Eliminate the Fear of Missing a Relevant Analyte
Reduce the risk that you’ve missed a critical component in your sample through the unbiased, comprehensive data acquisition strategy that delivers the data completeness your experiments demand.
SWATH Acquisition is a data-independent acquisition strategy, and when combined with the sensitivity and high-speed MS/MS acquisition enabled only by SCIEX TripleTOF and QTOF hardware, every detectable analyte in your sample is fragmented – this is true MS/MSALL. As the name suggests, you get a complete MS and MS/MS picture of everything detectable in your sample, without the need for repeated runs or re-analysis.
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Data-dependent vs. Data-independent acquisitions
How is SWATH different? Let’s explain.
Data Dependent Acquisition (DDA):
During an LC-MS analysis, there are many analytes eluting from your column over time and entering the mass spec. In data-dependent acquisition, an MS spectrum is collected on a broad m/z range, and the analyte peaks are detected and sorted by descending intensity. MS/MS acquisition is then triggered for the analytes starting at the top of the list. This is repeated over and over again across the LC gradient. Here a narrow Q1 isolation window is used to send only that analyte through for MS/MS analysis. But DDA has one critical limitation: when there are many analytes eluting at the same time and they are highly varied in abundance, there is a high risk of lower level analytes either not being detected in the original MS spectrum or the mass spec running out of time (too slow relative to the complexity of the sample) to capture MS/MS spectra for everything detected in MS mode. This creates gaps in your data.
Capture a Complete Digital Archive of your Sample in the First Analysis
Stop storing samples, and start storing data. SWATH Acquisition collects all the data you need on your sample the first time. This becomes especially critical when time is a factor, reanalysis is not an option, sample quantity is limited, or your sample is at risk to degrade.
As a data-independent acquisition strategy, SWATH Acquisition collects MS and MS/MS information on every detectable peak –leaving you with an option to re-interrogate your sample data should new questions arise tomorrow. Your digital archive is your safety net, allowing you to re-analyze your sample without having to re-run it on your mass spec. Consider SWATH Acquisition data like a biobank of comprehensive digital maps of your samples, without having to keep the samples themselves.
Reduce Laborious Method Development
Stop spending hours or days developing methods to run your samples and experiments. SWATH Acquisition utilizes generic MS acquisition settings, requiring little to no method development, so your time and resources are spent getting data and results.
Developing and optimizing targeted mass spec acquisition methods to optimally detect (and later quantify) your analytes of interest can be time-consuming, taking your time away from important work like actually running your samples and getting new exciting results. With minimal parameters to set-up and adjust, SWATH provides a single acquisition strategy that produces ultra-high quality quantitative data, without the hours of laborious method optimization.
How is SWATH Acquisition different? A typical MRM-based assay, as an example, requires you to optimize your method upfront to select for the compounds or MRM transitions you want to detect.
With SWATH Acquisition, a single generic MS acquisition method is used all of the time. This means you select your compounds of interest from the digital map AFTER you collect your comprehensive MS and MS/MS data on your sample.
But most importantly, should your data analysis present new questions, you can simply re-interrogate the data you’ve already collected rather than updating your acquisition method and re-analyzing your sample from step 1.
And once your SWATH Acquisition method is optimized for a sample type, the same method can be used for analysis across many similar samples no matter what analytes you're studying, saving you tremendous time.