SelexION device with your mass spectrometer.

After taking this course, you should be able to:
  • Understand the theory of DMS and DMS method parameters
  • Install the SelexION device and equilibrate the system before use
  • Optimize DMS method parameters for your analytes
  • Perform basic maintenance tasks
Refer to the Resources section on the left to view additional documents:
  • Workflows for Method Development and Routine use
  • Cleaning Procedures
  • Installation and Removal Procedures

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Introduction to the Echo MS System View Course

  • Duration: 15 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This eLearning course will give an overview of the Echo MS System. It will cover the following topics:

  • Components and theory of the Echo MS System
  • How to configure the Echo MS System using SCIEX OS
  • Creating MS, AE and processing methods and batches
  • Using Direct Device Control
  • Maintenance tasks

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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Introduction to the OptiFlow Quant Solution for MicroLC View Course

  • Duration: 25 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This eLearning series covers the OptiFlow Quant Solution for MicroLC. It applies to selected triple quadrupole, QTRAP® and TripleTOF® systems coupled with an OptiFlow® Turbo V ion source and the M5 MicroLC system. The series consists of a two part course:

  • System Overview
  • System Setup and Use
After completing this course, you should be able to:
  • Describe the different components in the OptiFlow Quant Solution
  • Setup and use the OptiFlow Turbo V ion source
  • Setup the system for the M5 MicroLC system
  • Describe the trap-elute workflow
  • Define mobile phases, purge and flush the M5 MicroLC system
  • Create autosampler methods using the Analyst Device Driver
  • Create acquisition methods for direct injection and trap-elute experiments
  • Understand best practices and maintenance of the M5 MicroLC system

Chapters: 2
Certificate Type: Completion
PACE Accreditation: None

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Ionization techniques for SCIEX sources View Course

  • Duration: 15 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course will introduce you to the different ionization techniques that can be performed using SCIEX ion sources, and important source and gas parameters for each ion source.  A guide on optimizing the source parameters for the OptiFlow Pro ion source can be accessed in the Resources tab at the top of the course page. 

The course covers the following topics:
  • ESI, APCI and APPI ionization
  • Turbo V,  IonDrive Turbo V, PhotoSpray, DuoSpray, OptiFlow Turbo V sources, and the OptiFlow Pro ion sources
  • Important source parameters 

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Jasper HPLC 系统维护系列视频 View Course

  • Duration: 5 minutes
  • Language: Chinese
  • Type: Premium, How-to Videos
  • Course Level: Advanced
  • Rating:

本系列短视频是JasperTM HPLC 系统的维护演示,介绍了如何进行系列简单的仪器维护工作。

  • 清洁柱温箱中的漏液
  • 清洁泵漏液盘
  • 拆卸自动进样器透明丙烯板
  • 对进样口的清洁维护
  • 冲洗进样针和定量环
  • 去除冷凝水
  • 更换保险丝
  • 清洗单向阀
  • 更换进样针密封垫
  • 更换定量环

证书类型:无
PACE认证:无

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LC-MS化合物筛查入门 View Course

  • Duration: 30 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

本课程讲解了使用SCIEX QTRAP 系统进行的以LC-MS为基础的确证化合物筛查技术。学员能够通过课程学习到如何新建筛查采集方法、提交批次、进行数据处理和新建报告。

课程内容涵盖以下章节:
- 化合物筛查流程介绍
- LC-MS/MS 系统简介
- 信息依赖性采集(IDA)工作流程介绍
- 如何通过手动和IDA向导新建MRM-IDA 采集方法
-如何提交批次、处理数据和新建化合物筛查报告

模块分节:1
证书类型: Completion
PACE 认证:No

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LightSight 2.2 Software for Metabolite Identification View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This self-paced, online training session is designed to introduce current Linear Ion Trap instrument users to LightSight 2.2 software. Functionality improvements including analog data analysis will be covered. Additionally, the training session will cover how to generate acquisition methods as well as process data. Lastly, the training session will go over how to generate and submit MS/MS methods and basic reporting functionality.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Lipid Analysis by Differential Ion Mobility Spectrometry - 1 General Overview View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

During this course, you will learn about the fundamental challenge of lipidomics analysis, namely isobaric interference, what SelexION® Technology is and how it works, the development of DMS as a tool for lipid analysis by mass spectrometry and finally see how SelexION® Technology has been used to solve difficult analytical problems in lipidomics.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Lipid Analysis by Differential Ion Mobility Spectrometry - 2 Hardware Components and Parameter Settings View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The overall objective of this eLearning module is to describe in detail the different aspects of the SelexION® Technology device, including the hardware components and parameter settings. During this course, you will learn about the procedures to place the DMS cell onto the mass spectrometer and equilibrate the instrument. Additionally, the different parameter settings that control the function of the SelexION® Technology device will be introduced and discussed in terms of how they affect experimental results.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Lipid Analysis by Differential Ion Mobility Spectrometry - 3 Qualitative Analysis View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The overall objective of this eLearning module is to describe how to use the SelexION® Technology device during qualitative analysis and to improve the qualitative characterization of lipids. During this course, you will learn how to develop qualitative analytical assays using the SelexION® Technology device on QTRAP® or Triple Quadrupole Systems or Triple TOF® Instruments. The sections in this module include COV optimization strategies on SCIEX instrument platforms, example data from qualitative lipid analysis and online resources.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Lipid Analysis by Differential Ion Mobility Spectrometry - 4 Quantitative Analysis View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The overall objective of this eLearning module is to describe how to use the SelexION® device during quantitative analysis of lipids. The use of SelexION® Technology (differential ion mobility spectrometry, or DMS) in the study of lipidomics greatly improves the quantitative analysis of lipids by lowering the chromatographic baseline (i.e., noise) and minimizing isobaric interference. In this course, the steps needed to develop quantitative assays using DMS will be described on SCIEX Triple™ Quadrupole Instruments/QTRAP® System.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Lipid Analysis by Differential Ion Mobility Spectrometry - 5 Maintenance & Troubleshooting View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The overall objective of this eLearning module is to describe routine maintenance of the SelexION® Technology device during the analysis of lipids. Regular cleaning of the DMS cell is suggested to maintain its robustness and performance. In this course, the procedures to clean the hardware are presented, and a troubleshooting guide is provided.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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LipidView 1.2 Software Training View Course

  • Duration: 1 hour
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This training module is intended to train users how to use LipidView 1.2 software and develop processing methods to quickly and identify detected lipids and generate lipid profiles from collected data. 

Upon completion of LipidView 1.2 software training, you will be able to:
  • Understand the overall design and functionality of the software and successfully interpret the results generated with the LipidView software.
  • Create and edit a processing method that is specific to the particular instrument and scan types used to acquire lipidomic data
  • Review processed data using the multiple tools available in the software
  • Generate customized reports detailing lipidomic results
  • Export results for further processing by MarkerView software
  • Export methods to Analyst software generated from discovery data
  • Perform relative quantitation on multiple samples, using internal standards to normalize sample data

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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MRM Cubed Application for Quantitation View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This self-paced training session provides an overview of MS3 quantitation also referred to as MRM cubed, used to solve difficult quantitation problems utilizing SCIEX QTRAP® systems. Users will learn the theory behind MRM3 as well as how to implement the workflow using proper Analyst® software parameters. This training session also provides a step-by-step guide to MRM3 method development.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Maintenance for the Lipidyzer Platform View Course

  • Duration: 10 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This module will inform you how to perform basic maintenance for the Lipidyzer Platform, and includes some FAQs.

After taking this module you should be able to….
  •  Install all hardware parts of the SelexION® device
  •  Clean and reassemble the DMS cell
  •   Check that the direct infusion spray is stable

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Method Development - Column Selectivity View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

In this course, we will be focusing on different types on column selectivity during method development.
After taking this course, the learner should be able to:

  • understand the different factors affecting column selectivity
  • know the differences between C18, C8, C4, phenyl and PFP type reverse phase columns
  • understand polar analyte selectivity including normal phase, polar functional groups and HILIC selectivity.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Method Development - Mobile Phase View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course introduces the subject of reverse phase mobile phases in method development. In this module, we will be focusing on the choice of solvents, mobile phase pH and buffers, isocratic vs. gradient systems and column temperature.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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MultiQuant 2.1 的query(查询)功能 View Course

  • Duration: 15 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

在此课程中以下内容将会逐一介绍:
  • 软件关于Query的特点
  • "Acceptance Query"和"MRM Ratio Query"的使用
  • Query
  • Query结果
  • Query结果

单元数: 1
学习对象: 基础操作

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MultiQuant MD 软件操作介绍(Working With MultiQuant MD) View Course

  • Duration: 25 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

本课程主要介绍使用MultiQuant MD软件进行定量。在完成本课程后,学习者将能够处理数据、审查结果并导出数据。

本课程将涵盖以下内容:

-新建处理方法和设置默认方法参数

-处理数据并查看结果表

-导出和报告数据

-使用 Audit Trail 查看器查看事件审计结果

章节: 1
证书类别: 结课
PACE 认证: No

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MultiQuant Software 2.1 Audit Trail Security View Course

  • Duration: 10 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

In this course, the following topics are covered;

  • MultiQuant Software and Regulated Environments
  • Analyst Software Security Settings
  • Audit Trail Manager
  • Audit Trail Viewer and Review
  • Audit Trail Searches
  • Locking Results Table
  • Audit Map Export

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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MultiQuant Software Queries View Course

  • Duration: 10 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The following topics are covered in this course:

•MultiQuant Software Query Overview
•Using Predefined Queries
•Create Simple Query
•Query Export

Chapters: 1
Certificate: None
PACE Accreditation: No

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MultiQuant 软件操作介绍(Working With MultiQuant Software) View Course

  • Duration: 60 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

本课程是对LC-MS/MS 操作教程- 第四单元数据处理的中级扩展课程。

MultiQuant软件介绍中的工作流程与前面提到的LC-MS/MS课程中介绍的相同,并以此为基础,扩展每个使用者对SCIEX MultiQuant软件界面的熟悉程度,并更了解该平台强大的分析能力。

完成本课程之后,学习者将能够:

- 在MultiQuant 软件中选择积分算法
- 使用MultiQuant Wizard 处理数据和生成结果表格
- 查看峰和更改积分参数
- 生成标准曲线、数据点图和分析统计表
- 通过Reporter工具生成报告

本在线自学课程中有一些交互部分,学习者可随时在课程中互动。

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Non-targeted screening data processing using SCIEX OS software View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This eLearning course focuses on data processing using the non-targeted screening workflow in the Analytics workspace of SCIEX OS software. This is applicable to all SCIEX instruments (excluding MD instruments).  

After completing this course, you should be able to:
  • Describe the non-targeted workflow
  • Understand how library searching and formula finding can be used in data review
  • Create a processing method
  • Create results
  • Perform data review
  • Export data and create a report

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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Non-targeted screening workflow for SCIEX QTOF and ZenoTOF systems View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The course introduces the non-targeted screening workflow using SCIEX QTOF and ZenoTOF systems with SCIEX OS. This is an advanced workflow course, and it is highly recommended that you complete the Introduction to LC-MS/MS operation series for the SCIEX QTOF and ZenoTOF systems eLearning series before taking this course. Upon completion of the course, learners will be able to create LC and MS methods for screening workflows,  acquire data, and process acquired data using Analytics. 

The course covers the following topics:
  • Create LC and MS methods
  • Create a batch and acquire data
  • Process data using the Non-targeted screening workflow in Analytics

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Peptide Mapping Analysis Using SCIEX X500B QTOF System and BioPharmaView Software View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course introduces the SCIEX X500B QTOF System and BioPharmaView software, and demonstrates the workflow for Peptide Mapping analysis. 

After taking this course you should be able to:

  • Understand the basics of Peptide Mapping analysis
  • Create LC and MS methods in SCIEX OS
  • Submit a batch in SCIEX OS
  • Process peptide mapping data using BioPharmaView software
Chapters:1 

Certificate Type: Completion
PACE Accreditation: No

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Peptide Quant Series - 1. Peptide Structure and Fragmentation View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This is the first training module in Peptide Quantitation eLearning Series. Upon completion of this eLearning module you will be able to identify major components of peptide backbone structure and conventional amino acids, predict general properties and charge state from peptide sequence, understand mode of peptide fragmentation in a quadrupole, associated peptide MS/MS fragments with a peptide sequence, and understand use of heavy isotope labeled internal standard peptides.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Peptide Quant Series - 2. Introduction to Peptide MRM Development View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This is the second training module in Peptide Quantitation eLearning Series. Upon completion of this eLearning module, you will be able to understand two major groups of Peptide Quant experiments; identify major ‘rules’ for picking MRM transitions for peptides;understand infusion based peptide MRM optimization methods; utilize Skyline software to predict potential fragment ions for any peptide sequence; and utilize Skyline software for on-column peptide MRM screening and optimization.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Peptide Quant Series - 3. Introduction to Sample Preparation Techniques for Peptides View Course

  • Duration: 40 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:
This is the third training module in Peptide Quant eLearning Series. Upon completion of this training, you will:
  • Understand basic principles behind protein precipitation methods
  • Understand isotope labeled internal standard peptides and their use in each sample prep protocol
  • Identify the different types of solid phase extractions methods, and determine proper conditions for enrichment of a specific peptide analyte
  • Be able to calculate matrix effects and recovery of extraction procedures; Understand peptide immunoaffinity enrichment strategies.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Peptide Quant Series - 4. Introduction to Sample Preparation Techniques for Protein Targets View Course

  • Duration: 40 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:
This is the 4th training module in Peptide Quant eLearning Series. After taking this training course, the user should be able to:
  • Understand the use of isotope labeled internal standard proteins and peptides and their use in each sample prep protocol
  • Understand basic principles behind size exclusion filtration methods
  • Identify different types of immunoaffinity captures methods, including depletion and enrichment strategies
  • Be able to apply generic protein precipitation and resolubilization protocols
  • Understand basic protein protease digestion methods for the cleavage of a protein target into peptides
  • Be able to calculate digestion efficiency and recovery of extraction preparation procedures.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Peptide Quant Series - 5. Advanced MS Selectivity for Peptide Quantitation on QTRAP Systems View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This is the fifth training module in Peptide Quantitation eLearning Series. Upon completion of this eLearning module you will be able to:

  • Identify challenges associated with peptide quantitation such as poor fragmentation, high background, and endogenous interferences
  • Understand the use of an MRM3 method to selectively remove background and interferences for peptide quant
  • Understand the use of the SelexION differential mobility device to separate peptide analytes from endogenous interferences, even with poor fragmenting peptides.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Processing of QTRAP MS/MS Data in MasterView Software for Identification View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course introduces the subject of how to process QTRAP data using the MasterView software. Upon completion of the course, you will be able to successfully create acquisition and processing methods, and review your processing results in MasterView software.

After taking this training course, you should be able to:

  • Create an Scheduled MRM-IDA-MS/MS acquisition method in Analyst software
  • Import MS/MS libraries into MasterView software
  • Create a processing method for QTRAP MS/MS library searching in MasterView software
  • Process Scheduled MRM-IDA-MS/MS data in MasterView software
  • Review and filter results
  • Report results and export processing parameters for further processing in MultiQuant software

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Protein and Peptide Identification Workflows on SCIEX TripleTOF Systems View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

Upon completion of this training module, you should be able to:

  • Learn the SCIEX Protein/Peptide Identification Workflow
  • Understand the concepts to build an Information Dependent Acquisition (IDA) method using Analyst software
  • Evaluate IDA data using PeakView software
  • Understand the process of using ProteinPilot software to search and review data

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Qualitative Lipid Analysis : MS/MSall on TripleTOF Instruments View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

During this eLearning course, you will be:

  • Presented with current challenges in the analysis of lipids by mass spectrometry
  • Introduced to the MS/MS ALL workflow and given step-by-step instructions for its implementation
  • Introduced to data processing strategies to deal with the large data arrays generated during lipidomics analysis.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Quantitation and Targeted Identification Data Processing Using SCIEX OS Software View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course focuses on qualitative data processing using the Quantitation and Targeted Identification workflow in SCIEX OS software. This course applies to all SCIEX instruments (excluding MD instruments). 

After completing this course, you should be able to:
  • Describe the quantitation and targeted identification workflow
  • Understand the different types of library searches
  • Create a processing method
  • Create results
  • Perform qualitative data review using the results table and peak review pane
  • Export and report results

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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Quantitation and Targeted Identification Workflow with SWATH Acquisition using SCIEX X500R QTOF System View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course covers the Quantitation and targeted identification workflow with SWATH Acquisition using the SCIEX X500R QTOF system. This is an advanced course, and it is recommended that learners first complete the "Introduction to LC-MS/MS" course prior to beginning this module. Upon completion of the course, learners will be able to create an LC and MS SWATH acquisition method, acquire and process data.

The course covers the following topics:
  • What is SWATH Acquisition?
  • Creating a LC Method in SCIEX OS
  • Using the SWATH Variable Window Calculator and creating an MS Method in SCIEX OS
  • Acquiring SWATH data
  • Processing TOF-MS and TOF-MS/MS data using Analytics

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Quick maintenance how to videos for SCIEX Triple Quadrupole and QTRAP systems View Course

  • Duration: 25 minutes
  • Language: English
  • Type: Premium, How-to Videos
  • Course Level: Advanced
  • Rating:

This series of quick maintenance videos covers the SCIEX triple quadrupole and QTRAP systems. You will become familiar with performing simple maintenance tasks for your system.

General System Maintenance Videos:
  • Basic computer maintenance
Ion Source Maintenance Videos:
  • Removing and installing the ion source
  • Removing and installing the probe
  • Removing ion source covers
  • Replacing the metal turbo heater on the Turbo V ion source with external covers
  • Replacing the turbo heater on the IonDrive Turbo V ion source with external covers
  • Replacing the ceramic turbo heater on the Turbo V ion source without external covers
  • ESI probe maintenance and troubleshooting
  • APCI corona discharge needle replacement

Chapters: 9
Certificate Type: None
PACE Accreditation: No

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Quick maintenance how to videos for the ExionLC 2.0 system View Course

  • Duration: 40 minutes
  • Language: English
  • Type: Premium, How-to Videos
  • Course Level: Advanced

This series of short maintenance videos focuses on the ExionLC 2.0 system, and cover how to perform simple instrument maintenance tasks. 

ExionLC 2.0 and 2.0+ Autosampler

  • Flushing the autosampler
  • Replacing the sample needle 
  • Replacing the air needle
  • Replacing the syringe
  • Replacing the fuses

ExionLC 2.0 Binary Pump

  • Removing and installing the pump head
  • Dissembling and reassembling the pump head
  • Replacing the o-rings
  • Replacing the pump seals
  • Purging the pump
  • Replacing the check valves
  • Replacing the mixer
  • Replacing the filter cartridge
ExionLC 2.0+ Binary Pump
  • Replacing the check valves
  • Replacing the mixer
  • Replacing the stator and rotor seal
  • Replacing the filter cartridge

ExionLC 2.0 Detectors

  • Replacing the flow cell

Chapters: 18
Certificate Type: None
PACE Accreditation: No
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Quick maintenance how to videos for the Jasper HPLC system View Course

  • Duration: 5 minutes
  • Language: English
  • Type: Premium, How-to Videos
  • Course Level: Advanced
  • Rating:

This series of short maintenance videos focuses on the Jasper HPLC System, and cover how to perform simple instrument maintenance tasks. 

  • Clean Up the Leaks in the Column Oven
  • Clean the Pump Leak Tray
  • Remove Autosampler Clear Acrylic Panel (Panel F)
  • Clean the Rinsing Port & Cover and Replace Rinsing Port Cap
  • Rinse Needle and Sample loop
  • Remove Condensation
  • Replace the Fuses
  • Clean Check Valves
  • Replace the Needle Seal
  • Replace Sample Loop

Chapters: 10
Certificate Type: None
PACE Accreditation: No

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Quick maintenance how to videos for the OptiFlow Turbo V source View Course

  • Duration: 10 minutes
  • Language: English
  • Type: Premium, How-to Videos
  • Course Level: Intermediate

This series of short maintenance videos focuses on the OptiFlow Turbo V Source, and covers how to perform simple instrument maintenance tasks. 

  • Remove and reinstall the probe and electrode
  • Remove the ion source

Chapters: 2
Certificate Type: None
PACE Accreditation: No

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Quick maintenance how to videos for the SCIEX M5 MicroLC system View Course

  • Duration: 6 mins
  • Language: English
  • Type: Premium, How-to Videos
  • Course Level: Advanced
  • Rating:

This series of short maintenance videos focuses on the SCIEX M5 MicroLC System, and cover how to perform simple instrument maintenance tasks. 

  • Putting PAL Virtual Terminal into Extended User Mode
  • Replace the Valve Stator and Rotor Seal
  • Replace the Sample Loop 
  • Prime the Autosampler Wash Pumps
  • Replace The Syringe Needle
  • Replace the Syringe Barrel and Plunger
  • Measure the Flow Rate
  • Calibrate the Flow Meters 
  • Re-initialize Pressure Transducers
Chapters: 9
Certificate Type: None
PACE Accreditation: No
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Resolution Equation View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

In this course, the Resolution equation and its components will be described and how each component affects resolution.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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SCIEX Biotransformation Workflow Using the BioTrans Solution View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course covers how to acquire and process SWATH data using MetabolitePilot Software 2.0 with TOF instruments controlled either by the Analyst TF or SCIEX OS software.

After taking this course, you should be able to:
  • Describe SWATH acquisition and its workflow
  • Create SWATH acquisition methods
  • Build a batch and acquire SWATH data
  • Process, review and interpret SWATH data using MetabolitePilot for small molecule, peptide and ADC workflows

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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SCIEX NanoSpray III Ion Source View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course is designed to provide the new user a primer on how to assemble, optimize and test the NanoSpray III Ion Source. It is assumed that you have a working knowledge of mass spectrometry and Nano flow LC. Following the completion of this course, you will be able to:

  • Describe the usage of the NanoSpray III Ion Source
  • Identify the different parts of the source
  • Assemble the NanoSpray III Ion Source
  • Test that the source is functioning properly
  • Optimize methods
  • Perform basic troubleshooting processes

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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SCIEX OS - MS Tune for SCIEX Triple Quad and QTRAP MS/MS systems View Course

  • Duration: 30 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
This course covers how to use MS Tune in SCIEX OS for SCIEX Triple Quad and QTRAP MS/MS systems.  In this course, we will review the following topics:
  • Configuring devices in SCIEX OS
  • Tuning solutions
  • The Tuning Instrument data file
  • How to verify and tune your SCIEX system performance using SCIEX OS

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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SCIEX OS - Method Creation for Triple Quad and QTRAP Systems View Course

  • Duration: 30 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:
This course covers how to create LC and MS methods for SCIEX Triple Quad and QTRAP Systems using SCIEX OS. It includes the following topics:
  • Creation of an MS method
  • Advanced MS method parameters
  • Advanced features for an MS method such as ionization scheduling, multiple experiments, experiment scheduling and default settings
  • Creation of an MS method using the Scheduled MRM algorithm
  • Creation of an IDA MS method
  • Conversion of an Analyst method to a SCIEX OS MS method
  • Creation of an LC method

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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SCIEX OS - Triple Quad 和 QTRAP 系统中的方法建立(SCIEX OS - Method Creation for Triple Quad and QTRAP) View Course

  • Duration: 30 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

本课程讲授如何通过SCIEX OS 为SCIEX 三重四极杆和QTRAP 系统新建 LC 和 MS 方法。包含以下内容:

  • MS方法建立
  • 高级MS方法参数
  • MS 方法高级功能,如预设离子化、多实验、预设实验和默认参数设置 
  • 使用 Scheduled MRM 算法新建MS 方法
  • 新建 IDA MS 方法
  • 将 Analyst 方法转换为 SCIEX OS MS 方法
  • 新建LC方法

章节数: 1
证书类型: 结课
PACE 认证: 无

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SCIEX OS Security and Audit Trail View Course

  • Duration: 20 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This eLearning course will cover the security and audit trail functionality in SCIEX OS. After completing this course, you should be able to:

  • Understand how SCIEX OS works together with Windows to control and audit system access
  • Configure users and their access to SCIEX OS functionality
  • Set up audit map templates for auditing of project and workstation events
  • Review audited events using the Audit Trail workspace
  • Use the Event Log workspace to view unaudited system events 

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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SCIEX TripleTOF系统蛋白和肽段鉴别工作流程(Protein and Peptide Identification on SCIEX TripleTOF) View Course

  • Duration: 20 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

完成该培训课程后,学习者将达到以下目标:了解使用Analyst 软件构建信息依赖性采集(IDA)方法的概念;使用PeakView软件评估IDA数据;最后,了解使用ProteinPilot™软件搜索和查看数据的过程。

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SCIEX X500R QTOF系统高分辨MRM(MRMHR)工作流程(MRMHR Workflow Using SCIEX X500R QTOF) View Course

  • Duration: 25 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

本课程讲解了在SCIEX X500R QTOF系统SCIEX OS软件使用高分辨MRM工作流程的过程。建议在学习本课程之前,首先完成“LC-MS/MS 操作教程- X-系列 QTOF 系统”在线课程。完成本课程之后,学习者将能够在系统中建立优化的MS方法、提交Batch和处理数据。

课程内容章节如下:
- Guided MRM HR 化合物优化
- TOF MSMS方法编辑器
- 建立Batch 和运行scheduled MRM HR
- 使用Quantitation和 Quantitation and targeted identification工作流程处理数据

单元数量:1
证书类别:Completion
PACE认证:NO

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SCIEX 三重四极杆/QTRAP系统快速维护视频 View Course

  • Duration: 20 minutes
  • Language: Chinese
  • Type: Premium, How-to Videos
  • Course Level: Advanced
  • Rating:

本系列视频课程为SCIEX 三重四极杆/QTRAP维护相关内容,包含五个离子源相关维护操作。在视频学习之后,即可更加了解并掌握仪器系统的简单维护工作。

离子源维护视频:

- 拆卸和安装Probe
- 拆卸和安装离子源
- 拆卸和安装 IonDrive Turbo V离子源的保护罩
- 更换Turbo V离子源上的金属Turbo加热器
- 更换IonDrive Turbo V离子源上的Turbo加热器

章节:5
证书类型:无
PACE 认证:否

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SCIEX 离子源的离子化技术(Ionization Techniques for SCIEX Sources) View Course

  • Duration: 15 mins
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

本课程将向您介绍使用SCIEX离子源可以执行的不同的电离技术,以及每个离子源的重要离子源和气体参数。关于优化OptiFlow Pro Ion source源参数的指南,可以在课程页面顶部的参考资料选项卡中访问。

本课程涵盖以下主题:

  • ESI,、APCI和APPI电离
  • Turbo V、IonDrive Turbo V、PhotoSpray、DuoSpray OptiFlow Turbo V源,以及OptiFlow Pro离子源
  • 重要的离子源参数

章节: 1
证书类别: 结课
PACE 认证: No

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SWATH Acquisition Performance Kit for MicroLC View Course

  • Duration: 30 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate

This eLearning course will show you how to assess the performance of your TripleTOF system for microLC using the SWATH Acquisition Performance Kit. For data processing, you will need to use PeakView Software, SWATH Acquisition MicroApp, SWATH Replicate Analysis template and ProteinPilot Software. 

After completing this course, you should be able to:
  • Perform a direct infusion test using PepCalMix
  • Perform a system performance test using K562 Extract and PepCalMix
  • Perform autocalibration using PepCalMix
  • Check the performance of SWATH acquisition
  • Assess the protein identification performance

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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SWATH Acquisition Performance Kit for NanoLC View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This eLearning course will show you how to assess the performance of your TripleTOF system for nanoLC using the SWATH Acquisition Performance Kit. For data processing, you will need to use PeakView Software, SWATH Acquisition MicroApp, SWATH Replicate Analysis template and ProteinPilot Software.  After completing this course, you should be able to:

  • Perform a direct infusion test using PepCalMix
  • Perform a system performance test using K562 Extract and PepCalMix
  • Perform autocalibration using PepCalMix
  • Check the performance of SWATH acquisition
  • Assess the protein identification performance

Chapters: 1
Certificate Type: Completion
PACE Accreditation: None

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Sample Preparation Strategies for Biologics Characterization View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course covers common sample preparation strategies for biologics characterization. 

After taking this training course, you should be able to:

  • Understand common sample preparation techniques for intact MS analysis of therapeutic biologics
  • Identify common MS parameters for analysis of biologics in both native and denatured forms
  • Identify common protocols for biologics protein digestion, including single and combination protease use
  • Understand the protein digest cleanup method of reversed phase solid phase extraction to remove salts and other contaminants prior to MS analysis.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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System Integration Test and Autocalibration with Beta Galactosidase Digest Standard on a TripleTOF System View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

The objective of this training module is to instruct in the creation of a BetaGal standard peptide digest method. This method will aid you in testing the integration and performance of your TripleTOF system with its Eksigent NanoLC front end. Additionally you will use this BetaGal method to perform automated calibrations of your TripleTOF system.

After taking this course you will be able to:
  • Create and implement the LC-MS system integration test using the BetaGal standard, and monitor your LC-MS performance
  • Create and modify the calibration reference table for BetaGal
  • Submit an autocalibration method to update the instrument default calibration
  • Use PeakView software to perform post-acquisition data processing

The Bgal_XIC excel file mentioned in the course is located in the resource section to the left of this description.

Chapters: 1
Certificate Type: None
PACE Accreditation: No

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Triple Quad 6500 and QTRAP 6500 System Optimization View Course

  • Duration: 20 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This course takes you through the key strategies for optimizing sensitivity on your Triple Quad 6500 and QTRAP 6500. We focus on the source setting differences between this system and our previous generation systems.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Triple Quad and QTRAP System Integration and Performance Test with Beta Galactosidase Digest Standard View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

The objective of this training module is to instruct in the creation of a Beta-gal standard peptide digest method. This method will aid you in testing the integration and performance of your Triple Quad or QTRAP system with its Eksigent NanoLC 400 front end. After taking this course you will be able to create and implement  the  LC/MS system integration test using the beta-gal standard, and will be able to monitor your LCMS performance in MRM mode.

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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TripleTOF 6600/6600+ 系统关于蛋白质组学应用的问题解决(Troubleshooting TripleTOF 6600/6600+ System Performance for Proteomics) View Course

  • Duration: 30 minutes
  • Language: Chinese
  • Type: Premium, eLearning Course
  • Course Level: Advanced

本课程旨在帮助您解决TripleTOF 6600和6600+系统的蛋白质组学应用系统性能问题。

完成本课程后,将能够

描述 TripleTOF 6600/6600+ 系统在蛋白质组学应用中可能出现的常见问题

了解如何使用调谐液和 SWATH 采集性能套件来诊断问题

鉴定系统的 Q0 或 IQ0 前端污染

识别由 LC 组件引起的问题

章节: 1
证书别: 结课
PACE 认证: No

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Troubleshooting TripleTOF 6600/6600+ System Performance for Proteomics Applications View Course

  • Duration: 30 mins
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Advanced
  • Rating:

This course is designed to enable you to troubleshoot system performance issues for the TripleTOF 6600 and 6600+ System with proteomics applications. 

After completing this course, you should be able to:

  • Describe common issues that occur for proteomics applications with the TripleTOF 6600 and 6600+ System
  • Understand how to use the Tuning Solution and SWATH® Acquisition Performance Kit to diagnose issues
  • Identify Q0 or IQ0 front end contamination of your system
  • Recognize issues that could be caused by the LC components
Chapters: 1
Certificate Type: Completion
PACE Accreditation: None
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Using the PeakView Bio Tool Kit for Analyzing Proteomics Data View Course

  • Duration: 30 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

After taking this training course, you should be able to:

  • Understand how to manually determine possible peptide sequences from a proteomics IDA datafile using the ‘Manual Sequence’ function, and link an experimental spectrum to a theoretical peptide sequence;
  • Take peptide data and process with the “LC/MS Peptide reconstruct” function to deconvolve spectral peaks and produce a peptide map;
  • Utilize the ‘Digest Protein’ function to obtain all theoretical peptide sequences from a protein of interest, and how to link this back to peptide mapping data;
  • Use the ‘Manual Reconstruct’ function for both peptide and protein data to identify the MW and potential charge states of a target;
  • Obtain a deconvolved, zero charge mass graph for an intact protein target using the ‘Protein Reconstruct’ function

Chapters: 1
Certificate Type: Completion
PACE Accreditation: No

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Working With MultiQuant MD Software View Course

  • Duration: 25 minutes
  • Language: English
  • Type: Premium, eLearning Course
  • Course Level: Intermediate
  • Rating:

This course introduces the topic of quantitation using the MultiQuant MD software. Upon completion of this course, learners will be able to process data, review the results, and export data. 

The course covers the following topics:
  • Create a processing method and set default method parameters
  • Process data and review results
  • Export data and create reports
  • Review data using the Audit Trail Viewer

    Chapters: 1
    Certificate Type: Completion
    PACE Accreditation: No

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    Working With MultiQuant Software View Course

    • Duration: 60 minutes
    • Language: English
    • Type: Premium, eLearning Course
    • Course Level: Intermediate
    • Rating:

    This course is designed for learners to become familiar with MultiQuant software.

    Upon successfully completing this learning module, you will be able to:

    • Select a MultiQuant software integration algorithm
    • Use the MultiQuant Wizard to process data and generate a results table
    • Review peaks and change integration parameters
    • Generate calibration curves, plot metrics, and analyze statistics
    • Create reports using the Reporter Tool.

    Chapters: 1
    Certificate Type: Completion
    PACE Accreditation: No

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    Working with Diverter And Manual Injector Valves View Course

    • Duration: 20 minutes
    • Language: English
    • Type: Premium, eLearning Course
    • Course Level: Intermediate
    • Rating:

    The course introduces the subject of how and why you would use diverter valves and manual injector valves. Upon completion of the course, learners will be able to successfully use integrated and external valves as a diverter valve or manual injector valve for sample analysis.

    The course covers the following topics:
    • What are diverter valves?
    • How to configure a hardware profile for built-in and external diverter valves
    • How to plumb an integrated valve as a diverter valve or manual injector valve
    • Creating an acquisition method using an integrated or external valve
    • Using Delay Time in a method

    Chapters: 1
    Certificate Type: Completion
    PACE Accreditation: No

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    使用 SCIEX OS 软件进行非靶向筛查数据处理(Non-targeted Screening Data Processing Using SCIEX OS) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本在线学习课程侧重于使用SCIEX OS软件的Analytics工作区中的非靶向筛查工作流进行数据处理。适用于所有SCIEX仪器(MD仪器除外)。

    完成本课程后,将能够:
    - 描述非靶向工作流程
    - 了解如何在数据查看中使用数据库检索和公式查找
    - 新建一个处理方法
    - 新建结果
    - 进行数据查看
    - 导出数据和新建报告

    章节: 1
    证书类别: 结课
    PACE 认证: 无

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    使用 SCIEX TripleTOF 质谱仪的生物药表征分析工作流程(Biologics Workflows Using SCIEX TripleTOF) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    在本课程中,我们将带您了解使用SCIEX TripleTOF系统进行完整的蛋白质分子量、肽标测和定量、宿主细胞蛋白检测的工作流程,还将讲到把CESI作为一种额外的分离策略。

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    使用 SCIEX X500B QTOF 系统 和 BioPharmaView软件进行完整生物制剂分析(Intact Biologics Analysis) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    在本课程中,我们将介绍SCIEX X500B QTOF系统和BioPharmaView软件,并演示完整生物制剂分析的工作流程。
    完成本课程后,将能够:

    - 了解完整生物分析的基础知识
    - 熟悉硬件和软件特性
    - 在SCIEX OS中创建LC和 MS 采集方法
    - 提交Batch
    - 使用 BioPharmaView 软件处理完成生物制剂数据

    章节: 1
    证书类别: 结课
    PACE 认证: No

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    使用 SCIEX X500B QTOF 系统和 BioPharmaView 软件进行肽图分析(Peptide Mapping Analysis Using SCIEX X500B QTOF and BioPharmaView) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本课程介绍了SCIEX X500B QTOF系统和BioPharmaView软件,并演示了肽图分析的工作流程。

    完成本课程后,将能够

    - 理解肽图分析的基础
    - 在SCIEX OS中新建LC和MS方法
    - 在SCIEX OS中提交Batch
    - 使用 BioPharmaView 软件处理肽图数据

    章节: 1
    证书类别: 结课
    PACE 认证: No

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    使用 SCIEX X500R QTOF 系统进行 SWATH Acquisition 的定量和靶向鉴别工作流程(Quantitation and Targeted Identification with SWATH Acquisition using SCIEX X500R) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本课程涵盖了使用SCIEX X500R QTOF系统进行SWATH采集的定量和靶向鉴定工作流程。这是一个高级课程,建议学习者在开始这个模块之前先完成“LC-MS/MS入门”课程。完成该课程后,学员将能够创建LC和MS SWATH采集方法,采集和处理数据。

    本课程涵盖以下内容:

    • 描述 SWATH Acquisition
    • 在SCIEX OS中创建 LC 和 MS 方法
    • 使用 SWATH 变量窗口计算器并创建MS方法
    • 采集 SWATH 数据
    • 处理 SWATH TOF-一级和二级质谱数据

    章节: 1
    证书类别: 结课
    PACE 认证: No

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    使用 SCIEX X500R QTOF 进行非靶向筛查 (Non-targeted Screening Workflow Using SCIEX X500R QTOF System) View Course

    • Duration: 30 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本课程介绍了使用SCIEX X500R QTOF系统与SCIEX OS的非靶向筛选工作流程。这是一门高级工作流程课程,强烈建议您在学习这门课程之前完成X500R QTOF系统在线学习系列LC-MS/MS操作教程。完成课程后,学习者将能够在筛查工作流程中创建LC和MS方法、采集数据,并使用分析处理所获得的数据。

    课程包含以下内容:
    • 新建LC 和MS 方法
    • 新建批次和采集数据
    • 在Analytics中使用Non-targeted screening workflow处理数据
    章节: 1
    证书类别: 结课
    PACE 认证: No

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    使用MasterView软件处理 QTRAP MS/MS数据用于鉴别化合物(Processing of QTRAP MS/MS Data in MasterView) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本课程介绍如何使用MasterView软件处理QTRAP数据。完成课程后,学习者将能够学会如何创建采集方法和数据处理方法,以及在MasterView软件中查看其处理结果。

    课程将涵盖以下内容:
    • 在Analyst软件中创建一个Scheduled MRM-IDA -MS/MS 采集方法
    • 将MS/MS库导入MasterView软件
    • 在MasterView软件中创建QTRAP MS/MS数据库搜索方法
    • 在MasterView软件中处理Scheduled MRM-IDA -MS/MS数据
    • 查看和筛选结果
    • 生成报告结果和导出能用于MultiQuant软件的参数

    章节数: 1
    证书类型: Completion
    PACE 认证: 无

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    如何充分发挥Scheduled MRM演算法的作用(Getting the Most Out of the Scheduled MRM) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Advanced

    本课程主要Scheduled MRM演算法理论,以及如何使用该算法创建方法。课程中还介绍了该算法在Analyst软件1.7版中的增强功能。

    学习本课程将能够获得以下技能:

    • 能够解释Scheduled MRM 演算法的原理
    • 能使用基础和高级参数新建一个方法
    • 可以描述在新建方法时应遵循的准则
    • 会使用 sMRM 计算器插件优化方法

    证书类型:完成
    PACE认证:No

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    完整多肽定量样品前处理技术 View Course

    • Duration: 30 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate
    • Rating:

    本课程的学习约需用时30分钟。

    课程内容:
    -了解蛋白沉淀技术的基本原理
    -了解样品处理方法中的同位素内标多肽及其使用
    -分辨不同类型的固相萃取方法,选择合适的条件富集特定多肽
    -学会计算基质效应及提取回收率
    -了解特定多肽的亲和富集策略

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    定性脂质分析:在 TripleTOF 仪器上进行的 MSMSALL 分析(Qualitative Lipid Analysis : MS/MSall on TripleTOF) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本在线学习课程将介绍质谱分析脂质的当前挑战,介绍MS/MS ALL工作流程并逐步说明其如何实现。还将介绍脂质组学分析过程中产生的大数据的数据处理策略。

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    小分子分析样品处理流程 View Course

    • Duration: 30minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate
    • Rating:

    本培训课程提供了目前小分子领域最常用的前处理方法相关流程。

    主要涵盖以下三个领域:

    • 多农残检测和筛查
    • 非靶向代谢组学
    • 非目标脂质组学

    该课程内容由SCIEX应用专家提供,力求提供最实用有效的小分子前处理方法。

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    应用技巧培训:如何实现最佳灵敏度 (Application Tips for Optimal Sensitivity) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate
    • Rating:

    本自助在线培训为三重四极杆和QTRAP系统操作者提供了提高系统灵敏度的经验建议。

    课程级别:基础

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    方法开发-流动相篇 (Method Development - Mobile Phase) View Course

    • Duration: 20minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate
    • Rating:

    本课程介绍了液相方法开发中如何使用反相流动相。课程内容包括液相方法开发中的溶剂选择、流动相pH和缓冲溶液、等度洗脱与梯度洗脱的比较,还讲到了色谱柱的温度。

    单元数: 1

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    方法开发-色谱柱的选择性篇 (Method Development - Column Selectivity) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本课程的主要内容是液相方法开发中色谱柱的选择性。学习本课之后,学员可以达到:
    -理解影响色谱柱选择性的关键因素
    -对C18、C8、C4、苯基和PFP反相色谱柱有一定了解
    -理解极性化合物在正相色谱柱、带极性官能团的色谱柱和HILIC柱上的选择性

    单元数: 1

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    运用SCIEX OS软件进行定量与靶向筛查数据处理(Quantitation and Targeted Identification Data Processing Using SCIEX OS) View Course

    • Duration: 20 minutes
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本课程主要介绍使用SCIEX OS软件的定量和目标筛查工作流程进行定性数据处理。本培训适用于所有SCIEX仪器(包含MD仪器)。

    在完成本课程之后,将能够:

    • 描述定量与靶向筛查工作流程
    • 理解不同类型的数据库搜索
    • 新建处理方法
    • 新建结果
    • 使用结果表格和Peak Review窗格查看定性数据
    • 导出和生成报告

    单元数:1
    证书类型:Completion
    PACE 认证:无

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    适用于 MicroLC 的 SWATH 采集性能套件(SWATH Acquisition Performance Kit for MicroLC) View Course

    • Duration: 30 mins
    • Language: Chinese
    • Type: Premium, eLearning Course
    • Course Level: Intermediate

    本电子学习课程将向您展示如何使用SWATH采集性能工具包评估microLC的TripleTOF系统的性能。对于数据处理,您将需要使用PeakView软件、SWATH采集MicroApp、SWATH Replicate Analysis模板和ProteinPilot软件。

    完成本课程后,你将能够:

    • 使用 PepCalMix 进行直接注入进样测试
    • 使用 K562 提取物和 PepCalMix 进行系统性能测试
    • 使用 PepCalMix 进行自动校准
    • 检查 SWATH 采集的性能
    • 评估蛋白鉴定性能

    章节: 1
    证书类别: 结课
    PACE 认证: No

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    适用于 NanoLC 的 SWATH 采集性能套件(SWATH Acquisition Performance Kit for NanoLC) View Course

    • Duration: 30 minutes
    • Language: Chinese
    • Type: Premium
    • Course Level: Intermediate

    本电子学习课程将向您展示如何使用SWATH采集性能工具包评估NanoLC的TripleTOF系统的性能。对于数据处理,您将需要使用PeakView软件、SWATH采集MicroApp、SWATH Replicate Analysis模板和ProteinPilot软件。

    完成本课程后,你将能够:
    • 使用 PepCalMix 进行直接注入进样测试
    • 使用 K562 提取物和 PepCalMix 进行系统性能测试
    • 使用 PepCalMix 进行自动校准
    • 检查 SWATH 采集的性能
    • 评估蛋白鉴定性能

    章节: 1
    证书类别: 结课
    PACE 认证: No

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