Ultrasensitive bioanalytical quantification with minimal method development time
What do you get with the 7500 system is combined with Micro LC? An analytical platform for routine, highly sensitive bioanalytical sample analysis! The MMAE LLOQ in rat plasma was achieved using a generic, unoptimized protein precipitation sample preparation, and a generic HPLC gradient with standard stationary phase and mobile phases. The ability to routinely detect ultra-low analyte levels, with minimal method development time, will allow bioanalytical groups to deliver high quality data with rapid turnaround times.
Sensitivity gains for the evolution of routine bioanalysis
The rapid advancement of the drug pipeline demands sensitivity to allow a more complete pharmacokinetic
characterization of low-dose drug candidates at low detection limits. The SCIEX 7500 system will enable
pharmaceutical researchers to continue to explore lower dosage, higher efficacy compounds and to improve
the efficiency of routine bioanalysis. Learn more in this technical note.
Pharmacologically relevant measurement of high potency drug candidates
with improved sensitivity in In-vitro transporter assays
In drug discovery, being able to perform pharmacologically relevant measurements of drug candidates and
evaluate the transporter at early-stage development is crucial when selecting drug safety profiles. The
SCIEX 7500 system offers you enhanced sensitivity to reduce compound concentrations, so it’s more
biologically relevant. Download this technical note to see how you can get higher-quality data.
High sensitivity MRM workflow for signature peptide quantification
Quantification of peptide/protein therapeutics in biological matrices is crucial for therapeutic
development. Download this technical note, which demonstrates a robust method that uses the SCIEX 7500
system to quantify a series of surrogate peptides in rat plasma at ultra-low LLOQs, ranging from 5 to 39
Antisense oligonucleotides in rat plasma using trap-and-elute with microflow LC
Ultra-low LLOQs for ASOs in rat plasma were achieved using a trap-and-elute microflow method coupled to the SCIEX 7500 System, allowing for pharmacokinetic studies with limited sample availability that require quantification at trace levels. 20x improvement in sensitivity was achieved compared with previous analyses at analytical flow rates. A 3x improvement in sensitivity was observed compared with using the same trap-and-elute microflow method with the QTRAP 6500+ System. The 10–50x lower solvent consumption of the trap-and-elute microflow method can result in significant savings in expensive, high-purity modifiers such as hexafluoroisopropanol. The lower solvent consumption of the trap-and-elute microflow method also promotes robustness by reducing the level of ion-pairing reagents introduced into the mass spectrometer.
Development of an ultra-sensitive assay for anti-sense oligonucleotide quantification
An ultra-sensitive LC-MRM method for quantifying a highly modified ASO was demonstrated with a 4-fold enhancement in S/N compared to previous instrument generations. Previously unseen sensitivity for ASO quantification with low LLOQ at in matrix was achieved, combining exceptional accuracy and reproducibility with an LDR of 4.5 orders of magnitude. High throughput needs were addressed with a 6-minute method run time. This highly specific LC-MS assay allows for simultaneous quantification of multiple closely related species which cannot be achieved with orthogonal assays.
Characterization of liposomes as a drug delivery vehicle
Unparalleled sensitivity and dynamic range for broad profiling of liposomes and lipid nanoparticles was achieved with the SCIEX 7500 system. This MRM assay was proven to be comprehensive and customizable, providing a targeted and flexible solution to lipid profiling. The combination of advanced hardware and streamlined software in SCIEX OS presents a cutting-edge solution for targeted quantification in a high-throughput workflow.
Ultra-sensitive LC-MRM analysis for trastuzumab emtansine quantification in rat plasma
A highly sensitive immunoaffinity-LC-MRM workflow for quantifying trastuzumab emtansine in rat plasma is presented here. By using the SCIEX 7500 System coupled with analytical flow HPLC, trastuzumab-emtansine was solidly quantified down to the single digit ng/mL level. Good reproducibility, accuracy, and wide linear dynamic range (4.5 orders of magnitude) were achieved simultaneously.
Enhanced sensitivity for cell culture media analysis (CCMA)
An ultra-sensitive scheduled MRM-based CCMA method is presented here. An average of 4-fold improvement in S/N was observed for CCM compounds across various classes. For low level target analytes, detection and quantification limits were substantially improved on the SCIEX 7500 System compared to previous generations of mass spectrometers.
Ultra-sensitive quantification of intact insulin lispro in rat plasma
An ultra-sensitive MRM based workflow using the SCIEX 7500 System was established for intact insulin lispro quantification in rat plasma. Insulin lispro was quantified at sub-ng/mL level in rat plasma with outstanding reproducibility, accuracy, and linearity. Combining the OptiFlow Pro Ion Source with E-Lens Technology and D-Jet Ion Guide provided a 4-fold improvement in S/N over the previous generations of mass spectrometers.
Enhancing the sensitivity of peptide quantification for the targeted host cell proteins analysis
A targeted HCP quantification workflow using the SCIEX 7500 System has been developed to demonstrate the high sensitivity, high analysis throughput, robustness, and multiplexing capability of the platform. An average 4-fold S/N improvement was observed over previous generations of mass spectrometers. Superior sensitivity was also achieved, with 2/3 of target proteins displaying LLOQs in the 0.02-1 ppm range, and the rest of the proteins in the range of 1-4.54 ppm. A total of 48 proteins were quantified in an 8 min LC-MS/MS run with high confidence (4 transitions per protein).
How to ensure compliance for your biotherapeutics from development throughout QC
SCIEX OS Software is a unique solution for integrating data acquisition (SCIEX X500 Series QTOF Systems and the SCIEX 7500 System) and data processing of all SCIEX file formats in a compliant-ready manner. A customizable project-based audit trail system allows the running of compliant and non-compliant projects on the same workstation for full flexibility. The audit trail function is compatible with any qualitative and quantitative workflow in SCIEX OS Software, allowing for processing in a single compliant environment. All project or workstation-based events are stored in a central audit trail that allows the visualization of any change for full traceability and easy navigation through the records. Audit trails can be exported and saved as CSV files for additional flexibility, and are automatically archived when the number of records exceeds a given number to maintain ease of navigation.
A robust microflow LC-MS/MS system for biopharmaceutical analysis
Reliable integration of the ACQUITY UPLC M-Class system with the SCIEX OS software is demonstrated here. 1000 injections were performed consecutively, plus another 100 for the calibration curve. LLOQ at single digit ng/mL was achieved for a signature peptide of the antibody trastuzumab emtansine, with an LDR of 3.3 orders of magnitude. Trap-and-elute capability allows for large sample volume analysis without increasing method run time to increase sample throughput. Easily switch between analytical and microflow setup without need for electrode position optimization, using the OptiFlow Pro ion source with E-Lens probe. SCIEX OS software increases productivity by providing a user-friendly interface and one single and compliance-ready platform for data acquisition, processing and management.
Improved LC-MRM quantification sensitivity for cyclic peptides from the
natriuetic peptide family
Quantifying trace-level cyclic peptides in biological matrices is extremely challenging. The SCIEX 7500
system can offer outstanding reproducibility, precision, accuracy and linearity to ease these challenges.
Download this ultra-sensitive, MRM-based cyclic peptide quantification workflow.
Highly sensitive quantification of proteins from the SARS-CoV-2 antigen in nasopharyngeal swab samples
An existing targeted peptide quantification assay has been refined and developed to investigate the potential use of nasopharyngeal swabs in a saline for SARS-CoV-2 coronavirus protein quantification. A QconCAT stable isotope labeled standard for increased robustness was used for swab sampling efficiency, inter-laboratory comparison, and analysis time reduction for use in high-throughput environments. The sensitivity gains attained by moving the assay onto the SCIEX 7500 System, and matrix effect reduction by changing swab storage medium, could be further increased by the use of sample enrichment and clean-up strategies, such as SISCAPA9 or SPE, or by moving to a more MS-friendly swab storage matrix.
Higher sensitivity analysis of a large panel of lipid mediators
A high-throughput, targeted, quantitative LC-MS/MS assay with low femtogram sensitivity was designed to monitor lipid mediators. Fast polarity switching, plus individualized and optimized conditions, allows for a wide diversity of lipid mediator species in both positive and negative ion mode to be analyzed within one 20-minute MRM assay. The sMRM library also includes endocannabinoids, other ethanolamide lipid mediators, as well as additional negative mode lipid mediators. Sensitivity and LLOQ gains were impressive for this application.
Analysis of genotoxic nitrosamines in losartan using the SCIEX Triple Quad™ 7500 LC-MS/MS ꟷ QTRAP® Ready System
This application note demonstrates good chromatographic separation of six analyzed nitrosamine compounds from the losartan API, and good linearity is exhibited over the concentration range monitored. The lower limits of quantification for each compound translates to daily intake limits significantly lower than those currently specified by the FDA and EMA. This ensures a suitable method for lot release in the event of future increased restrictions around these important contaminants.
Large scale targeted metabolomics assay for quantitative plasma profiling
This large-scale polarity switching method using Scheduled MRM Algorithm has been developed to quantify ~336 serum metabolites in plasma, and can be used for large scale targeted metabolomics studies. The sMRM Pro Builder tool, was developed to streamline optimization of the sMRM assay and provide insights on MRM assay data quality. Time scheduling of MRMs provided significant improvements over unscheduled methods for this large of a number of MRM transitions, with 95% of positive mode data and 80% of negative mode data exhibiting peak area CVs at <15%.
Significant sensitivity increases provide 30% more polar metabolites quantified in plasma
A targeted MRM assay with extracted NIST SRM 1950 plasma was performed to compare the QTRAP 6500+ System and the SCIEX 7500 System performance. Detection and quantification of ~30% more metabolites in plasma were realized. Specifically, there was a 25% increase in positive mode, and a 41% increase in negative mode. Find out through this technical note how the 7500 System enables significant S/N improvements for a large range of metabolites.
Achieve broad lipid quantitation using a high-throughput targeted
The complexity of the lipidome is very high, with a huge diversity in lipid molecular species, making
comprehensive quantitative profiling challenging. Download this technical note to find out about a
targeted MRM assay approach that enables quantification of a broad array of different lipids at the
molecular species level using the SCIEX 7500 system.
Reproducible targeted peptide profiling using highly multiplexed MRM
Quantitative robustness key to ensuring both large and small biological changes are accurately measured
across large sample cohorts. The SCIEX 7500 system offers you high sensitivity as well as a wide linear
dynamic range for large-scale, targeted peptide quantification. Download this technical note to learn
Determination of pyrethroids and macrocyclic lactone insecticides in spices and tea using the SCIEX 7500 System
The SCIEX 7500 system provides impressive levels of sensitivity, robustness, and accuracy for trace quantification of insecticides in food matrices. In this study, excellent sensitivity has been demonstrated with LLOQ values down to 0.02ng/mL. Quantification of pyrethroids and macrocyclic lactones in both green tea and spices has also been shown. By using a higher sensitivity LC-MS platform, injection volumes were reduced, lowering ion suppression without impacting overall assay sensitivity. The reduced amount of matrix injected for large studies help to improve total system uptime.
Highly sensitive pesticide analysis in baby food
Detecting and quantifying pesticide residues in food remains a moving target as food regulations drive
the pursuit of lower limits of quantification. This technical note demonstrates the high sensitivity of
the SCIEX 7500 system in quantifying 209 pesticides, easily achieving sub-ppb detection limits with a very
small injection volume.
Precise testing of pesticides in food using the SCIEX 7500 system
The global scale of pesticide use and its impact on food safety and authenticity regulations have led to
the need for sensitivity analysis that can achieve trace levels. Download this technical note to learn
more about how the SCIEX 7500 system offers sensitivity, robustness and accuracy with 700 compounds
achieving limits as low as 0.2 ng/mL.
The Direct aqueous analysis of pesticides and PPCPs in drinking and
bottled water at parts per trillion levels
Water can be a challenging matrix, especially if it is sourced from a
river, lake or reservoir before entering the water treatment process. Laboratories that perform testing
for water companies must
have highly sensitive instrumentation to handle these matrix challenges and meet regulatory requirements.
This technical note demonstrates the sensitivity levels of the SCIEX 7500 system during water testing.
Low level quantification of PFAS in water samples with high sensitivity and precision
The increased sensitivity of the SCIEX 7500 system allows a new level of performance for the analysis of PFAS compounds. Lower-level compounds and important lower-level isomers can be detected and quantified, with improved S/N leading to lower limits of detection. Unexpected compounds that are detected can be quantified with higher certainty. Intuitive and friendly SCIEX OS software handles all data acquisition and processing, streamlining the entire workflow.
Reducing the effects of system contamination in PFAS analysis
We present a single 10-minute LC-MS/MS acquisition method for reproducible calculated PFAS concentrations. This was enabled by high-quality separation on the ExionLC 2.0 system. Accurate measurement of PFAS amounts in environmental samples is crucial in the management of the PFAS background noise from the analytical instrumentation. Optional wash system and additional delay columns (minimizing carryover) reduced false positive rates and the need for repeat extractions and re-injections. FEP tubing replacement and delay column installation also contributed to minimizing background contamination to allow sensitive MDLs for the entire suite of 52 PFAS compounds.
Confident quantification of per- and polyfluoroalkyl substances within water intended for human consumption
An LC-MRM method using a smaller volume direct injection approach has proven to be ideal for meeting the current recommended limits set by the European authorities for drinking water. Several analytical challenges have been well documented for PFAS analysis in the past, such as blank contamination, compound stability, and the need to inject large volumes of samples. This method has successfully addressed these problems, ensuring that it can be much more easily implemented compared with previous methods. Specialized equipment or extensive modifications to the LC system were not needed.
High sensitivity drug analysis using dried blood spots
A highly sensitive workflow for the detection of 24 drugs and metabolites in DBS is presented using the SCIEX 7500 system. The broad applicability of the optimized sample extraction procedure in combination with the sensitivity of the system enabled accurate quantification at low levels of a panel of 24 chemically diverse drugs and metabolites.
Highly sensitive quantification of 12 drugs of abuse in wastewater using a simple dilution approach
Simplified sample preparation coupled with a direct injection method on the SCIEX 7500 system enabled accurate identification and sensitive quantification of drugs of abuse in wastewater. The developed workflow enabled confident identification and accurate quantification of drugs in real wastewater samples, demonstrating the applicability of the method for drug market surveillance comparable to traditional epidemiological approaches.
Reaching new sensitivity levels for the detection of fentanyl analogs and highly potent novel synthetic opioids in blood
The combination of scheduled MRM and SCIEX OS software enables sensitive detection of 32 NSOs extracted from authentic forensic blood samples. LDR of 10 to 100,000pg/mL, LLOD at single digit pg/mL, and several other new advantages on postmortem case samples (that would normally go undetected) provides a much clearer picture for forensic scientists determining causes of death.
High-sensitivity detection of forensic drug panel in human whole blood
In a forensic laboratory, the ability to accurately quantify large panels of analytes in complex
biological matrices over a wide range of concentrations is challenging, and it often requires dilution of
the samples to bring them within the calibration range of the instrument. In this technical note, the
SCIEX 7500 system demonstrates exceptional performance in speed, linear dynamic range and sensitivity to
overcome this challenge, quantifying a panel of 49 drugs in human whole blood.
Ultra-sensitive analytical methodology for the quantification of
11-nor-9-carboxy-THC (THC-COOH) in oral fluid
The detection and quantification of THC in drug screening can result in a high number of false positives
since this analyte can be found in subjects passively exposed to cannabis. The SCIEX 7500 system offers
you high sensitivity, robustness and performance to accurately quantify pg/mL levels of THC-COOH in oral
fluid, without the need for derivatization.
Analysis of estrogens in plasma with rapid chromatography and reduced sample volume
The SCIEX 7500 System was used to analyze estrone, estradiol and estriol (the 3 Es) spiked in human plasma. Sensitivity showed a big improvement compared to previous generations of mass spectrometer, achieving sub-3pg/mL from only 200 μL of sample. Linear Dynamic Range was over 4 orders of magnitude, and reproducibility was demonstrated at trace concentrations (< 6.5% CV).
Significant gains in quantitative sensitivity using microflow chromatography
How much more sensitive are quantification workflows for peptides and small molecules on the 7500 System over the previous generation of instruments? Calibration curves were ran on three SCIEX 7500 Systems and three QTRAP 6500+ Systems, both with microflow LC, and the LLOQs and peak areas were compared. Both average peak area gains and LLOQ improvements were observed. Find out more details about 7500 System improvements with Micro LC.
Enabling new levels of quantification
The SCIEX 7500 System is the next high-end quantitative platform from SCIEX. D-Jet Ion Guide and integrated E-Lens Technology combine to deliver significant sensitivity gains over the previous generation of instruments. The OptiFlow Pro Ion Source, built on the reliability and efficiency of the legendary Turbo V Ion Source, now provides higher flexibility to meet diverse application needs with a single source solution. The modular design allows fast switching between high and low flow regimes, and adapts to workflow requirements (ESI vs. APCI ionization modes).
Robust bioanalysis over a large 1500 injection sample set
The SCIEX 7500 System provides excellent levels of robustness for bioanalysis from plasma matrices and provides consistent area values over more than two days of analytical run time. Analysis was performed in both positive and negative mode, highlighting that the SCIEX 7500 System is capable of high levels of robustness in both acquisition approaches. No cleaning or maintenance of the instrument was necessary after this analysis was completed.