Characterize therapeutic molecules with confidence on the PA 800 Plus system

Perform consistent and compliant biotherapeutics characterization faster than ever

With the PA 800 Plus system, you can confidently safeguard the success of your biologics, run multiple characterizations from a single system that biopharma labs depend on, and produce qualitative and quantitative analyses with speed and confidence.

The PA 800 Plus system is a robust analytical, kit-based platform that can help you with:

  • Monoclonal antibody and next-generation protein applications
    • Purity/Heterogeneity with gold standard CE-SDS in minutes
    • Glycan analysis with award-winning Fast Glycan technology
    • Charge heterogeneity with fast and simple CZE or with hi-res cIEF
  • Gene therapy and nucleic acid applications
    • mRNA purity and integrity analysis
    • Capsid protein purity
    • AAV Empty vs. Full
    • Plasmid and linear double-stranded DNA (dsDNA) analysis
    • Small RNA or DNA analysis
  • Cell therapy
PA 800 Plus Pharmaceutical Analysis System
Key features

Multiple applications, one platform

Run multiple characterizations from a single system to improve your operational efficiency and effectiveness.

Raw unmanipulated data

Have the confidence critical to both you and regulatory agencies for product submission and commercialization with raw, unmanipulated data.

Reproducibility matters

Get repeatable results with exclusive recirculating liquid coolant to control separation temperature precisely.

Portability matters

Easily transfer methods using robust, automated assays with proven portability ‐ ideal for global multi-user, multi-instrument.

Precision analytics for oligonucleotide, cell and gene therapy

As the technologies for manufacturing and analysis of gene therapy products continue to evolve, it’s important to keep up to date on growing industry trends and analytical advancements. This compendium can help advance your gene therapies and oligonucleotide therapeutics to the next level with the latest tools and methods.

Multiple biotherapeutic characterizations on one platform

Biologics characterization results you can trust

Characterize molecules, large or small, with industry and regulatory-compliant capillary electrophoresis (CE) technology. It’s why every biopharmaceutical company with therapeutic IgGs currently on the market uses SCIEX CE systems in their bioanalytical processes.

Transferability

Develop and lock-down methods applicable to QC or regulated environments. Easily transfer methods using robust, automated assays with proven portability. Perfect for the global multi-user and multi-instrument organizations.

EZ-CE cartridge

The convenient EZ-CE cartridge is ready-to-use across multiple applications. Enhance data consistency and minimize staff training with the PA 800 Plus EZ-CE cartridge.

Trusted, fast and accurate biotherapeutic characterization

Industry and regulatory proven CE technology

The PA 800 Plus system is a robust analytical platform so you can have confidence in your data, without sacrificing speed.

Increase Efficiency

Running multiple assays on one platform simplifies staff training, maximizes ROI and reduces laboratory footprint.

Trust your assays

Extensive intercompany studies demonstrate the robustness of SCIEX capillary electrophoresis assays, encompassing CE-SDS, cIEF, CZE, and glycan analysis.

Simplify compliance

The USP has included validated procedures and system suitability criteria for IgG purity and heterogeneity analysis by capillary electrophoresis.

Maximize sensitivity

Achieve as low as 0.01% impurity detection, with at least three orders of magnitude of impurity, with modular detection (UV, PDA, LIF)

You can do all this on your PA 800 Plus system

Automated and quantitative analysis of biotherapeutics

Generate highly reproducible data with PA 800 Plus system quantitative and automated solutions. Our engineers have collaborated closely with biopharma development and QC groups experienced in biologics characterization to create assays that address your analytical challenges.

Protein purity analysis

Quantitative protein purity analysis with SDS-gel capillary electrophoresis

Glycan analysis

Run glycans faster than HILIC with simplified sample preparation, rapid, high-resolution separations, and automated glycan identification.

Charge heterogeneity

Conduct advanced capillary isoelectric focusing (cIEF) and hi-speed capillary zone electrophoresis (CZE) for charge heterogeneity analysis.

Peptide/Protein characterization

Analyze peptide and protein biotherapeutics beyond mAbs.

Gene therapy and nucleic acid analysis

Analyze a variety of single-stranded and double-stranded oligos and fragments with kit-based solutions.

Run glycans faster than HILIC

Empower your PA 800 Plus system with the SCIEX Empower Driver version 1.3

Take control of your PA 800 Plus system with Waters Empower 3 chromatography data software (CDS) for data integrity compliance. Reduce regulatory risk and maximize method transfer efficiency with the newly released SCIEX Empower Driver version 1.3.

Windows 10 upgrade

We are now Windows 10 compatible! Upgrade your SCIEX CE instruments today.

Related products

Kit-based assays

Get the reagents you need to run your experiments with one of the broadest selections of kit-based assays.

EZ-CE cartridge

The convenient EZ-CE cartridge is ready-to-use across multiple applications.

Connect to a mass spec

Integrate CE with electrospray ionization (ESI) and connect to a mass spec with the CESI 8000 Plus system.

Specifications  
PA 800 Plus system (P/N: A66528) Includes separation module with UV, photodiode array and LIF detection and system controller with PA 800 Plus software.
PA 800S Plus system (P/N: A66527) Includes separation module with photodiode array detection and system controller with PA 800 Plus software.
Dimensions Height: 29.2 in (74.2 cm)
Door open: 38.8 in (98.6 cm)
Width: 25 in (63.5 cm)
Depth: 28.4 in (72.1 cm)
Weight (uncrated) 188 lbs (85.3 kg) (includes photodiode array detection)
Electrical requirements Voltage: 100 - 240 V; 50/60 Hz
Voltage range 1 to 30 kV programmable at 0.1 kV increments
Current range 3 to 300 μA programmable at 0.1 μA increments
Pressure delivery range -5 to +100 PSI
Sample temperature control 4 - 60°C
Capillary temperature control 15 - 60°C
Sample trays: 2 x 96-well plates 2 x 48 universal vials 2 x 48 0.2 mL microvials
Buffer tray 2 x 36 universal vials
Detection capability UV/Vis
200, 214, 254, 280 nm standard filter
190 - 600 nm (custom filter option)
Diode array
190 - 600 nm (programmable)
0.5 - 32 Hz scan collection frequency (programmable)
Laser induced fluorescence (LIF)
300 - 700 nm excitation range
350 - 750 nm emission range
0 - 1000 RFU
Source lasers with 3 mW power output (P/N: A66528) 488 nm solid-state laser (included in P/N: A66528)

Resources

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Proven answers for protein, vaccine and gene therapy analysis webinar

Low-level quantitation of impurities in bispecific proteins using CE-SDS with LIF detection

High-precision lentivirus titer determination and protein profiling

Size distribution analysis of residual host cell DNA fragments in lentivirus by CGE-LIF

Rapid CZE analysis of erythropoietin variants

Sensitive impurity analysis of AAV capsid proteins by CE using Chromeo P503 fluorescent (ES)

Capillary electrophoresis in quality control: Part I: Application for therapeutic proteins

Achieving robust cIEF analysis of monoclonal antibodies while increasing capillary run-life and maintaining high resolution and reproducibility

Assay of IgG purity and heterogeneity using high-resolution sodium dodecyl sulfate capillary gel electrophoresis

Determination of full, partial and empty capsid ratios for adeno-associated virus (AAV) analysis

Sensitive AAV capsid protein impurity analysis by CE using P503 dye

Preparation scale-up of complex biological samples for deep N-glycomic analysis by CE-LIF and CESI-MS

IgG purity/heterogeneity and SDS-MW assays with 96-well plate adaption

Purity analysis of adeno-associated virus (AAV) capsid proteins using CE-SDS method

Native state charge variant analysis of commercialized monoclonal antibodies in minutes

Genome sizing methodology for genome integrity analysis of adeno-associated viruses (AAV)

Automation of CE-SDS for IgG purity/heterogeniety

Comparative characterization of the Fc domain N-glycosylation in monoclonal antibody and fusion protein therapeutics

Ultrafast Analysis of Human Milk Oligosaccharides (HMOs)

Capillary electrophoresis in quality control: Part II: CE-SDS: Method development and robustness

Separation of recombinant human erythropoietin (rhEPO) using the European Pharmacopoeia method on the PA 800 plus system

Velocity-corrected area calculation: SCEX PA 800 plus system Empower driver version 1.3 vs. 32 Karat software

Fast glycan sequencing using a fully automated carbohydrate sequencer

Purity analysis of adeno-associated virus (AAV) capsid proteins using CE-LIF technology

Evaluation of the glycosimilarity of biotherapeutics (ES)

Optimize non-reduced CE-SDS analysis with the SCIEX low pH SDS sample buffer

SDS-MW calibration and molecular weight estimation using the SCIEX PA 800 Plus Empower driver version 1.3

Optimization of a robust and sensitive capillary electrophoresis laser induced flourescence method for monitoring plasmid stability and purity

A single analytical platform for glycan analysis, charge heterogeneity, and purity determination of the NIST mAb

Rapid CZE analysis of erythropoietin variants (ZH)

Heterogeneity analysis of cysteine-linked antibody-drug conjugate by CE method (ZH)

Analyzing the average degree of substitution of betadex sulfobutyl ether sodium by capillary electrophoresis (ZH)

Analysis of purity and isoelectric points of HPV by capillary electrophoresis (ZH)

Analysis of Poly A tail distribution of mRNA by capillary gel electrophoresis (ZH)

Purity analysis of monoclonal antibodies (5-TAMRA labeling) by CE-SDS-LIF (ZH)

SCIEX CE solutions in China Pharmacopoeia (ZH)

Quantification of whey protein content in infant formula by CE-SDS (ZH)

Multi-laboratory validation for the purity analysis of protein products in CE-SDS method using CESI 8000 Plus (ZH)

The determination of α-lactalbumin and β-lactoglobulin in dairy by capillary zone electrophoresis (ZH)

Purity analysis of MRNA vaccine by using CGE-LIF (ZH)

The determination of lactoferrin in dairy by capillary zone electrophoresis (ZH)

CE Training Overview 2021

LC-MS/MS 技术平台

SCIEX solutions for gene therapy and oligonucleotide compendium

CE Training Overview DE 2021

Viral vector characterization: empty/full, transgene integrity and capsid protein analyses on a single platform webinar

Drug antibody ratio (ADC) calculations made easy and simple webinar

Genome integrity analysis of adeno-associated viruses (AAV) by genome sizing methodology webinar

How are CE methods being adapted to analyze new modalities webinar

Peptide therapeutics analysis by Capillary Electrophoresis

Low pH SDS sample buffer flyer (ZH)

CZE Rapid Charge Variant Analysis kit flyer (ZH)

PA 800 Plus system start-up consumable groups flyer

PA 800 Plus system Empower driver notes de version (FR)

PA 800 Plus system Empower driver notas de la versión (ES)

PA 800 Plus system Empower driver 版本发布说明 (ZH)

PA 800 Plus system Empower driver リリースノート (JA)

PA 800 Plus system Empower driver note di rilascio (IT)

PA 800 Plus system Empower driver release notes

PA 800 Plus system Empower driver user guide

PA 800 Plus system maintenance guide (FR)

PA 800 Plus system administration guide (ES)

PA 800 Plus system maintenance guide (ES)

PA 800 Plus system administration guide (FR)

PA 800 Plus system Empower driver user guide (ES)

PA 800 Plus system software validation guide

PA 800 Plus system Empower driver user guide (IT)

PA 800 Plus system Empower driver user guide (JA)

PA 800 Plus system Empower driver user guide (ZH)

PA 800 Plus system Empower driver user guide (FR)

PA 800 Plus system IgG Purity and Heterogeneity Assay kit application guide (JA)

PA 800 Plus system IgG Purity and Heterogeneity Assay kit application guide (ZH)

PA 800 Plus system SDS-MW Analysis kit application guide

PA 800 Plus system CZE Rapid Charge Variant Analysis kit application guide (ZH)

PA 800 Plus system ssDNA 100-R kit application guide

PA 800 Plus system CZE Rapid Charge Variant Analysis kit application guide

PA 800 Plus system SDS-MW Analysis kit application guide (ZH)

PA 800 Plus system LIFluor™ EnhanCE dsDNA 1000 Dye application guide

PA 800 Plus system CZE Rapid Charge Variant Analysis kit application guide (JA)

PA 800 Plus system brochure (ES)

Analysis of capping efficiency of MRNA by capillary gel electrophoresis (ZH)

Size analysis of residual host cell DNA in Lentivirus by CGE-LIF

Isoelectric points analysis of LNP encapsulated mRNA by cIEF (ZH)

Quality control of raw materials of diagnostic reagent in vitro by capillary electrophoresis (ZH)

Characterization of lentiviral vectors using capillary electrophoresis platform technology webinar

PA 800 Plus system Fast Glycan Labeling and Analysis kit application guide

Determination of P-carboxybenzaldehyde and P-methyl benzoic acid in P-terephthalic acid by capillary electrophoresis (ZH)

PA 800 Plus system IgG Purity and Heterogeneity Assay kit application guide

Capillary Electrophoresis kit brochure

RNA 9000 Purity and Integrity kit flyer

PA 800 Plus system SDS-MW Analysis kit application guide (JA)

PA 800 Plus system Empower driver versionshinweise (DE)

SCIEX Capillary Electrophoresis Consumables flyer

CE Grade Water flyer

PA 800 Plus system Empower driver flyer

CE instrument maintenance flyer

Fast Glycan labeling and analysis kit flyer

Capillary electrophoresis Windows 10 flyer

Analysis of Purity and Integrity of mRNA Vaccine by Capillary Gel Electrophoresis (ZH)

PA 800 Plus system RNA 9000 Purity and Integrity kit application guide

High-throughput LNP-mRNA Integrity Profiling webinar

Purity analysis of oligonucleotides by capillary gel electrophoresis (ZH)

Identification and quantitative analysis of impurity E in glutathione by capillary electrophoresis (ZH)

PA 800 Plus system overview guide (FR)

PA 800 Plus system overview guide

PA 800 Plus system overview guide (ZH)

PA 800 Plus system overview guide (ES)

PA 800 Plus system overview guide (DE)

Fast Glycan Labeling and Analysis kit flyer

PA 800 Plus system Empower driver flyer (ZH)

Low pH SDS sample buffer flyer

Capillary electrophoresis consulting flyer

Capillary electrophoresis solutions for DNA/mRNA vaccines flyer (ZH)

Low pH phosphate SDS sample buffer flyer (ZH)

Capillary electrophoresis training course flyer

CE-SDS training flyer

PA 800 Plus system brochure

CZE Rapid Charge Variant Analysis kit flyer

Fully automated sample preparation with ultrafast N-glycosylation analysis of therapeutic antibodies poster

A holistic approach to understanding cIEF poster

SCIEX solutions for LNP-based genetic medicines brochure

A sensitive and robust plasmid analysis method by capillary electrophoresis-laser induced fluorescence (Pfizer and SCIEX) poster

PA 800 Plus system LIFluor™ EnhanCE dsDNA 1000 Dye application guide (JA)

PA 800 Plus system LIFluor™ EnhanCE dsDNA 1000 Dye application guide (ZH)

PA 800 Plus system ssDNA 100-R kit application guide (JA)

PA 800 Plus system ssDNA 100-R kit application guide (ZH)

PA 800 Plus system Empower driver user guide (DE)

Comprehensive method development for a wide size range of RNA

Accurate analysis of double stranded DNA over an extended size range

Assessing glycosimilarity of biotherapeutics

High efficiency evaporative fluorophore labeling of glycans

Simple, fast and robust method for plasmid purity testing and degradation monitoring

Simplifying CE-SDS data processing with mineral oil

Comparation the N-Glycan of different sources recombinant human erythropoietin by using capillary electrophoresis with laser-induced fluorescence detector (ZH)

Determination of free carrier proteins in polysaccharideprotein conjugate vaccines by capillary micellar electrokinetic chromatography (ZH)

RNA 9000 purity and integrity kit methods

Best practices for using the PA 800 Plus with CE-SDS (JA) video

Tips and Tricks for using the PA 800 Plus (JA) video

Sample Preparation for the Fast Glycan Labeling and Analysis kit on the PA 800 Plus system video

PA 800 Plus system system maintenance guide

High Throughput Detection of IgG Purity by Using Quick Rinse CE-SDS Separation Method

dsDNA 1000 Kit Application Guide

CZE Rapid Charge Variant Analysis kit methods

A Series of Collaborations Between Various Pharmaceutical Companies and Regulatory Authorities Using CE

PA 800 Plus system Capillary Isoelectric Focusing (cIEF) Analysis kit application guide (JA)

PA 800 Plus system Capillary Isoelectric Focusing (cIEF) Analysis kit application guide

PA 800 Plus system Capillary Isoelectric Focusing (cIEF) Analysis kit application guide (ZH)

毛细管电泳法用于重组人血清白蛋白分子大小变异体和等电点分析

Method_Optimization.pdf

CZE Method

CZE Capillary Conditioning

CZE Shutdown

CZE Analysis

High-Resolution cIEF of Therapeutic Monoclonal Antibodies

High-throughput LNP-mRNA integrity profiling webinar

Learning Center  
Installing the LIF Aperture and Probe Guide/Stabilizer Step-by-Step Video Guide Watch
Cartridge Removal, Rebuild and Reinstallation Step-by-Step Video Guide Watch
Change the Coolant Tubing Step-by-Step Video Guide Watch
Change a Detector Step-by-Step Video Guide Watch
Capillary Removal Step-by-Step Video Guide Watch
Capillary Storage Step-by-Step Video Guide Watch
Fast Glycan Software Download