Overview
The analysis of mRNA is complex due to its fragile nature, heterogeneity and length.
Discover workflows that let you break through the analytical boundaries of in vitro transcribed (IVT) products and provide quality results to confidently address the challenges of mRNA, circRNA and saRNA development.
Determine CQAs, integrity, purity and secondary structures with intuitive solutions that provide meaningful answers to keep your process in progress.
Workflow
mRNA integrity and purity
mRNA product quality is directly linked to integrity and purity.
Enable control of your RNA products. Assess the purity of full-sized mRNA molecules with the highest quality and resolution, and create pathways for analyzing and understanding these fragile molecules more easily and reliably.
Kit-based solutions let you save time so you can focus on your research and help determine the future of medicine.
mRNA integrity and purity
Solution
- High-quality separation
- Accelerated method development and mRNA sample analysis
Featured resources
Take control of impurities and artifacts in your mRNA-lipid nanoparticle (LNP) products. Achieve high-quality, robust assessment of the integrity and size of mRNA molecules encapsulated in LNPs.
Learn from scientist Jérémie Parot (SINTEF) how to break through the boundaries of mRNA analysis.
Discover how to save time by streamlining your CE data management with the Empower CDS.
mRNA integrity and purity
Solution
Workflow
CircRNA
The use of next-generation RNA, such as circular RNA (circRNA)—a type of RNA that is not dependent on CQAs such as 3' poly-A tails or 5' capping—is growing.
Pursue improved RNA-based drugs with the ability to confidently separate circRNA from linear precursors and assess a continuous loop of RNA using high-quality data.
CircRNA
Solution
- High-quality separation
- Accelerated method development and mRNA sample analysis
CircRNA
Solution
Workflow
5’ capping
Imagine taking control of your mRNA stability and translation efficiency by better understanding your 5’ CQAs.
Characterize and quantify capped vs. uncapped mRNA species and dig deeper into distinguishing different capping intermediates with high-quality data generated using solutions based on capillary electrophoresis (CE) and accurate mass spectrometry.
5’ capping
Solution
- Identification and quantitation of 5'-end capping intermediates
- Robust, analytical flow setup
5’ capping
Solution
- Identification and highly sensitive quantitation of capping intermediates
- Meeting flexibility needs to perform a range of additional workflows
5’ capping
Solution
- Differentiation of capped from non-capped 5' ends
- Assay transfer to quality control (QC)
Workflow
3’ poly-A tail
Understanding product stability lays the foundation for improved drugs.
Lead the way by characterizing your 3’-end CQAs, whether you are using template-encoded tails, enzymatic addition or a combination of these approaches.
Give your team the power to determine the lengths of the adenosine tails and their distribution profiles in mature mRNA products with solutions based on CE and accurate mass systems.
3’ poly-A tail
Solution
- Molecular weight determination of 3'-end poly-A tails
- Robust, analytical flow setup
3’ poly-A tail
Solution
- Molecular weight determination of 3'-end poly-A tails
- Meeting flexibility needs to perform a range of additional workflows
3’ poly-A tail
Solution
Workflow
Secondary structure
RNA structures can affect your LNP formulations and the stability and efficacy of a final product. Gain greater control of structural prediction for your oligonucleotides of interest. Leverage solutions that gather empirical data without nucleotide or structural bias to enable better insights into RNA backbone flexibility based on base-paired and unconstrained residues.
Secondary structure
Solution
All resources
Break through the barriers to developing better LNP-based drugs and make better use of your time with streamlined CE and MS workflows for mRNA-LNPs.
Self-amplifying RNA (saRNA) can enable lower dosing and expand the range of potential indications. However, srRNAs are often much larger in size (>9,000 bases). Learn how to assess these large molecules with high-resolution assays.
Take control of impurities and artifacts in your mRNA-lipid nanoparticle (LNP) products. Achieve high-quality, robust assessment of the integrity and size of mRNA molecules encapsulated in LNPs.
Learn from scientist Jérémie Parot (SINTEF) how to break through the boundaries of mRNA analysis.
Discover how to save time by streamlining your CE data management with the Empower CDS.
Set your own schedule for mRNA analyses by streamlining your CE data management with the Empower CDS.
Create pathways for effective method development and learn how to achieve the highest quality data for RNA products with sizes ranging from 50–9,000 nucleotides and beyond.
Pave the way to better characterization of circRNA generated from linear precursors and take your RNA analysis to the next level.