Targeted profiling using the SCIEX Triple Quad™ 7500 LC-MS/MS System - QTRAP® Ready
Paul Norris, Santosh Kapil Kumar Gorti, Mackenzie Pearson
SCIEX, USA
In biological samples, reliable detection and quantification of lipid mediators can be challenging because these molecules are locally acting, short-lived, and produced in small quantities (often in the femtogram to nanogram range). Detecting lipid mediators is also challenging because of the complexity and diversity of different tissue matrices which can suppress ionization and negatively affect lower limits of detection and quantification when using ESI-MS/MS. Various method development techniques can be employed to help improve the recovery and detection of analytes, such as rigorous extraction protocols or employing microflow chromatography, but these can be laborious, costly, and in some cases too time consuming to perform and maintain routinely.
LC-MS/MS analysis for the targeted quantification of lipid mediators has emerged as a powerful workflow to characterize and profile lipid mediators.1 Still, innovations that can unlock new levels of sensitivity are needed to advance the biological understanding and pathway interpretation of lipid mediator production in a range of physiological processes for personalized and precision medicine research. With technological improvements on the SCIEX Triple Quad 7500 System, the lower limits of quantification and detection have been improved compared to previously attained data from the QTRAP 6500+ LC-MS/MS System.1
In this report, an expanded assay panel with LLOQs down to the lower femtogram range is described. Figure 1 compares the sensitivity levels for the same sample analyzed on the QTRAP 6500+ System and on the SCIEX 7500 System. Highlighting compounds detected near their LLODs and LLOQs, and compounds simply undetected on the QTRAP 6500+ System, significant gains in sensitivity and signal to noise were observed on the SCIEX 7500 System. Several sample matrices, including NIST 1950 Metabolites in Human Frozen Plasma as well as mouse liver, brain and human serum, were analyzed to further demonstrate sensitivity improvements.