CESI 8000 Plus system

See what you've been missing with CESI-MS

Experience the revolutionary ESI-MS approach to discovering previously undetectable characteristics from the minutest amount of precious samples, faster than ever before. Whether using as a standalone capillary electrophoresis instrument or in conjunction with the power of mass spectrometry, the CESI 8000 Plus system allows you to concentrate on your science rather than technology. You’ve worked hard to get your sample this far. Get the most from that precious material and hunt down the hidden information you couldn’t see before:

  • Intact mAb charge variants with online MS detection
  • Proteoforms and peptide PTMs such as those with multiple phosphorylation sites
  • Challenging charged and polar metabolites/degradation products such as anionic and/or hydrophilic analytes
  • Isobaric metabolites and glycans not resolved by traditional techniques
  • Protein conformation and quantitation
CESI 8000 Plus ESI-MS-High-Performance System
Key features

Zero dead volume

Single continuous separation capillary is contained within the OptiMS cartridge

Multi-detection capability

In stand-alone CE mode, UV/VIS, PDA, or LIF detection can be selected

Multiple injections from precious samples

Sample analysis from as little as 5 μL

Expand the reach of your mass spectrometer

The integration of capillary electrophoresis with electrospray ionization into a single dynamic process within the same device brings new capabilities to researchers.

Go beyond the possibilities

The CESI 8000 Plus system high-performance system delivers fast, efficient and unique separation selectivity with enhanced sensitivity, while simultaneously expanding coverage.

CE-MS: More info, less sample

Learn why capillary electrophoresis - mass spectrometry (CE-MS) is an attractive microscale analytical technique for addressing biological questions inherently dealing with low amounts of material.

Isobaric compounds

Simplify the analysis of isobaric compounds inadequately resolved with current methods.

Native conditions

Study the molecular interactions and structure of your proteins and peptides under native conditions.

See more than ever before

Expanded coverage allows you to see more than ever before.

Expand the coverage

Reveal hydrophilic charged and polar compounds and increase sensitivity in your metabolome and glycome analysis, not possible with LC methods.

Uncover new PTMs

Confidently quantify and characterize proteoform and peptide PTMs, such as those with multiple phosphorylation and glycosylation sites.

Separate isomers

Resolve isomers – unseeable with traditional LC-MS methods.

More dynamic, efficient processes

CESI-MS allows for a more robust system with ultra-low-flow < 10 nL/min, into the mass spectrometer resulting an overall increase in ionization efficiency.

Minute sample \consumption

Samples are often limited in quantity. CESI-MS has nL sample consumption, leaving most of the material available for analysis by orthogonal techniques.

Streamline your workflow

Use the same method and reagents for multi-level characterization of protein therapeutics. Switch between pos and neg ion modes without changing setup.

Frustration free

Open tube technology eliminates frustrations common with nanoscale techniques. No more minimizing dead volumes, or dealing with carryover and clogs.



29.2 in (74.2 cm) H x 25 in (63.5 cm) W x 28.4 in (72.1 cm) D

Portable lab bench

36 in x 30 in (91.4 cm x 76.2 cm)
Height adjusts 27 in to 44 in (69 - 112 cm)

Weight (uncrated)

188 lbs (85.3 kg)

Electrical requirements

Voltage: 100 - 240 V; 50/60 Hz

Sample trays

2 x 48 CESI vials / 0.3 mL microvials

Buffer tray

2 x 36 CESI vials

Pressure delivery range

-5 to +100 PSI

Required sample volume

5 μl when using NanoVials

The ultimate tool for high-resolution biotherapeutics separations

Downloads and resources

Filter by Type:

Charge variant analysis

Understanding the significance of sample preparation in studies of the nanoparticle metabolite corona using CESI-MS

CE-MS analysis of epigenetically active metabolites during in vitro chromatin assembly

Recent agricultural and natural products applications of CESI-MS

CE-MS in bottom-up and top-down proteomic and glycomic profiling of ng- and sub-ng-level samples

CESI-MS/MS for the analysis of highly polar pesticides

Ultrasensitive CESI-MS quantification of biomolecules based on chemical derivatization

The role of CESI for functional characterization of monoclonal antibodies

Capillary electrophoresis for top-down proteomics of proteoforms and their complexes

Native capillary electrophoresis-mass spectrometry of near 1 MDa multimeric protein assemblies

Towards single cell glycomics by CESI-MS

Identifying and Quantifying Aspartate and Iso-Aspartate Isomers and Other Difficult PTMs in Proteins

Enhanced identification of multiple phosphorylation sites using CESI-MS in comparison to nano LC-MS system using ESI

CZE Rapid Charge Variant Analysis kit flyer (ZH)

CE-MS solutions enabling next-generation therapeutics

CE Consumable Flyer

OptiMS Waters MS Adapter Installation Guide

OptiMS Thermo MS Adapter Installation Guide

CESI 8000 Plus High Performance Separation-ESI Module User Guide

CESI 8000 Plus System Administration Guide

OptiMS Bruker MS Adapter Installation Guide

CE instrument maintenance flyer

CESI 8000 High Performance Separation - ESI Module

CE-MS solutions enabling next-generation therapeutics

CE Training Overview 2021

CESI 8000 Plus Brochure

The Perfect Accessory for Your Mass Spectrometer

Capillary Electrophoresis kit brochure


LC-MS/MS 技术平台

SCIEX Capillary Electrophoresis Consumables flyer

CE Training Overview DE 2021

CZE Rapid Charge Variant Analysis kit flyer

OptiMS Adapter Brochure

Comprehensive Analysis of Recombinant Human Erythropoietin Glycoforms by Capillary Electrophoresis and Nanoflow Liquid Chromatography Coupled with Middle-down Mass Spectrometry

The application of Capillary Electrospray Ionization with negative ion electrospray ionization for the analysis of plant metabolites

Ultra-sensitive host cell protein detection using CESI-MS with SWATH® Acquisition

From small to very large: orthogonal, sensitive polar molecule analysis by CESI-MS

Comprehensive Characterization of Trastuzumab using a Single-Shot Peptide Mapping Approach with CESI-MS

The Use of Capillary Electrophoresis Coupled to Mass Spectrometry (CESI-MS) for Quantitation of Nucleotides and Nucleosides

The effect of ultra-low flow on the ionization of biotherapeutics

Slide 1

Kein Folientitel

Microsoft PowerPoint - ASMS 2014 HL.pptx

Microsoft PowerPoint - CE Pharm 2010 Poster - Hudson.ppt

Diapositive 1

Kein Folientitel

56x36 Poster Template

Characterization of Intact Prostate Specific Antigen (PSA) and Its proteoforms by CESI-MS under Native and Denaturing Conditions

Slide 1

Native state charge variant analysis of commercialized monoclonal antibodies poster

Evaluating the Addition of Supercharging Reagents During CESI-MS for Identification and Characterization of Biopharmaceuticals

Charge heterogeneity characterization of next-generation antibodies using capillary electrophoresis and mass spectrometry

7 Detecting-Challenging.indd

Comprehensive Analysis of Recombinant Human Erythropoietin Glycoforms by Capillary Electrophoresis and Nanoflow Liquid Chromatography Coupled with Intact/Middle-Down Mass Spectrometry

Ionization Efficiency, Ion Suppression and Detection Sensitivity of CESI-MS

A New Approach to the Analysis of Anionic Metabolites by CESI-MS with Negative Ion Electrospray Ionization

High resolution approach to the detection of insulin degradation products

Optimized digestion procedure and characterization for monoclonal antibodies and proteins by CESI-MS

Neutral OptiMS Capillary Data Sheet

CESI-MS comparison of tryptic diges an assessment of bio similarity

Improving identification and quantification of polar herbicides by CESI-MS

Improving the Detection Limits for Highly Basic Neuropeptides Using CESI-MS

CESI-MS Analysis of Biofluids of Basic Drugs and Metabolites

Comprehensive Analysis of Low Abundant Mannose Glycopeptides in Peptide Mapping of Adalimumab

Compact Quantitative Proteomics Workflow Combining SILAC Labeling, Chromatographic Pre-fractionation and CESI-MS with a Neutral Capillary Surface

Analysis of Anionic Metabolites by CESI-MS

CESI-MS performance check and MS calibration using PepCalMix standard for silica surface OptiMS cartridges

Sensitive Glycoform Profiling of Interferon-beta-1a (Avonex) and Recombinant Human Erythropoietin by CESI-TOF-MS

A New High-resolution Approach to the Detection of Insulin Degradation Products

Intact Casein - A Model System for the Separation of Intact Phosphorylated Proteins by CESI-MS

Separation and Analysis of Intact Prostate Specific Antigen (PSA) and its Proteoforms by CESI-MS Under Native and Denaturing Conditions.

Preparation scale-up of complex biological samples for deep N-glycomic analysis by CE-LIF and CESI-MS

Ultra-sensitive host cell protein detection using CESI-MS with SWATH acquisition

A new approach to the analysis ofanionicmetabolites by CESI-MS with negative ion electrospray ionization

Analysis of Intact MUPs (Major Urinary Proteins) from Mice by CESI-MS

Structural Characterization of Antibody Drug Conjugates (ADCs) by a Combination of Intact, Middle-up and Bottom-up Approaches using CESI-MS

Characterization of Phospho- and Glycopeptides by CESI-MS: A Powerful Technique for Increasing Sensitivity

Quantitation of Nucleotides and Nucleosides without derivatization using CESI-MS

Charge Variant Assessment of Nanobodies at the Intact Level by CESI-MS

Increasing Throughput with Multi-Segment-Injection-Capillary Electrospray Ionization-Mass Spectrometry (MSI-CESI-MS)

Velocity-corrected area calculation: SCEX PA 800 plus system Empower driver version 1.3 vs. 32 Karat software

CESI-MS of Intact Proteins

Expanding phosphorylation and post translational site mapping in proteomics using CESI-MS

Comprehensive Analysis of Recombinant Human Erythropoietin Glycoforms by Capillary Electrophoresis and Nanoflow Liquid Chromatography Coupled with Intact/Middle-Down Mass Spectrometry

SDS-MW calibration and molecular weight estimation using the SCIEX PA 800 Plus Empower driver version 1.3

Improved CESI-MS sensitivity and repeatability in glycopeptide analysis using a dopant enriched nitrogen gas

Chiral Analysis of Methamphetamine and Its Metabolite, Amphetamine in Urine by CESI-MS

A covalent, cationic polymer coating method for the CESI-MS analysis of intact proteins and polypeptides

Analyzing the average degree of substitution of betadex sulfobutyl ether sodium by capillary electrophoresis (ZH)

Analysis of purity and isoelectric points of HPV by capillary electrophoresis (ZH)

Analysis of Purity and Integrity of mRNA Vaccine by Capillary Gel Electrophoresis (ZH)

Highly sensitive CE-SDS-LIF purity analysis of T7 RNA polymerase (ZH)

Purity analysis of oligonucleotides by capillary gel electrophoresis (ZH)

Analysis of Poly A tail distribution of mRNA by capillary gel electrophoresis (ZH)

High Throughput Detection of IgG Purity by Using Quick Rinse CE-SDS Separation Method


SCIEX pH 6.5 sample buffer: Specified by Chinese Pharmacopoeia for CE-SDS analysis (Chinese)


Analysis of size variants and isoelectric point of recombinant human serum albumin by capillary electrophoresis (ZH)

Size analysis of residual host cell DNA in Recombinant protein vaccine by CGE-LIF (ZH)

Separation, identification and quantification of associated impurities in cobratide using sheathless CE-MS and CE-UV (Chinese)


Analysis of Components in Shengmai Injection by CESI-MS/MS and LC-MS/MS (Chinese)

Determination of P-carboxybenzaldehyde and P-methyl benzoic acid in P-terephthalic acid by capillary electrophoresis (ZH)

Quality control of raw materials of diagnostic reagent in vitro by capillary electrophoresis (ZH)

Analysis of components in Shengmai injection by CESI-MS (Chinese)

Quantification of whey protein content in infant formula by CE-SDS (ZH)

Multi-laboratory validation for the purity analysis of protein products in CE-SDS method using CESI 8000 Plus (ZH)

The determination of α-lactalbumin and β-lactoglobulin in dairy by capillary zone electrophoresis (ZH)

Purity analysis of MRNA vaccine by using CGE-LIF (ZH)

Analysis of glycopeptides in erythropoietin by CESI-MS/MS and NanoLC-MS/MS techniques (Chinese)

The determination of lactoferrin in dairy by capillary zone electrophoresis (ZH)