Characterize therapeutic molecules with confidence on the PA 800 Plus system

Perform consistent and compliant biotherapeutics characterization faster than ever

With the PA 800 Plus system, you can confidently safeguard the success of your biologics, run multiple characterizations from a single system that biopharma labs depend on, and produce qualitative and quantitative analyses with speed and confidence.

The PA 800 Plus system is a robust analytical, kit-based platform that can help you with:

  • Monoclonal antibody and next-generation protein applications
    • Purity/Heterogeneity with gold standard CE-SDS in minutes
    • Glycan analysis with award-winning Fast Glycan technology
    • Charge heterogeneity with fast and simple CZE or with hi-res cIEF
  • Gene therapy and nucleic acid applications
    • mRNA purity and integrity analysis
    • Capsid protein purity
    • AAV Empty vs. Full
    • Plasmid and linear double-stranded DNA (dsDNA) analysis
    • Small RNA or DNA analysis
  • Cell therapy
PA 800 Plus Pharmaceutical Analysis System
Key features

Multiple applications, one platform

Run multiple characterizations from a single system to improve your operational efficiency and effectiveness.

Raw unmanipulated data

Have the confidence critical to both you and regulatory agencies for product submission and commercialization with raw, unmanipulated data.

Reproducibility matters

Get repeatable results with exclusive recirculating liquid coolant to control separation temperature precisely.

Portability matters

Easily transfer methods using robust, automated assays with proven portability ‐ ideal for global multi-user, multi-instrument.

Precision analytics for oligonucleotide, cell and gene therapy

As the technologies for manufacturing and analysis of gene therapy products continue to evolve, it’s important to keep up to date on growing industry trends and analytical advancements. This compendium can help advance your gene therapies and oligonucleotide therapeutics to the next level with the latest tools and methods.

Download your copy of the compendium  

Multiple biotherapeutic characterizations on one platform

Biologics characterization results you can trust

Characterize molecules, large or small, with industry and regulatory-compliant capillary electrophoresis (CE) technology. It’s why every biopharmaceutical company with therapeutic IgGs currently on the market uses SCIEX CE systems in their bioanalytical processes.

Transferability

Develop and lock-down methods applicable to QC or regulated environments. Easily transfer methods using robust, automated assays with proven portability. Perfect for the global multi-user and multi-instrument organizations.

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EZ-CE cartridge

The convenient EZ-CE cartridge is ready-to-use across multiple applications. Enhance data consistency and minimize staff training with the PA 800 Plus EZ-CE cartridge.

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Trusted, fast and accurate biotherapeutic characterization

Industry and regulatory proven CE technology

The PA 800 Plus system is a robust analytical platform so you can have confidence in your data, without sacrificing speed.

Increase efficiency

Running multiple assays on one platform simplifies staff training, maximizes ROI and reduces laboratory footprint.

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Trust your assays

Extensive intercompany studies demonstrate the robustness of SCIEX capillary electrophoresis assays, encompassing CE-SDS, cIEF, CZE, and glycan analysis.

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Simplify compliance

The USP has included validated procedures and system suitability criteria for IgG purity and heterogeneity analysis by capillary electrophoresis.

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Maximize sensitivity

Achieve as low as 0.01% impurity detection, with at least three orders of magnitude of impurity, with modular detection (UV, PDA, LIF)

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You can do all this on your PA 800 Plus system

Automated and quantitative analysis of biotherapeutics

Generate highly reproducible data with PA 800 Plus system quantitative and automated solutions. Our engineers have collaborated closely with biopharma development and QC groups experienced in biologics characterization to create assays that address your analytical challenges.

Protein purity analysis

Quantitative protein purity analysis with SDS-gel capillary electrophoresis

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Glycan analysis

Run glycans faster than HILIC with simplified sample preparation, rapid, high-resolution separations, and automated glycan identification.

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Charge heterogeneity

Conduct advanced capillary isoelectric focusing (cIEF) and hi-speed capillary zone electrophoresis (CZE) for charge heterogeneity analysis.

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Peptide/Protein characterization

Analyze peptide and protein biotherapeutics beyond mAbs.

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Gene therapy and nucleic acid analysis

Analyze a variety of single-stranded and double-stranded oligos and fragments with kit-based solutions.

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Run glycans faster than HILIC

Watch Video

Glycoprotein to identified glycans within record 90 minutes

SCIEX Fast Glycan labeling and analysis delivers rapid glycan heterogeneity identification, capable of profiling your glycans in record time. Read all about our Fast Glycan technology.

Video Length

Empower your PA 800 Plus system with the SCIEX Empower Driver version 1.3

Take control of your PA 800 Plus system with Waters Empower 3 chromatography data software (CDS) for data integrity compliance. Reduce regulatory risk and maximize method transfer efficiency with the newly released SCIEX Empower Driver version 1.3.

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Streamline workflows with the Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)

Boost your laboratory's productivity by seamlessly integrating it into a single software solution. Take control of your PA 800 Plus system with the Chromeleon driver and unleash next-level performance with powerful processing, customizable reporting, and robust compliance tools.

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Windows 10 upgrade

We are now Windows 10 compatible! Upgrade your SCIEX CE instruments today.

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Related products

Kit-based assays

Get the reagents you need to run your experiments with one of the broadest selections of kit-based assays.

Learn more

EZ-CE cartridge

The convenient EZ-CE cartridge is ready-to-use across multiple applications.

Learn more

Connect to a mass spec

Integrate CE with electrospray ionization (ESI) and connect to a mass spec with the CESI 8000 Plus system.

Learn more
Specifications 
PA 800 Plus system (P/N: A66528)Includes separation module with UV, photodiode array and LIF detection and system controller with PA 800 Plus software.
PA 800S Plus system (P/N: A66527)Includes separation module with photodiode array detection and system controller with PA 800 Plus software.
DimensionsHeight: 29.2 in (74.2 cm)
Door open: 38.8 in (98.6 cm)
Width: 25 in (63.5 cm)
Depth: 28.4 in (72.1 cm)
Weight (uncrated)188 lbs (85.3 kg) (includes photodiode array detection)
Electrical requirementsVoltage: 100 - 240 V; 50/60 Hz
Voltage range1 to 30 kV programmable at 0.1 kV increments
Current range3 to 300 μA programmable at 0.1 μA increments
Pressure delivery range-5 to +100 PSI
Sample temperature control4 - 60°C
Capillary temperature control15 - 60°C
Sample trays: 2 x 96-well plates2 x 48 universal vials 2 x 48 0.2 mL microvials
Buffer tray2 x 36 universal vials
Detection capabilityUV/Vis
200, 214, 254, 280 nm standard filter
190 - 600 nm (custom filter option)
Diode array
190 - 600 nm (programmable)
0.5 - 32 Hz scan collection frequency (programmable)
Laser induced fluorescence (LIF)
300 - 700 nm excitation range
350 - 750 nm emission range
0 - 1000 RFU
Source lasers with 3 mW power output (P/N: A66528)488 nm solid-state laser (included in P/N: A66528)

Resources

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RNA 9000 purity and integrity kit methods

Characterization of lentiviral vectors using capillary electrophoresis platform technology webinar

High-throughput LNP-mRNA Integrity Profiling webinar

High-throughput LNP-mRNA integrity profiling webinar

Proven answers for protein, vaccine and gene therapy analysis webinar

Sample Preparation for the Fast Glycan Labeling and Analysis kit on the PA 800 Plus system video

Viral vector characterization: empty/full, transgene integrity and capsid protein analyses on a single platform webinar

Drug antibody ratio (ADC) calculations made easy and simple webinar

Genome integrity analysis of adeno-associated viruses (AAV) by genome sizing methodology webinar

How are CE methods being adapted to analyze new modalities webinar

Tips and Tricks for using the PA 800 Plus (JA) video

Best practices for using the PA 800 Plus with CE-SDS (JA) video

Peptide therapeutics analysis by Capillary Electrophoresis

Comprehensive method development for a wide size range of RNA

Pre-Assembled Cartridge flyer

Low pH SDS sample buffer flyer (ZH)

ruo-mkt-07-14191-a-rna-kit-flyer_V1_08-23

CZE Rapid Charge Variant Analysis kit flyer (ZH)

CE Consumable Flyer

PA 800 Plus system start-up consumable groups flyer

PA 800 Plus system Empower driver notes de version (FR)

PA 800 Plus system Empower driver notas de la versión (ES)

PA 800 Plus system Empower driver 版本发布说明 (ZH)

PA 800 Plus system Empower driver リリースノート (JA)

PA 800 Plus system Empower driver note di rilascio (IT)

PA 800 Plus system Empower driver versionshinweise (DE)

PA 800 Plus system Empower driver release notes

PA 800 Plus system Empower driver user guide

PA 800 Plus system system maintenance guide

PA 800 Plus system maintenance guide (FR)

PA 800 Plus system Empower driver user guide (DE)

PA 800 Plus system maintenance guide (ES)

PA 800 Plus system Empower driver user guide (ES)

PA 800 Plus system software validation guide

PA 800 Plus system Empower driver user guide (IT)

PA 800 Plus system Empower driver user guide (JA)

PA 800 Plus system Empower driver user guide (ZH)

PA 800 Plus system Empower driver user guide (FR)

PA 800 Plus system IgG Purity and Heterogeneity Assay kit application guide

PA 800 Plus system LIFluor™ EnhanCE dsDNA 1000 Dye application guide (JA)

PA 800 Plus system Fast Glycan Labeling and Analysis kit application guide

PA 800 Plus system IgG Purity and Heterogeneity Assay kit application guide (JA)

PA 800 Plus system IgG Purity and Heterogeneity Assay kit application guide (ZH)

PA 800 Plus system SDS-MW Analysis kit application guide

PA 800 Plus system ssDNA 100-R kit application guide (ZH)

PA 800 Plus system dsDNA 1000 kit application guide

PA 800 Plus system CZE Rapid Charge Variant Analysis kit application guide (ZH)

PA 800 Plus system dsDNA 1000 kit application guide (JA)

PA 800 Plus system Capillary Isoelectric Focusing (cIEF) Analysis kit application guide

PA 800 Plus system ssDNA 100-R kit application guide

PA 800 Plus system Capillary Isoelectric Focusing (cIEF) Analysis kit application guide (JA)

PA 800 Plus system SDS-MW Analysis kit application guide (JA)

PA 800 Plus system LIFluor™ EnhanCE dsDNA 1000 Dye application guide (ZH)

PA 800 Plus system CZE Rapid Charge Variant Analysis kit application guide

PA 800 Plus system SDS-MW Analysis kit application guide (ZH)

PA 800 Plus system LIFluor™ EnhanCE dsDNA 1000 Dye application guide

PA 800 Plus system ssDNA 100-R kit application guide (JA)

PA 800 Plus system Capillary Isoelectric Focusing (cIEF) Analysis kit application guide (ZH)

PA 800 Plus system CZE Rapid Charge Variant Analysis kit application guide (JA)

PA 800 Plus system RNA 9000 Purity and Integrity kit application guide

PA 800 Plus system dsDNA 1000 kit application guide (ZH)

CE Grade Water flyer

CE instrument maintenance flyer

PA 800 Plus system brochure (ES)

SCIEX solutions for LNP-based genetic medicines brochure

CE-SDS training flyer

CE Training Overview 2021

Capillary electrophoresis Windows 10 flyer

Fast Glycan labeling and analysis kit flyer

Fast Glycan Labeling and Analysis kit flyer

Capillary electrophoresis consulting flyer

Capillary Electrophoresis kit brochure

Low pH phosphate SDS sample buffer flyer (ZH)

LC-MS/MS 技术平台

Capillary electrophoresis solutions for DNA/mRNA vaccines flyer (ZH)

PA 800 Plus system Empower driver flyer (ZH)

SCIEX Capillary Electrophoresis Consumables flyer

Low pH SDS sample buffer flyer

SCIEX solutions for gene therapy and oligonucleotide compendium

CE Training Overview DE 2021

Capillary electrophoresis training course flyer

CZE Rapid Charge Variant Analysis kit flyer

A holistic approach to understanding cIEF poster

Fully automated sample preparation with ultrafast N-glycosylation analysis of therapeutic antibodies poster

A sensitive and robust plasmid analysis method by capillary electrophoresis-laser induced fluorescence (Pfizer and SCIEX) poster

CZE Method

CZE Capillary Conditioning

CZE Shutdown

CZE Rapid Charge Variant Analysis kit methods

CZE Analysis

Low-level quantitation of impurities in bispecific proteins using CE-SDS with LIF detection

High-precision lentivirus titer determination and protein profiling

Size distribution analysis of residual host cell DNA fragments in lentivirus by CGE-LIF

Rapid CZE analysis of erythropoietin variants

Sensitive impurity analysis of AAV capsid proteins by CE using Chromeo P503 fluorescent (ES)

Capillary electrophoresis in quality control: Part I: Application for therapeutic proteins

Assessing glycosimilarity of biotherapeutics

Simple, fast and robust method for plasmid purity testing and degradation monitoring

High efficiency evaporative fluorophore labeling of glycans

Achieving robust cIEF analysis of monoclonal antibodies while increasing capillary run-life and maintaining high resolution and reproducibility

Accurate analysis of double stranded DNA over an extended size range

Assay of IgG purity and heterogeneity using high-resolution sodium dodecyl sulfate capillary gel electrophoresis

Determination of full, partial and empty capsid ratios for adeno-associated virus (AAV) analysis

Sensitive AAV capsid protein impurity analysis by CE using P503 dye

Preparation scale-up of complex biological samples for deep N-glycomic analysis by CE-LIF and CESI-MS

IgG purity/heterogeneity and SDS-MW assays with 96-well plate adaption

Purity analysis of adeno-associated virus (AAV) capsid proteins using CE-SDS method

Native state charge variant analysis of commercialized monoclonal antibodies in minutes

Genome sizing methodology for genome integrity analysis of adeno-associated viruses (AAV)

Automation of CE-SDS for IgG purity/heterogeniety

Comparative characterization of the Fc domain N-glycosylation in monoclonal antibody and fusion protein therapeutics

Ultrafast Analysis of Human Milk Oligosaccharides (HMOs)

Capillary electrophoresis in quality control: Part II: CE-SDS: Method development and robustness

Separation of recombinant human erythropoietin (rhEPO) using the European Pharmacopoeia method on the PA 800 plus system

Simplifying CE-SDS data processing with mineral oil

Velocity-corrected area calculation: SCEX PA 800 plus system Empower driver version 1.3 vs. 32 Karat software

Fast glycan sequencing using a fully automated carbohydrate sequencer

Purity analysis of adeno-associated virus (AAV) capsid proteins using CE-LIF technology

Evaluation of the glycosimilarity of biotherapeutics (ES)

Optimize non-reduced CE-SDS analysis with the SCIEX low pH SDS sample buffer

SDS-MW calibration and molecular weight estimation using the SCIEX PA 800 Plus Empower driver version 1.3

Optimization of a robust and sensitive capillary electrophoresis laser induced flourescence method for monitoring plasmid stability and purity

High-Resolution cIEF of Therapeutic Monoclonal Antibodies

A Series of Collaborations Between Various Pharmaceutical Companies and Regulatory Authorities Using CE

A single analytical platform for glycan analysis, charge heterogeneity, and purity determination of the NIST mAb

Rapid CZE analysis of erythropoietin variants (ZH)

Heterogeneity analysis of cysteine-linked antibody-drug conjugate by CE method (ZH)

Analyzing the average degree of substitution of betadex sulfobutyl ether sodium by capillary electrophoresis (ZH)

Analysis of purity and isoelectric points of HPV by capillary electrophoresis (ZH)

Purity analysis of CircRNA vaccine by CGE-LIF method (ZH)

Analysis of capping efficiency of MRNA by capillary gel electrophoresis (ZH)

Analysis of Purity and Integrity of mRNA Vaccine by Capillary Gel Electrophoresis (ZH)

Size analysis of residual host cell DNA in Lentivirus by CGE-LIF

Highly sensitive CE-SDS-LIF purity analysis of T7 RNA polymerase (ZH)

Purity analysis of oligonucleotides by capillary gel electrophoresis (ZH)

Analysis of Poly A tail distribution of mRNA by capillary gel electrophoresis (ZH)

High Throughput Detection of IgG Purity by Using Quick Rinse CE-SDS Separation Method

Identification and quantitative analysis of impurity E in glutathione by capillary electrophoresis (ZH)

Rapid purity analysis of plasmid using neutral coated capillary in CGE-LIF method (ZH)

Analysis of size variants and isoelectric point of recombinant human serum albumin by capillary electrophoresis (ZH)

Determination of free carrier proteins in polysaccharideprotein conjugate vaccines by capillary micellar electrokinetic chromatography (ZH)

Purity analysis of monoclonal antibodies (5-TAMRA labeling) by CE-SDS-LIF (ZH)

SCIEX CE solutions in China Pharmacopoeia (ZH)

Determination of P-carboxybenzaldehyde and P-methyl benzoic acid in P-terephthalic acid by capillary electrophoresis (ZH)

Quality control of raw materials of diagnostic reagent in vitro by capillary electrophoresis (ZH)

Quantification of whey protein content in infant formula by CE-SDS (ZH)

Multi-laboratory validation for the purity analysis of protein products in CE-SDS method using CESI 8000 Plus (ZH)

The determination of α-lactalbumin and β-lactoglobulin in dairy by capillary zone electrophoresis (ZH)

Purity analysis of MRNA vaccine by using CGE-LIF (ZH)

Comparation the N-Glycan of different sources recombinant human erythropoietin by using capillary electrophoresis with laser-induced fluorescence detector (ZH)

The determination of lactoferrin in dairy by capillary zone electrophoresis (ZH)

Isoelectric points analysis of LNP encapsulated mRNA by cIEF (ZH)

Empower Your PA 800 Plus with Waters Empower3 Enterprise Software

A Robust cIEF Method: Intermediate Precision

Identification of System Parameters Critical for High-Performance cIEF

Native state charge variant analysis of commercialized monoclonal antibodies poster

Size analysis of residual host cell DNA in Recombinant protein vaccine by CGE-LIF (ZH)

Study on the stability of RNA Analysis by neutral capillary combined with RNA 9000 Kit (ZH)

Purity analysis of SgRNA by capillary gel electrophoresis (ZH)

Development of purity analysis methods for synthetic prime editing guide RNAs (pegRNAs) with high-level secondary structures for prime editing research applications

Masterclass LIVE: Characterization of engineered Cas9 protein and its post translational modifications - Webinar on-demand

Proven answers for Protein, Vaccine and Nucleotide Characterization

PA 800 Plus system RNA 9000 Purity & Integrityキット アプリケーションガイド

PA 800 Plus system RNA 9000 Purity & Integrity 试剂盒 应用指南

PA 800 Plus Pharmaceutical Analysis System - System Administration Guide

PA 800 Plus System Overview Guide (EN)

Challenges with CE-SDS?

CE consumables and reagents catalog

CGE-LIF方法分析不同工艺阶段狂犬疫苗中宿主残留DNA的片段大小分布

十六烷基磺酸钠-毛细管凝胶电泳法用于双抗的纯度分析

Learning Center 
Installing the LIF aperture and probe guide/Stabilizer step-by-step Video GuideWatch
Cartridge removal, rebuild and reinstallation step-by-step video guideWatch
Change the coolant tubing step-by-step video guideWatch
Change a detector step-by-step video guideWatch
Capillary removal step-by-step video guideWatch
Capillary storage step-by-step video guideWatch
Fast Glycan softwareDownload
How to maximize your capillary life flyerDownload

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