CESI 8000 Plus ESI-MS System

See What You've Been Missing with CESI-MS

Experience the revolutionary ESI-MS approach to discovering previously undetectable characteristics from the minutest amount of precious samples, faster than ever before. Whether using as a standalone capillary electrophoresis instrument or in conjunction with the power of mass spectrometry, the CESI 8000 Plus allows you to concentrate on your science rather than technology. You’ve worked hard to get your sample this far. Get the most from that precious material and hunt down the hidden information you couldn’t see before:

  • Intact mAb charge variants with online MS detection
  • Proteoforms & peptide PTMs such as those with multiple phosphorylation sites
  • Challenging charged & polar metabolites/degradation products such as anionic and/or hydrophilic analytes
  • Isobaric metabolites and glycans not resolved by traditional techniques
  • Protein conformation and quantitation
CESI 8000 Plus ESI-MS-High-Performance System
Key Features

Zero Dead Volume

Single continuous separation capillary is contained within the OptiMS cartridge

Multi-Detection Capability

In stand-alone CE mode, UV/VIS, PDA, or LIF detection can be selected

Multiple Injections from Precious Samples

Sample analysis from as little as 5 μL

Expand the Reach of Your Mass Spectrometer

The integration of Capillary Electrophoresis with Electrospray Ionization into a single dynamic process within the same device brings new capabilities to researchers.

Go Beyond the Possibilities

The CESI 8000 Plus high-performance system delivers fast, efficient and unique separation selectivity with enhanced sensitivity, while simultaneously expanding coverage.

CE-MS: More Info, Less Sample

Learn why capillary electrophoresis - mass spectrometry (CE-MS) is an attractive microscale analytical technique for addressing biological questions inherently dealing with low amounts of material.

Isobaric Compounds

Simplify the analysis of isobaric compounds inadequately resolved with current methods.

Native Conditions

Study the molecular interactions and structure of your proteins and peptides under native conditions.

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See More Than Ever Before

Expanded coverage allows you to see more than ever before.

Expand the Coverage

Reveal hydrophilic charged and polar compounds and increase sensitivity in your metabolome and glycome analysis, not possible with LC methods.

Uncover New PTMs

Confidently quantify and characterize proteoform and peptide PTMs, such as those with multiple phosphorylation and glycosylation sites.

Separate Isomers

Resolve isomers – unseeable with traditional LC-MS methods.

More Dynamic, Efficient Processes

CESI-MS allows for a more robust system with ultra-low-flow < 10 nL/min, into the mass spectrometer resulting an overall increase in ionization efficiency.

Minute Sample Consumption

Samples are often limited in quantity. CESI-MS has nL sample consumption, leaving most of the material available for analysis by orthogonal techniques.

Streamline Your Workflow

Use the same method and reagents for multi-level characterization of protein therapeutics. Switch between pos and neg ion modes without changing setup.

Frustration Free

Open tube technology eliminates frustrations common with nanoscale techniques. No more minimizing dead volumes, or dealing with carryover and clogs.

Key Features


29.2 in (74.2 cm) H x 25 in (63.5 cm) W x 28.4 in (72.1 cm) D

Portable Lab Bench

36 in x 30 in (91.4 cm x 76.2 cm)
Height adjusts 27 in to 44 in (69 - 112 cm)

Weight (uncrated)

188 lbs (85.3 kg)

Electrical Requirements

Voltage: 100 - 240 V; 50/60 Hz

Sample Trays

2 x 48 CESI vials / 0.3 mL microvials

Buffer Tray

2 x 36 CESI vials

Pressure Delivery Range

-5 to +100 PSI

Required Sample Volume

5 μl when using NanoVias

The Ultimate Tool for High-Resolution Biotherapeutics Separations

See Why There’s Nothing Else Like the CESI 8000 Plus


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Empower your PA 800 Plus with the SCIEX Empower Driver version 1.3

Take control of your PA 800 Plus with Waters Empower 3 Chromatography Data Software (CDS) for data integrity compliance. Reduce regulatory risk and maximize method transfer efficiency with the newly released SCIEX Empower Driver version 1.3.

Downloads & Resources

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CESI 8000 Plus High Performance Separation-ESI Module Site Planning Guide

Analyzing the Average Degree of Substitution of Betadex Sulfobutyl Ether Sodium by Capillary electrophoresis (ZH)

Analysis of purity and isoelectric points of HPV by capillary electrophoresis


Abstract-Analysis of components in Shengmai injection by CESI-MS

Quantification of Whey Protein Content in Infant Formula by CE-SDS (ZH)

Multi-Laboratory Validation for the Purity Analysis of Protein Products in CE-SDS Method Using CESI 8000 Plus

The Determination of α-lactalbumin and β-lactoglobulin in Dairy by Capillary Zone Electrophoresis (ZH)

Purity Analysis of mRNA Vaccine by Using CGE-LIF (ZH)

Analysis of glycopeptides in erythropoietin by CESI-MS/MS and NanoLC-MS/MS techniques

The Determination of Lactoferrin in Dairy by Capillary Zone Electrophoresis (ZH)

5 分钟轻松完成电荷异质性分析

CE-MS solutions enabling next-generation therapeutics

CE Instrument Maintenance

CE Training Overview 2021

精简、全面的 LC-MS 多质量属性监控(MAM) 工作流程

SCIEX Capillary Electrophoresis Consumables Guide

CE Training Overview DE 2021

Comprehensive Analysis of Recombinant Human Erythropoietin Glycoforms by Capillary Electrophoresis and Nanoflow Liquid Chromatography Coupled with Middle-down Mass Spectrometry

Microsoft PowerPoint - Fonslow 2017 ABRF General CESI-MS Poster Final - legal approved (1)

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Microsoft PowerPoint - ASMS 2014 HL.pptx

Microsoft PowerPoint - CE Pharm 2010 Poster - Hudson.ppt

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High resolution approach to the detection of insulin degradation products

Optimized digestion procedure and characterization for monoclonal antibodies and proteins by CESI-MS

Neutral OptiMS Capillary Data Sheet

CESI-MS comparison of tryptic diges an assessment of bio similarity

Improving Identification and Quantification of Polar Herbicides by CESI-MS

B2011-12537 Drugs and Metabolites White Paper.indd

15 quantitative_Proteomics_workflow_Lindner.indd

Analysis of Anionic Metabolites by CESI-MS

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Separation and Analysis of Intact Prostate Specific Antigen (PSA) and its Proteoforms by CESI-MS Under Native and Denaturing Conditions.

Preparation Scale-Up Of Complex BiologicakSamples For Deep N-Glycomic Analysis By CE-LIF and CESI-MS

Microsoft Word - Host cell protein detection using CESI-MS with SWATH Tech Note.docx

A new approach to the analysis ofanionicmetabolites by CESI-MS with negative ion electrospray ionization

8 Charge-Variant-Assessment-of-Nanobodies-at-the-Intact-Level-by-CESI-MS.indd

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Velocity-corrected area calculation SCIEX PA 800 Plus Empower Driver version 1.3 vs. 32 Karat Software.docx

CESI-MS of Intact Proteins

Comprehensive Analysis of Recombinant Human Erythropoietin Glycoforms by Capillary Electrophoresis and Nanoflow Liquid Chromatography Coupled with Intact/Middle-Down Mass Spectrometry

SDS-MW calibration and molecular weight estimation using the SCIEX PA 800 Plus Empower Driver version 1.3

Microsoft Word - DEN GAS LUMC app note 1_0_GSM_mw corrections accepted (3)

Chiral Analysis of Methamphetamine and Its Metabolite, Amphetamine in Urine by CESI-MS

A covalent, cationic polymer coating method for the CESI-MS analysis of intact proteins and polypeptides

Identifying and Quantifying Aspartate and Iso-Aspartate Isomers and Other Difficult PTMs in Proteins

Charge variant analysis

Enhanced identification of multiple phosphorylation sites using CESI-MS in comparison to nano LC-MS system using ESI

CE-MS solutions enabling next-generation therapeutics

Ultra-sensitive host cell protein detection using CESI-MS with SWATH® Acquisition

The application of Capillary Electrospray Ionization with negative ion electrospray ionization for the analysis of plant metabolites

Capillary electrophoresis kits

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Improving the Detection Limits for Highly Basic Neuropeptides Using CESI-MS

Intact Casein - A Model System for the Separation of Intact Phosphorylated Proteins by CESI-MS

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Expanding phosphorylation and post translational site mapping in proteomics using CESI-MS

CESI-MS performance check and MS calibration using PepCalMix standard for silica surface OptiMS cartridges

Comprehensive Analysis of Recombinant Human Erythropoietin Glycoforms by Capillary Electrophoresis and Nanoflow Liquid Chromatography Coupled with Intact/Middle-Down Mass Spectrometry

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LC-MS/MS 技术平台

Comprehensive Analysis of Low Abundant Mannose Glycopeptides in Peptide Mapping of Adalimumab

Analysis of Intact MUPs (Major Urinary Proteins) from Mice by CESI-MS

Structural Characterization of Antibody Drug Conjugates Using CESI-MS | SCIEX

Characterization of Phospho- and Glycopeptides by CESI-MS: A Powerful Technique for Increasing Sensitivity

CESI 8000 High Performance Separation - ESI Module

SCIEX CZE Rapid Charge Variant Analysis Kit